公开(公告)号：WO2013085851A3

公开(公告)日：2014-08-14

申请号：PCT/US2012067639

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Abstract translation: 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白的六聚体形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过将麦醇溶蛋白融合蛋白固定在固体支持物上来制备抗原。 抗原可以进一步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。

公开(公告)号：CA2857177C

公开(公告)日：2020-06-23

申请号：CA2857177

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/564

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：ES2688268T3

公开(公告)日：2018-10-31

申请号：ES16205467

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  C07K14/415 ,  C12N9/10 ,  C12N15/54 ,  C12N15/62 ,  G01N33/564 ,  G01N33/68

Abstract: Un antígeno para detectar la enfermedad celíaca que comprende una proteína gliadina desamidada recombinante o sintética que comprende un hexámero de péptidos, en el que cada péptido tiene la secuencia de SEQ ID NO: 1.

公开(公告)号：AU2017272223B2

公开(公告)日：2019-05-09

申请号：AU2017272223

申请日：2017-12-06

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. 9765390_1 (GHMatters) P97180.AU.1

公开(公告)号：AU2017272223A1

公开(公告)日：2018-01-04

申请号：AU2017272223

申请日：2017-12-06

Applicant: BIO-RAD LABORATORIES INC

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. 9765390_1 (GHMatters) P97180.AU.1

公开(公告)号：AU2012348122B2

公开(公告)日：2017-09-14

申请号：AU2012348122

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  G01N33/543

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：AU2012348122A1

公开(公告)日：2014-06-19

申请号：AU2012348122

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：CA2857177A1

公开(公告)日：2013-06-13

申请号：CA2857177

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/564

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

公开(公告)号：EP2788760A4

公开(公告)日：2015-07-15

申请号：EP12855209

申请日：2012-12-03

Applicant: BIO RAD LABORATORIES

Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

IPC: G01N33/53 ,  C07K14/415 ,  C12N9/10 ,  C12N15/54 ,  C12N15/62 ,  G01N33/564 ,  G01N33/68

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06