公开(公告)号：CZ139496A3

公开(公告)日：1997-10-15

申请号：CZ139496

申请日：1994-11-10

Applicant: BASF AG

Inventor： KROGER BURKHARD DR ,  SEULBERGER HARALD DR ,  MEYER THOMAS DR ,  SCHMIDT MARTIN DR ,  JACOB ELARD DR ,  OTTER RAINER DR ,  SUBKOWSKI THOMAS DR ,  HILLEN HEINZ DR

IPC: G01N33/573 ,  A01K67/027 ,  A61K31/70 ,  A61K35/12 ,  A61K38/00 ,  A61K38/43 ,  A61K38/48 ,  A61K39/395 ,  A61K48/00 ,  A61P9/00 ,  A61P9/08 ,  A61P9/10 ,  A61P9/12 ,  A61P11/00 ,  A61P13/02 ,  A61P15/00 ,  A61P25/04 ,  A61P25/28 ,  A61P29/00 ,  A61P31/04 ,  C07H21/04 ,  C07K14/47 ,  C07K16/40 ,  C12N5/00 ,  C12N5/10 ,  C12N9/64 ,  C12N15/09 ,  C12N15/113 ,  C12N15/57 ,  C12R1/91 ,  C12N15/11

Abstract: PCT No. PCT/EP94/03706 Sec. 371 Date May 16, 1996 Sec. 102(e) Date May 16, 1996 PCT Filed Nov. 10, 1994 PCT Pub. No. WO95/14095 PCT Pub. Date May 26, 1995The invention relates to endothelin-converting enzymes containing the polypeptide sequence described in SEQ. ID. NO. 30 or SEQ. ID. NO. 36 or functional fragments thereof, genes which code for such enzymes, and processes for producing the said enzymes and the use thereof.

公开(公告)号：DE59207401D1

公开(公告)日：1996-11-28

申请号：DE59207401

申请日：1992-04-24

Applicant: BASF AG

Inventor： GRAMMENOS WASSILIOS DR ,  KIRSTGEN REINHARD DR ,  OBERDORF KLAUS DR ,  SAUTER HUBERT DR ,  ROEHL FRANZ DR ,  OTTER RAINER DR ,  AMMERMANN EBERHARD DR ,  LORENZ GISELA DR ,  KARDORFF UWE DR ,  KUENAST CHRISTOPH DR

IPC: A01N35/10 ,  A01N43/54 ,  A01N43/56 ,  A01N43/78 ,  C07C59/64 ,  C07C69/145 ,  C07C69/618 ,  C07C69/65 ,  C07C69/73 ,  C07C69/732 ,  C07C69/734 ,  C07C69/738 ,  C07C69/76 ,  C07C69/767 ,  C07C69/92 ,  C07C69/94 ,  C07C251/48 ,  C07C251/52 ,  C07C251/54 ,  C07C323/62 ,  C07D207/337 ,  C07D209/18 ,  C07D209/30 ,  C07D213/64 ,  C07D231/12 ,  C07D231/18 ,  C07D235/26 ,  C07D235/28 ,  C07D239/26 ,  C07D239/34 ,  C07D239/38 ,  C07D239/46 ,  C07D239/52 ,  C07D239/56 ,  C07D239/60 ,  C07D239/88 ,  C07D239/90 ,  C07D261/08 ,  C07D261/10 ,  C07D261/12 ,  C07D261/20 ,  C07D263/16 ,  C07D263/38 ,  C07D263/40 ,  C07D263/46 ,  C07D263/56 ,  C07D263/58 ,  C07D271/10 ,  C07D271/113 ,  C07D275/02 ,  C07D277/20 ,  C07D277/30 ,  C07D277/32 ,  C07D277/34 ,  C07D277/36 ,  C07D277/64 ,  C07D277/74 ,  C07D285/08 ,  C07D285/12 ,  C07D285/125 ,  C07D307/54 ,  C07D317/30 ,  C07D333/24 ,  C07F9/40

