公开(公告)号：AU2012348122B2

公开(公告)日：2017-09-14

申请号：AU2012348122

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：AU2012348122A1

公开(公告)日：2014-06-19

申请号：AU2012348122

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：CA2857177A1

公开(公告)日：2013-06-13

申请号：CA2857177

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/564

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：AU2011308657A1

公开(公告)日：2013-04-18

申请号：AU2011308657

申请日：2011-09-30

Applicant: BIO RAD LABORATORIES

Inventor： LU YABIN ,  WALKER ROGER

IPC: G01N33/48

Abstract: Methods, systems, and apparatus for accurately determining a proportion (ratio) of two analytes is provided, as well as provide a concentration of a first analyte from a determined concentration of a second analyte and from a proportion of the analytes to each other. In one aspect, a surface model (called a dose surface herein) relating the concentrations of the two analytes to the proportion can be used to obtain accurate values for one of the variables (e.g. a concentration or the proportion) when the other two variables have previously been obtained. The dose surface can be a three-dimensional surface and be non-linear. The dose surface model can include multiple regression functions. For example, measured responses can be individually converted to concentrations using two dose-response curves, and the concentrations can be input to a dose surface function to obtain the proportion.

公开(公告)号：EP2316027A4

公开(公告)日：2011-11-09

申请号：EP09808625

申请日：2009-08-13

Applicant: BIO RAD LABORATORIES

Inventor： LIKUSKI ROBERT ,  WALKER ROGER ,  LU YABIN

IPC: G01N33/53 ,  G01N33/533

CPC classification number: G01N33/723 ,  G01N33/721

公开(公告)号：EP2625519A4

公开(公告)日：2014-03-05

申请号：EP11829945

申请日：2011-09-30

Applicant: BIO RAD LABORATORIES

Inventor： LU YABIN ,  WALKER ROGER

IPC: G01N33/72 ,  G01N33/48

CPC classification number: G01N33/723

公开(公告)号：EP2788760A4

公开(公告)日：2015-07-15

申请号：EP12855209

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  C07K14/415 ,  C12N9/10 ,  C12N15/54 ,  C12N15/62 ,  G01N33/564 ,  G01N33/68

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06