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    組換え脱アミドグリアジン抗原
    1.
    发明专利
    組換え脱アミドグリアジン抗原 审中-公开

    公开(公告)号：JP2015091878A

    公开(公告)日：2015-05-14

    申请号：JP2015021121

    申请日：2015-02-05

    Applicant: BIO RAD LABORATORIES

    Inventor： WATKINS MICHAEL I ,  GREGORY A MARR ,  YANG XIAOYUN ,  RICHARD BRUEHL ,  SHAN DAMING ,  COLEMAN PATRICK F

    IPC: C07K14/415 ,  C07K19/00 ,  C12N9/10 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/48 ,  G01N33/531 ,  G01N33/543 ,  G01N33/564

    Abstract: 【課題】本発明は、被験者からの体液のサンプルを固相支持体上に固定されたグリアジン融合タンパク質から形成された抗原と接触させることにより、被験者がセリアック病に罹患しているか否かを決定する方法を提供する。本発明はまた、セリアック病を検出する抗原を提供する。【解決手段】セリアック病を検出する抗原は、タグに共有結合した組換え脱アミドグリアジンを含み、グリアジン融合タンパク質を形成する。抗原のグリアジン融合タンパク質は、グルタチオン−Sトランスフェラーゼ(GST)タンパク質等のタグに結合された組換え脱アミドグリアジンを含む。抗原は、タグを通してグリアジン融合タンパク質を固相支持体に固定することにより作製される。抗原は更に、グリアジン融合タンパク質に架橋された組織トランスグルタミナーゼ(tTG)を含むことができる。tTGが存在する場合、固相に固定される前に、tTGと組換え脱アミドグリアジンが混合される。【選択図】図1

    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN
    4.
    发明专利
    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN 未知

    公开(公告)号：AU2013208412A1

    公开(公告)日：2013-08-22

    申请号：AU2013208412

    申请日：2013-07-22

    Applicant: BIO RAD LABORATORIES

    Inventor： WATKINS MICHAEL I ,  MARR GREGORY A ,  YANG XIAOYUN ,  BRUEHL RICHARD ,  SHAN DAMING ,  COLEMAN PATRICK F

    IPC: C07K14/415 ,  A61K38/00 ,  C07H21/04 ,  C12N5/10 ,  C12N15/63 ,  G01N33/53

    Abstract: Abstract The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase. Fig. 1

    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN
    5.
    发明专利
    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN 未知

    公开(公告)号：CA2721582A1

    公开(公告)日：2009-10-29

    申请号：CA2721582

    申请日：2009-04-17

    Applicant: BIO RAD LABORATORIES

    Inventor： WATKINS MICHAEL I ,  MARR GREGORY A ,  YANG XIAOYUN ,  BRUEHL RICHARD ,  SHAN DAMING ,  COLEMAN PATRICK F

    IPC: A61K38/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/10 ,  C12N15/63 ,  G01N33/53

    Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN
    6.
    发明申请
    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN 审中-公开
    重组体灭活的GLIADIN抗原

    公开(公告)号：WO2013085851A3

    公开(公告)日：2014-08-14

    申请号：PCT/US2012067639

    申请日：2012-12-03

    Applicant: BIO RAD LABORATORIES

    Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

    IPC: G01N33/53 ,  G01N33/543

    CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

    Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

    Abstract translation: 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白的六聚体形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过将麦醇溶蛋白融合蛋白固定在固体支持物上来制备抗原。 抗原可以进一步包括与麦醇溶蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。

    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN
    8.
    发明专利
    RECOMBINANT DEAMIDATED GLIADIN ANTIGEN 未知

    公开(公告)号：CA2857177C

    公开(公告)日：2020-06-23

    申请号：CA2857177

    申请日：2012-12-03

    Applicant: BIO RAD LABORATORIES

    Inventor： WALKER ROGER ,  LU YABIN ,  DESAI URVEE ,  SHAN DAMING

    IPC: G01N33/564

    Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

    Recombinant deamidated gliadin antigen
    10.
    发明专利
    Recombinant deamidated gliadin antigen 未知

    公开(公告)号：AU2009239558A1

    公开(公告)日：2009-10-29

    申请号：AU2009239558

    申请日：2009-04-17

    Applicant: BIO RAD LABORATORIES

    Inventor： WATKINS MICHAEL I ,  BRUEHL RICHARD ,  YANG XIAOYUN ,  SHAN DAMING ,  COLEMAN PATRICK F ,  MARR GREGORY A

    IPC: C07K14/415 ,  A61K38/00 ,  C07H21/04 ,  C12N5/10 ,  C12N15/63 ,  G01N33/53

    Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing on the solid support the gliadin fusion protein via the tag. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

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