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    MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS
    15.
    发明申请
    MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS 审中-公开
    用主要醇，二醇和酸共同生产异丙酚的微生物和方法

    公开(公告)号：WO2011031897A1

    公开(公告)日：2011-03-17

    申请号：PCT/US2010/048318

    申请日：2010-09-09

    Applicant: GENOMATICA, INC. ,  PHARKYA, Priti ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  BURK, Mark, J. ,  SUN, Jun

    Inventor： PHARKYA, Priti ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  BURK, Mark, J. ,  SUN, Jun

    IPC: C12P7/04 ,  C12N15/74

    CPC classification number: C12P7/40 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18

    Abstract: The invention provides a non-naturally occurring microbial organism having n-propanol and isopropanol pathways, 1,4-butanediol (14-BDO) and isopropanol pathways, 1,3-butanediol (13- BDO) and isopropanol pathways or methylacrylic acid (MAA) and isopropanolpathways. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in each of the respective n-propanol, 14-BDO, 13-BDO or MAA and isopropanol pathways. The invention additionally provides a method for co-producing n-propanol and isopropanol, 14-BDO and isopropanol, 13-BDO and isopropanol or MAA and isopropanol. The method can include culturing an n-propanol and an isopropanol co-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an n-propanol, an isopropanol, a 14-BDO, a 13-BDO and/or a MAA pathway enzyme in a sufficient amount to produce each of the respective products, under conditions and for a sufficient period of time to produce each of the respective products.

    Abstract translation: 本发明提供具有正丙醇和异丙醇途径的非天然存在的微生物生物，1,4-丁二醇（14-BDO）和异丙醇途径，1,3-丁二醇（13-BDO）和异丙醇途径或甲基丙烯酸（MAA ）和异丙醇通道。 微生物生物体含有至少一种外源核酸，其编码在各自的正丙醇，14-BDO，13-BDO或MAA和异丙醇途径中的酶。 本发明另外提供了共同生产正丙醇和异丙醇，14-BDO和异丙醇，13-BDO和异丙醇或MAA和异丙醇的方法。 该方法可以包括培养正丙醇和异丙醇共同生产微生物，其中微生物生物体表达至少一种编码正丙醇，异丙醇，14-BDO，13-BDO和/或 足够量的MAA途径酶，以在产生各个产物的条件下和足够的时间段内产生各个产物。

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID
    16.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID 审中-公开
    丙二酸，十六烷二胺和6-氨基酸的生物合成的微生物和方法

    公开(公告)号：WO2010129936A1

    公开(公告)日：2010-11-11

    申请号：PCT/US2010/034144

    申请日：2010-05-07

    Applicant: GENOMATICA, INC. ,  BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

    Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

    IPC: C12N1/21 ,  C12P13/04 ,  C12P7/44

    CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

    Abstract: The invention provides a non-naturally occurring microbial organism having a 6- aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

    Abstract translation: 本发明提供了具有6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径的非天然存在的微生物。 微生物生物体含有至少一种编码各自的6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径中的酶的外源核酸。 本发明另外提供了制备6-氨基己酸，己内酰胺，己二胺或乙酰丙酸的方法。 该方法可以包括培养6-氨基己酸，己内酰胺或六亚甲基二胺生产微生物，其中微生物生物表达至少一种编码6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径酶的外源核酸，其量足以产生 各自的产物在条件下和足够的时间内产生6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸。

    MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID
    17.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID 审中-公开

    公开(公告)号：WO2009135074A3

    公开(公告)日：2009-11-05

    申请号：PCT/US2009/042426

    申请日：2009-04-30

    Applicant: GENOMATICA, INC. ,  BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

    Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

    IPC: C12P7/42 ,  C12N1/20

    Abstract: The invention provides a non-naturally occurring microbial organism having a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3- hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

    COMPOSITIONS AND MULTIPLEXED SYSTEMS FOR COUPLED CELL-FREE TRANSCRIPTION-TRANSLATION AND PROTEIN SYNTHESIS AND METHODS FOR USING THEM
    19.
    发明申请
    COMPOSITIONS AND MULTIPLEXED SYSTEMS FOR COUPLED CELL-FREE TRANSCRIPTION-TRANSLATION AND PROTEIN SYNTHESIS AND METHODS FOR USING THEM 审中-公开
    用于联合细胞免疫转录和蛋白质合成的组合物和多重系统及其使用方法

    公开(公告)号：WO2017031399A1

    公开(公告)日：2017-02-23

    申请号：PCT/US2016/047704

    申请日：2016-08-19

    Applicant: GENOMATICA, INC.

