公开(公告)号：DE602004021778D1

公开(公告)日：2009-08-13

申请号：DE602004021778

申请日：2004-02-24

Applicant: BASF SE

Inventor： ZELINSKI THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS

IPC: C12N9/78 ,  C12N15/55 ,  C12N15/82 ,  C12P7/40

Abstract: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

公开(公告)号：ES2367847T3

公开(公告)日：2011-11-10

申请号：ES06841443

申请日：2006-12-19

Applicant: BASF SE

Inventor： SCHAEDLER ANDREAS ,  FRIEDRICH THOMAS ,  STUERMER RAINER ,  RINCK-JAHNKE SABINE

IPC: C12P7/24 ,  C12N9/02

Abstract: Método para la producción de aldehídos o alcoholes saturados ópticamente activos de la fórmula (2) a partir de aldehídos αβ- insaturados de la fórmula (1) mediante reducción en presencia de una enoato-reductasa (i) con la secuencia de polipéptidos SEQ ID NO:1 o 2, o (ii) con una secuencia de polipéptidos la cual exhibe por lo menos 80% de identidad de secuencia con SEQ ID NO:1 o 2. donde R 1 y R 2 significan independientemente uno de otro H, alquilo C1-C4, R 3 significa H, alquilo o alquenilo C1-C6 en forma ramificada y no ramificada.

公开(公告)号：ES2357115T3

公开(公告)日：2011-04-18

申请号：ES04765718

申请日：2004-09-30

Applicant: BASF SE

Inventor： STUERMER RAINER ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  BREUER MICHAEL

IPC: C07D333/16 ,  C07D333/20 ,  C12N9/04 ,  C12N15/53 ,  C12N15/63 ,  C12P7/04

Abstract: Método para la producción de (S)-3-metilamino-1-(tien-2-il)-propan-1-ol de la fórmula I-S en el cual a) reacciona tiofeno con un halogenuro de ß-halopropionilo o un halogenuro de acriloilo en presencia de un ácido Lewis para dar una 3-halo-1-(tien-2-il)-propan-1-ona, donde se introduce un haluro de hidrógeno simultáneamente o después de terminada la reacción, aunque antes del aislamiento del producto de reacción, y b) se reduce la propanona obtenida en la etapa a) en presencia de una deshidrogenasa (E.C. 1.1.x.x.), la cual exhibe una selectividad respecto a la formación de (S)-3-metilamino-1-(tien-2-il)propan-1-ol, y a continuación, dado el caso, se hace reaccionar con metilamina sin aislamiento del producto de reacción.

公开(公告)号：DK1929001T3

公开(公告)日：2010-08-09

申请号：DK06806768

申请日：2006-09-14

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  FRIEDRICH THOMAS ,  KESSELER MARIA

IPC: C12N9/04

公开(公告)号：AT466075T

公开(公告)日：2010-05-15

申请号：AT06806768

申请日：2006-09-14

Applicant: BASF SE

Inventor： BREUER MICHAEL ,  FRIEDRICH THOMAS ,  KESSELER MARIA

IPC: C12N9/04

Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.

公开(公告)号：DE50115182D1

公开(公告)日：2009-11-26

申请号：DE50115182

申请日：2001-08-30

Applicant: BASF SE

Inventor： HAUER BERNHARD ,  FRIEDRICH THOMAS ,  NUEBLING CHRISTOPH ,  STUERMER RAINER ,  LADNER WOLFGANG

IPC: C12N15/09 ,  C12N15/55 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/16 ,  C12N9/18 ,  C12N15/63 ,  C12P7/00 ,  C12P7/62 ,  C12P41/00 ,  C12R1/38

Abstract: The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

公开(公告)号：AT445702T

公开(公告)日：2009-10-15

申请号：AT01962989

申请日：2001-08-30

Applicant: BASF SE

Inventor： HAUER BERNHARD ,  FRIEDRICH THOMAS ,  NUEBLING CHRISTOPH ,  STUERMER RAINER ,  LADNER WOLFGANG

IPC: C12N15/09 ,  C12N15/55 ,  C12N1/15 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21 ,  C12N5/10 ,  C12N9/16 ,  C12N9/18 ,  C12N15/63 ,  C12P7/00 ,  C12P7/62 ,  C12P41/00 ,  C12R1/38

Abstract: The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

公开(公告)号：AT435278T

公开(公告)日：2009-07-15

申请号：AT04713882

申请日：2004-02-24

Applicant: BASF SE

Inventor： ZELINSKI THOMAS ,  KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS

IPC: C12N9/78 ,  C12N15/55 ,  C12N15/82 ,  C12P7/40

Abstract: The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-type Alcaligenes faecalis comprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

公开(公告)号：ES2376250T3

公开(公告)日：2012-03-12

申请号：ES06763798

申请日：2006-06-20

Applicant: BASF SE

Inventor： HAERING DIETMAR ,  ANGEL MAXIMILIAN ,  HAEHNLE HANS-JOACHIM ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  BRAIG VOLKER ,  CHRISSTOFFELS LYSANDER ,  RUEBENACKER MARTIN

IPC: C08F6/10 ,  C08F6/00 ,  C08F20/54 ,  C08F26/02 ,  C12N9/80 ,  C12S99/00

Abstract: Procedimiento para la reducción del contenido en formamida de preparados (Z), que se obtienen a partir de monómeros de N-vinilformamida mediante polimerización, poniéndose en contacto una hidrolasa de clasificación de enzima E. C. 3.5.1.5. con (Z), reduciéndose el contenido en formamida a menos de 50 ppm.

公开(公告)号：AT513054T

公开(公告)日：2011-07-15

申请号：AT06841443

申请日：2006-12-19

Applicant: BASF SE

Inventor： SCHAEDLER ANDREAS ,  FRIEDRICH THOMAS ,  STUERMER RAINER ,  RINCK-JAHNKE SABINE

IPC: C12P7/24 ,  C12N9/02

Abstract: Preparation of an optically active saturated aldehyde or alcohol (II) comprises reduction of alpha , beta unsaturated aldehyde (I) with a polypeptide sequence (SEQ ID NO: 1 or 2), or a polypeptide sequence exhibiting at least 80% sequence identity of (SEQ ID NO: 1 or 2), in the presence of an enoate reductase. Preparation of an optically active saturated aldehyde or alcohol of formula (II) comprises reduction of alpha , beta unsaturated aldehyde (I) of formula (R 3>-C(R 2>)=C(R 1>)-CH(=O)) with a polypeptide sequence (SEQ ID NO: 1 or 2) (not defined), or a polypeptide sequence exhibiting at least 80% sequence identity of (SEQ ID NO: 1 or 2), in the presence of a enoate reductase. R 1>, R 2>H or 1-4C-alkyl; and R 3>H or 1-6C alkyl or alkenyl. [Image].