中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

22 records (took 0.021 seconds)

    ENCAPSULATED IPG STRIPS
    11.
    发明专利
    ENCAPSULATED IPG STRIPS 未知

    公开(公告)号：CA2318542A1

    公开(公告)日：2000-06-02

    申请号：CA2318542

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS ADRIANA J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip (12) from atmospheric exposure and yet contains sufficient volumetric capacity (15) to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures (21, 22) each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures (23) through which lipid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap (24, 25, 26) that is removable to expose the aperture.

    12.
    发明专利
    未知

    公开(公告)号：DE69926725T2

    公开(公告)日：2006-06-29

    申请号：DE69926725

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.

    Encapsulated IPG strips
    13.
    发明专利
    Encapsulated IPG strips 未知

    公开(公告)号：AU762384B2

    公开(公告)日：2003-06-26

    申请号：AU1127900

    申请日：1999-10-20

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  HARBERS ADRIANA J

    IPC: G01N27/447 ,  B01D57/02

    Abstract: Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip from atmospheric exposure and yet contains sufficient volumetric capacity to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures through which liquid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap that is removable to expose the aperture.

    Dry powder formulation for protein labeling
    14.
    发明专利
    Dry powder formulation for protein labeling 未知

    公开(公告)号：AU7814101A

    公开(公告)日：2002-03-04

    申请号：AU7814101

    申请日：2001-08-03

    Applicant: BIO RAD LABORATORIES

    Inventor： ZHU MINGDE ,  OLECH LEE

    IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

    Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by an electrophoretic or chromatographic procedure are coupled to a dye in an unusually efficient manner by combining the sample with a solid dry composition containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The solid and dry form of the composition prevents the dye from deteriorating or decomposing, and the combination of components in the composition allows the dye to couple to the proteins in a relatively uniform manner with no overstaining of the protein when the composition and the sample are heated together and held at an elevated temperature for a short period of time.

    DRY POWDER FORMULATION FOR PROTEIN LABELING
    15.
    发明专利
    DRY POWDER FORMULATION FOR PROTEIN LABELING 未知

    公开(公告)号：CA2420149A1

    公开(公告)日：2002-02-28

    申请号：CA2420149

    申请日：2001-08-03

    Applicant: BIO RAD LABORATORIES

    Inventor： OLECH LEE ,  ZHU MINGDE

    IPC: G01N33/58 ,  G01N21/76 ,  G01N21/78 ,  G01N27/447 ,  G01N33/483 ,  G01N33/543 ,  G01N33/68

    Abstract: The proteins in a biological sample that is sought to be analyzed for its protein composition by a chromatographic procedure are coupled to a dye in a n unusually efficient manner by combining the sample with a dry powdered mixtu re containing the dye, a buffering agent, and in preferred embodiments, a denaturing agent as well. The dry powdered form of the mixture avoids deterioration or decomposition of the dye, and the combination of components in the mixture allows the dye to couple to the proteins in a relatively uniform and highly controllable manner when the powder mixture and sample ar e heated together and held at an elevated temperature until some or all of the powder dissolves.

    CARRIER AMPHOLYTES OF HIGH PH RANGE
    16.
    发明申请
    CARRIER AMPHOLYTES OF HIGH PH RANGE 审中-公开
    高PH范围的载体AMPHOLYTES

    公开(公告)号：WO2005072121A3

    公开(公告)日：2006-04-27

    申请号：PCT/US2005001061

    申请日：2005-01-12

    Applicant: BIO RAD LABORATORIES ,  OLECH LEE

    Inventor： OLECH LEE

    IPC: C07C279/12 ,  G01N27/447 ,  G01N27/26 ,  C07C279/00

    CPC classification number: G01N27/44795 ,  C07C279/12 ,  G01N27/44747

    Abstract: Carboxylic acid-substituted polyalkylene polyamines in which amine nitrogen atoms on the polyamine backbone structure are replaced by guanidine groups provide a pH range extending into high pH values. These substances are useful as carrier ampholytes in isoelectric focusing.

    Abstract translation: 羧胺取代的聚亚烷基多胺，其中多胺主链结构上的胺氮原子被胍基团取代，提供了延伸到高pH值的pH范围。 这些物质可用作等电聚焦中的载体两性电解质。

    ALKYL-SUBSTITUTED VINYL PYRIDINES FOR MASS-CODED TAGGING OF PROTEINS
    17.
    发明申请
    ALKYL-SUBSTITUTED VINYL PYRIDINES FOR MASS-CODED TAGGING OF PROTEINS 审中-公开
    用于蛋白质编码标记的烷基取代的乙烯基吡啶

    公开(公告)号：WO2005024041A3

    公开(公告)日：2005-08-04

    申请号：PCT/US2004029018

    申请日：2004-09-02

    Applicant: BIO RAD LABORATORIES ,  OLECH LEE

    Inventor： OLECH LEE

    IPC: C07K14/47 ,  C12Q20060101 ,  G01N33/00 ,  G01N33/20 ,  G01N33/68

    CPC classification number: G01N33/6848 ,  Y10T436/142222

    Abstract: Comparative studies of proteins from two or more cell populations are performed by alkylating the cysteine thiol groups of the proteins of all populations with vinyl pyridines in which the pyridine ring in at least one of the populations, in addition to the vinyl substituent, bears one or more alkyl substituents, the various populations thus differing in the alkyl substituents on the pyridine ring. Proteins of one population are thus alkylated with vinyl pyridine itself and those of another population with an alkyl-substituted vinyl pyridine, or both with alkyl-substituted vinyl pyridines using different alkyl groups or a different number of alkyl groups. The mass differential afforded by the difference in alkyl substitution affords a means of pairing individual proteins of one population with those of another while differentiating between the two, using analytical techniques disclosed in the prior art for isotope-coded affinity tagging.

    Abstract translation: 来自两个或多个细胞群体的蛋白质的比较研究是通过用乙烯基吡啶烷基化所有群体的蛋白质的半胱氨酸硫醇基团进行的，其中至少一个种群中的吡啶环除了乙烯基取代基之外，还含有一个或 更多的烷基取代基，因此各种群体在吡啶环上的烷基取代基不同。 因此，一个群体的蛋白质由乙烯基吡啶本身和另一个群体与烷基取代的乙烯基吡啶烷基化，或两者使用不同烷基或不同数目的烷基烷基取代的乙烯基吡啶二烷基化。 通过烷基取代的差异提供的质量差异提供了使用现有技术中公开的用于同位素编码的亲和标记的分析技术，将一个群体的单个蛋白质与另一个群体的单个蛋白质与两者之间的差异进行分化的方法。

Patent Agency Ranking