公开(公告)号：AU2009282958A1

公开(公告)日：2010-02-25

申请号：AU2009282958

申请日：2009-08-13

Applicant: BIO RAD LABORATORIES

Inventor： LIKUSKI ROBERT ,  WALKER ROGER ,  LU YABIN

IPC: G01N33/53 ,  G01N33/533

Abstract: The ratio of analytes is determined directly from the responses of the analytes using a conversion method. Individual analyte responses are obtained by using a selected measuring technique, and these individual responses are used as the independent variables in a conversion method. The dependent variable of conversion method is the desired analyte ratio. The resulting conversion method is then used to directly calculate the desired ratio of analytes as a function of the measured responses. No intermediate conversions, such as using a calibration curve to convert individual measured analyte responses to concentration values, are needed to obtain the desired ratio.

公开(公告)号：AU2011308657B2

公开(公告)日：2014-12-18

申请号：AU2011308657

申请日：2011-09-30

Applicant: BIO RAD LABORATORIES

Inventor： LU YABIN ,  WALKER ROGER

IPC: G01N33/48

Abstract: Methods, systems, and apparatus for accurately determining a proportion (ratio) of two analytes is provided, as well as provide a concentration of a first analyte from a determined concentration of a second analyte and from a proportion of the analytes to each other. In one aspect, a surface model (called a dose surface herein) relating the concentrations of the two analytes to the proportion can be used to obtain accurate values for one of the variables (e.g. a concentration or the proportion) when the other two variables have previously been obtained. The dose surface can be a three-dimensional surface and be non-linear. The dose surface model can include multiple regression functions. For example, measured responses can be individually converted to concentrations using two dose-response curves, and the concentrations can be input to a dose surface function to obtain the proportion.

公开(公告)号：WO2013085851A3

公开(公告)日：2014-08-14

申请号：PCT/US2012067639

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Abstract translation: 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白的六聚体形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过将麦醇溶蛋白融合蛋白固定在固体支持物上来制备抗原。 抗原可以进一步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。

公开(公告)号：WO2010021914A2

公开(公告)日：2010-02-25

申请号：PCT/US2009053746

申请日：2009-08-13

Applicant: BIO RAD LABORATORIES ,  LIKUSKI ROBERT ,  WALKER ROGER ,  LU YABIN

Inventor： LIKUSKI ROBERT ,  WALKER ROGER ,  LU YABIN

IPC: G01N33/53 ,  G01N33/533

CPC classification number: G01N33/723 ,  G01N33/721

Abstract: The ratio of analytes is determined directly from the responses of the analytes using a conversion method. Individual analyte responses are obtained by using a selected measuring technique, and these individual responses are used as the independent variables in a conversion method. The dependent variable of conversion method is the desired analyte ratio. The resulting conversion method is then used to directly calculate the desired ratio of analytes as a function of the measured responses. No intermediate conversions, such as using a calibration curve to convert individual measured analyte responses to concentration values, are needed to obtain the desired ratio.

Abstract translation: 分析物的比例直接由使用转化方法的分析物的反应确定。 通过使用选定的测量技术获得单个分析物响应，并且将这些单独的反应用作转化方法中的独立变量。 转化方法的因变量是所需的分析物比例。 然后使用所得的转化方法直接计算作为测量响应的函数的分析物的期望比例。 不需要中间转换，例如使用校正曲线将单个测量的分析物反应转化为浓度值，以获得所需的比例。

公开(公告)号：CA2857177C

公开(公告)日：2020-06-23

申请号：CA2857177

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/564

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：ES2688268T3

公开(公告)日：2018-10-31

申请号：ES16205467

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  C07K14/415 ,  C12N9/10 ,  C12N15/54 ,  C12N15/62 ,  G01N33/564 ,  G01N33/68

Abstract: Un antígeno para detectar la enfermedad celíaca que comprende una proteína gliadina desamidada recombinante o sintética que comprende un hexámero de péptidos, en el que cada péptido tiene la secuencia de SEQ ID NO: 1.

公开(公告)号：CA2812956A1

公开(公告)日：2012-04-05

申请号：CA2812956

申请日：2011-09-30

Applicant: BIO RAD LABORATORIES

Inventor： LU YABIN ,  WALKER ROGER

IPC: G01N33/48

Abstract: Methods, systems, and apparatus for accurately determining a proportion (ratio) of two analytes is provided, as well as provide a concentration of a first analyte from a determined concentration of a second analyte and from a proportion of the analytes to each other. In one aspect, a surface model (called a dose surface herein) relating the concentrations of the two analytes to the proportion can be used to obtain accurate values for one of the variables (e.g. a concentration or the proportion) when the other two variables have previously been obtained. The dose surface can be a three-dimensional surface and be non-linear. The dose surface model can include multiple regression functions. For example, measured responses can be individually converted to concentrations using two dose-response curves, and the concentrations can be input to a dose surface function to obtain the proportion.

公开(公告)号：CA2734741A1

公开(公告)日：2010-02-25

申请号：CA2734741

申请日：2009-08-13

Applicant: BIO RAD LABORATORIES

Inventor： LIKUSKI ROBERT ,  WALKER ROGER ,  LU YABIN

IPC: G01N33/53 ,  G01N33/533

Abstract: The ratio of analytes is determined directly from the responses of the analytes using a conversion method. Individual analyte responses are obtained by using a selected measuring technique, and these individual responses are used as the independent variables in a conversion method. The dependent variable of conversion method is the desired analyte ratio. The resulting conversion method is then used to directly calculate the desired ratio of analytes as a function of the measured responses. No intermediate conversions, such as using a calibration curve to convert individual measured analyte responses to concentration values, are needed to obtain the desired ratio.

公开(公告)号：AU2017272223B2

公开(公告)日：2019-05-09

申请号：AU2017272223

申请日：2017-12-06

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. 9765390_1 (GHMatters) P97180.AU.1

公开(公告)号：AU2017272223A1

公开(公告)日：2018-01-04

申请号：AU2017272223

申请日：2017-12-06

Applicant: BIO-RAD LABORATORIES INC

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. 9765390_1 (GHMatters) P97180.AU.1