公开(公告)号：DE4408891A1

公开(公告)日：1995-09-21

申请号：DE4408891

申请日：1994-03-16

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  BIALOJAN SIEGFRIED DR ,  ENDLER-JOBST ANNI BARBARA DR

IPC: C12N15/09 ,  C07K14/705 ,  C12N5/10 ,  C12Q1/02 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/6897 ,  C12Q1/56

公开(公告)号：DE4209110A1

公开(公告)日：1993-05-27

申请号：DE4209110

申请日：1992-03-20

Applicant: BASF AG

Inventor： STRUBE KARL-HERMANN DR ,  BIALOJAN SIEGFRIED DR ,  KROEGER BURKHARD DR ,  FRIEDRICH THOMAS DR

IPC: A61K38/55 ,  A61K38/00 ,  A61P7/02 ,  C07K14/00 ,  C07K14/435 ,  C07K14/81 ,  C07K14/815 ,  C12N15/09 ,  C12N15/15 ,  C12P21/02 ,  C12R1/19

Abstract: Described are new thrombin-inhibiting proteins obtained from terrestrial leeches of the species Haemadipsa and with the amino-acid sequence Ile-Arg-Phe-Gly-Met-Gly-Lys-Val-Pro-Cys-Pro-Asp-Gly-Glu-Val-Gly-Tyr-Th r-Cys-Asp-Cys-Gly-Glu-Lys-Ile-Cys-Leu-Tyr-Gly-Gln-Ser-Cys-Asn-Asp-Gly- Gln-Cys-Ser-Gly-Asp-Pro-Lys-Pro-Ser-Ser-Glu-Phe-Glu-Glu-Phe-Glu-Ile-As p-Glu-Glu-Glu-Lys or an amino-acid sequence obtained by C-terminal shortening the above sequence by one to twelve amino acids. The proteins are suitable for use against illnesses. Also described are the nucleic acids coding for these proteins.

公开(公告)号：DE4408690A1

公开(公告)日：1995-09-21

申请号：DE4408690

申请日：1994-03-15

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  BIALOJAN SIEGFRIED DR ,  BOLLSCHWEILER CLAUS DR

IPC: C12N15/09 ,  C12N15/65 ,  C12N15/79 ,  C12Q1/02 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/6897

公开(公告)号：DE4400745A1

公开(公告)日：1995-07-20

申请号：DE4400745

申请日：1994-01-13

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  BIALOJAN SIEGFRIED DR

IPC: C07K14/75 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/6897

Abstract: Substances (I) that inhibit expression of beta-fibrinogen (II) are identified by (1) transfecting a host cell with a recombinant vector that contains the regulatory region of the (II) gene functionally coupled to a reporter gene (RG); (2) either (a) isolating cells that carry the vector transiently or (b) generating cell lines that have stably integrated the vector; (3) exposing these cells to test cpds. and (4) measuring expression of RG.

公开(公告)号：DE4234921A1

公开(公告)日：1994-04-21

申请号：DE4234921

申请日：1992-10-16

Applicant: BASF AG

Inventor： STRUBE KARL-HERMANN DR ,  MEYER THOMAS DR ,  BIALOJAN SIEGFRIED DR ,  KROEGER BURKHARD DR

IPC: A61K38/00 ,  C07K14/815 ,  C07K15/08 ,  A61K37/02

Abstract: Described is a new anticoagulant protein from land leeches of the genus Haemadipsa, having the N-terminal amino acid sequence: A-Val-Lys-Phe-Cys-Gly-His-Pro-Leu-Ser-Leu-Pro-Thr-Ala-Leu-Cys-Ala, where A represents an indefinite amino acid. This protein can be used in combatting diseases.

公开(公告)号：DE4412372A1

公开(公告)日：1995-10-19

申请号：DE4412372

申请日：1994-04-12

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  SEULBERGER HARALD DR ,  SUBKOWSKI THOMAS DR ,  HILLEN HEINZ DR ,  JACOB ELARD DR ,  MEYER THOMAS DR ,  OTTER RAINER DR ,  SCHMIDT MARTIN DR

IPC: A61K38/00 ,  C12N9/64 ,  C12N15/63 ,  C12N9/48 ,  C12N9/99 ,  C12N15/57 ,  C12N15/79 ,  C12N15/85

Abstract: An endothelin converting enzyme (ECE) having the 754 (bovine) or 753 (human) amino acid sequence shown in the specification, or functional fragments, is new. Also claimed is DNA defined as (a) coding for the ECE as above, (b) having the 2314 (bovine) or 2720 (human) nucleotide sequence given in the specification or complementary sequences, or (c) encoding proteins which are recognised by antibodies generated against ECE as above.

公开(公告)号：DE4339100A1

公开(公告)日：1995-05-18

申请号：DE4339100

申请日：1993-11-16

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  SEULBERGER HARALD DR ,  MEYER THOMAS DR ,  SCHMIDT MARTIN DR ,  JACOB ELARD DR ,  OTTER RAINER DR ,  SUBKOWSKI THOMAS DR ,  HILLEN HEINZ DR

IPC: A61K38/00 ,  C12N9/64 ,  C12N9/48 ,  C12N5/06 ,  C12N9/99 ,  C12N15/63 ,  C12Q1/37

Abstract: Endothelin converting enzyme (ECE) having mol.wt. 250000 Da and a partial amino acid sequence with at least 80% homology to sequence (I) is new: Xaa-Xaa-Pro-Asn-Ala-Leu-Asn-Phe-Gly-Gly-Ile-Gly-Val-Val-Val-Gly-His-Gl u-Leu-Thr-His-Ala-Phe- (I) Also claimed is a method for the isolation of ECE by (i) stimulation of mammalian cells for overproduction of ECE with an inhibitor of ECE, and (ii) isolation of the ECE. The pref. cell line is a bovine cell line FBHE and the pref. inhibitor is phosphoramidon (PHAM).

公开(公告)号：DE4136513A1

公开(公告)日：1993-05-13

申请号：DE4136513

申请日：1991-11-06

Applicant: BASF AG

Inventor： FRIEDRICH THOMAS DR ,  BIALOJAN SIEGFRIED DR ,  KROEGER BURKHARD DR ,  KUENAST CHRISTOPH DR

IPC: A61K35/64 ,  A61K38/00 ,  A61K38/55 ,  A61P7/02 ,  C07K14/00 ,  C07K14/245 ,  C07K14/435 ,  C07K14/81 ,  C12N15/15 ,  C12P21/02 ,  C12R1/19

Abstract: The description relates to a novel thrombin inhibiting protein from reduvii with a molecular weight of some 12000 Dalton and the N-terminus Glu-Gly-Gly-Glu-Pro-Cys-Ala-Cys-Pro-His-Ala-Leu-His-Arg-Val-Cys-Gly-Se r-Asp. The protein is useful in combatting diseases.