    Inventor： CULLER, Stephanie ,  CHEN, Ihsiung Brandon ,  PHARKYA, Priti ,  VAN DIEN, Steve ,  BARTON, Nelson

    IPC: A61K35/12 ,  A61K48/00 ,  C12N5/00 ,  C12N5/02 ,  C12N15/09 ,  C12N5/10

    CPC classification number: A61K35/66 ,  Y02A50/409 ,  Y02A50/469 ,  A61K35/12

    Abstract: In alternative embodiments, provided herein are transcription/ translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the combinatorial biosynthesis of natural products (NPs), natural product analogs (NPAs) and secondary metabolites. In alternative embodiments, exemplary TX-TL systems as provided herein are used for the rapid prototyping of complex biosynthetic pathways as a way to rapidly assess combinatorial and biosynthetic designs before moving to cellular hosts. In alternative embodiments, these exemplary TX-TL systems are multiplexed for high-throughput (HT) automation and for prototyping engineered platforms for the synthesis or modification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites analogs.

    Abstract translation: 在替代实施方案中，本文提供的是转录/翻译（TX-TL）系统和使用它们作为用于合成，修饰和鉴定天然产物（NP）和天然产物类似物（NPA）和次要的快速原型平台的方法。 代谢物，来自生物合成基因簇管线。 在替代实施方案中，本文提供的示例性TX-TL系统用于天然产物（NP），天然产物类似物（NPA）和次级代谢物的组合生物合成。 在替代实施例中，本文提供的示例性TX-TL系统用于复杂生物合成途径的快速原型化，作为在移动到蜂窝主机之前快速评估组合和生物合成设计的方式。 在替代实施例中，这些示例性TX-TL系统被复用用于高通量（HT）自动化和用于天然产物（NP）和天然产物类似物（NPAs）和次级代谢物类似物的合成或修饰的原型设计平台。

    NON-NATURAL MICROBIAL ORGANISMS WITH IMPROVED ENERGETIC EFFICIENCY
    20.
    发明申请
    NON-NATURAL MICROBIAL ORGANISMS WITH IMPROVED ENERGETIC EFFICIENCY 审中-公开
    具有改善能源效率的非天然微生物有机体

    公开(公告)号：WO2016044713A1

    公开(公告)日：2016-03-24

    申请号：PCT/US2015/050923

    申请日：2015-09-18

    Applicant: GENOMATICA, INC.

    Inventor： PHARKYA, Priti ,  BURGARD, Anthony P. ,  NUNEZ VAN NAME, Eric Roland

    IPC: C12P7/42 ,  C12P7/16

    CPC classification number: C12N15/52 ,  C12N9/1205 ,  C12N9/88 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12Y207/0104 ,  C12Y401/02009 ,  C12Y401/02022 ,  Y02E50/343

    Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.

    Abstract translation: 本发明提供了含有酶途径和/或代谢修饰用于增强通过乙酰辅酶A，或草酰乙酸和乙酰辅酶A的碳通量的非天然微生物生物。 本发明的实施方案包括具有乙酰辅酶A途径和包含磷酸酮醇酶（PK途径）的草酰乙酸的途径的微生物。 生物体还具有（i）增强非磷酸转移酶系统（非PTS）对于糖吸收的活性的遗传修饰，和/或（ii）对生物体的电子传递链（ETC）的遗传修饰 ），其提高了ATP生产的效率，这增强了减少等效物或两者的可用性。 微生物生物可以任选地包括（iii）维持，减弱或消除磷酸转移酶系统（PTS）用于糖摄取的活性的遗传修饰。 通过乙酰辅酶A和草酰乙酸酯的增强的碳通量可用于生产生物活性化合物，并且微生物有机体可以进一步包括能够生产生物活性化合物的途径。

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