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    New nucleic acid encoding L-pantolactone hydrolase, useful for producing pure D-pantolactone, an intermediate for vitamins, from its racemate
    15.
    发明专利
    New nucleic acid encoding L-pantolactone hydrolase, useful for producing pure D-pantolactone, an intermediate for vitamins, from its racemate 未知

    公开(公告)号：DE19952501A1

    公开(公告)日：2001-05-03

    申请号：DE19952501

    申请日：1999-10-29

    Applicant: BASF AG

    Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

    IPC: C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00

    Abstract: Isolated nucleic acid (I), encoding a polypeptide with L-pantolactone hydrolase (LPH) activity comprising a 810 base pair sequence (1), equivalents of (1) within the degeneracy of the genetic code, is new. All sequences are fully defined in the specification. Independent claims are also included for the following: (a) derivatives of (1) that encode a polypeptide with at least 50% homology with a 269 amino acid sequence (2), without significant loss of enzymatic activity; (b) polypeptides (II) encoded by (I); (c) nucleic acid constructs containing (I) linked to one or more regulatory elements; (d) vector containing (I) or the construct of (b); (e) microorganism containing (I), the construct of (b) or the vector of (c); (f) LPH (IIa) with specified properties; and (g) production of D-pantolactone (III) by treating racemic pantolactone with (IIa) or the microorganisms of (d). All sequences are fully defined in the specification.

    16.
    发明专利
    未知

    公开(公告)号：NO20011912D0

    公开(公告)日：2001-04-18

    申请号：NO20011912

    申请日：2001-04-18

    Applicant: BASF AG

    Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

    IPC: C07H21/00 ,  C12N1/21 ,  C12N15/09 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12N

    Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

    New nucleic acid sequence encoding Alcaligenes faecalis nitrilase polypeptide useful for converting racemic nitriles to chiral carboxylic acids
    19.
    发明专利
    New nucleic acid sequence encoding Alcaligenes faecalis nitrilase polypeptide useful for converting racemic nitriles to chiral carboxylic acids 未知

    公开(公告)号：DE19848129A1

    公开(公告)日：2000-04-20

    申请号：DE19848129

    申请日：1998-10-19

    Applicant: BASF AG

    Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

    IPC: C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12N15/63 ,  C07H21/04

    Abstract: An isolated nucleic acid sequence (I) encoding an Alcaligenes faecalis nitrilase polypeptide is new. An isolated nucleic acid sequence (I) encoding a polypeptide with nitrilase activity is selected from: (1) a defined genomic DNA sequence (seq1) of 1071 base pairs (bp) given in the specification; (2) nucleic acid sequences derived from seq1 as a result of the degeneracy of the genetic code; and (3) derivatives of seq1 that code for a polypeptide with a defined sequence of 356 amino acids and have at least 95% homology at the amino acid level without essentially reducing the enzymatic activity of the polypeptide (sic). Independent claims are also included for: (1) an amino acid sequence encoded by (I); (2) a nucleic acid construct (II) comprising (I) linked to one or more regulatory sequences; (3) a vector containing (I) or (II); (4) a microorganism containing (I) or (II); (5) a process for preparing chiral carboxylic acids of formula (III), comprising converting a racemic nitrile of formula (IV) in the presence of an amino acid sequence as in (1) or a growing, resting or digested microorganism as in (4), where the nitrile conversion is at least 25 mmoles/hour per mg protein or 25 mmoles/hour per g dry weight. R -R = H, optionally substituted 1-10C alkyl, 2-10C alkenyl, optionally substituted aryl, heteroaryl, OR or NR R , provided that R -R are all different; R = H, optionally substituted 1-10C alkyl, 2-10C alkenyl, 2-11C alkanoyl, 3-11C alkenoyl, aryl, aroyl, heteroaryl or heteroaroyl; and R = H, optionally substituted 1-10C alkyl, 2-10C alkenyl, aryl or heteroaryl.

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