Abstract: alpha -Phenylacrylic acid derivatives I in which n = 0 to 4; y = 0 or 1; R = H; alkyl; alkenyl; alkynyl; optionally substituted cycloalkyl; vinyl or ethynyl if W represents a direct bond; R = CN; alkenyl; alkynyl; optionally substituted cycloalkyl; optionally substituted alkyl; R = H; NO2; CN; halogen; alkyl; alkoxy; haloalkyl; haloalkoxy; alkylthio; or, in the event that n represents 2, 3 or 4, two adjacent substituents R together are optionally substituted 1,3-butadiene-1,4-diyl; R = H; CHO; optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; an optionally substituted saturated or unsaturated cyclic radical; (phenyl)3P-; (phenyl)2PO-; (alkoxy)2PO-; an optionally substituted (hetero)aromatic system; W = a direct bond; oxygen; sulphur; optionally substituted nitrogen; A = -O-; -C(=O)-; -O-C(=O)-; -C(=O)-O-; -S-; -S(=O)-; -O-S(=0)--S(=O)-O-; -S(=O)2-; -O-S(=O)2-; -S(=O)2-O-; -NR -; -O-NR -; -NR -C(=O)-; -C(=O)-NR -; -NR -C(=O)-NR -; -NR -S(=O)-; -S(=O)-NR -; -NR -S-(=O)-NR -; -NR -S(=O)2-; -S(=O)2-NR -; -NR -S(=O)2-NR -; -N=N-; -NR -NR -; -NR -NR -C(=O)-; -C(=O)-NR -NR -; -NR -NR -S(=O)-; -S(=O)-NR -NR -; -NR -NR -S(=O)2-; -S(=O)2-NR -NR -; -O-(alkylene)-O-; -C-(=O)-(alkylene)-O-; -O-C(=O)-(alkylene)-O-; -C(=O)-O-(alkylene)-O-; -S-(alkylene)-O-; -S(=0)2-(alkylene)-O-; -O-S-(=O)2-(alkylene)-O-; -S(=O)2-O-(alkylene)-O-; -NR -(alkylene)-O-; -O-NR -(alkylene)-O-; -NR -C(=O)-(alkylene)-O-; -C(=O)-NR -(alkylene)-O-; -NR -C(=O)-NR -(alkylene)-O-; -N=N-(alkylene)-O-; -NR -NR -(alkylene)-O-; -NR -NR -C(=O)-(alkylene)-O-; -C(=O)-NR -NR -(alkylene)-O-; optionally substituted alkylene, alkenylene or alkynylene, it being possible for these carbon chains to be interrupted by one of the following groups or to be bonded to R or to the phenyl ring by one of the following groups: O, S, NR , CO, CO-O, O-CO, SO, SO2, SO2-O, O-SO2, NR -CO, CO-NR , NR -CO-NR , N=N, NR -NR , NR -NR -CO, CO-NR -NR ; R , R = H, alkyl or alkoxy, their preparation, fungicides and pesticides containing them, and their use.

公开(公告)号：DE4412372A1

公开(公告)日：1995-10-19

申请号：DE4412372

申请日：1994-04-12

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  SEULBERGER HARALD DR ,  SUBKOWSKI THOMAS DR ,  HILLEN HEINZ DR ,  JACOB ELARD DR ,  MEYER THOMAS DR ,  OTTER RAINER DR ,  SCHMIDT MARTIN DR

IPC: A61K38/00 ,  C12N9/64 ,  C12N15/63 ,  C12N9/48 ,  C12N9/99 ,  C12N15/57 ,  C12N15/79 ,  C12N15/85

Abstract: An endothelin converting enzyme (ECE) having the 754 (bovine) or 753 (human) amino acid sequence shown in the specification, or functional fragments, is new. Also claimed is DNA defined as (a) coding for the ECE as above, (b) having the 2314 (bovine) or 2720 (human) nucleotide sequence given in the specification or complementary sequences, or (c) encoding proteins which are recognised by antibodies generated against ECE as above.

公开(公告)号：DE4339100A1

公开(公告)日：1995-05-18

申请号：DE4339100

申请日：1993-11-16

Applicant: BASF AG

Inventor： KROEGER BURKHARD DR ,  SEULBERGER HARALD DR ,  MEYER THOMAS DR ,  SCHMIDT MARTIN DR ,  JACOB ELARD DR ,  OTTER RAINER DR ,  SUBKOWSKI THOMAS DR ,  HILLEN HEINZ DR

IPC: A61K38/00 ,  C12N9/64 ,  C12N9/48 ,  C12N5/06 ,  C12N9/99 ,  C12N15/63 ,  C12Q1/37

Abstract: Endothelin converting enzyme (ECE) having mol.wt. 250000 Da and a partial amino acid sequence with at least 80% homology to sequence (I) is new: Xaa-Xaa-Pro-Asn-Ala-Leu-Asn-Phe-Gly-Gly-Ile-Gly-Val-Val-Val-Gly-His-Gl u-Leu-Thr-His-Ala-Phe- (I) Also claimed is a method for the isolation of ECE by (i) stimulation of mammalian cells for overproduction of ECE with an inhibitor of ECE, and (ii) isolation of the ECE. The pref. cell line is a bovine cell line FBHE and the pref. inhibitor is phosphoramidon (PHAM).

公开(公告)号：DE4313232A1

公开(公告)日：1994-10-27

申请号：DE4313232

申请日：1993-04-22

Applicant: BASF AG

Inventor： ECKARD PETER ,  HILLEN HEINZ DR ,  OTTER RAINER DR ,  SCHMIDT MARTIN DR ,  SUBKOWSKI THOMAS DR ,  ZECHEL JOHANN-CHRISTIAN DR

IPC: C07K14/575 ,  C12Q1/37 ,  C07K7/08 ,  G01N33/68

Abstract: The present invention relates to an assay method for identifying inhibitors of endothelin converting enzyme (ECE) and to substrates for use in this assay method.