公开(公告)号：AU2009228012A1

公开(公告)日：2009-10-01

申请号：AU2009228012

申请日：2009-03-27

Applicant: HARVARD COLLEGE

Inventor： PHILLIPS SCOTT T ,  WHITESIDES GEORGE M ,  DICKEY MICHAEL D ,  ROZKIEWICZ DOROTA ,  WILEY BENJAMIN ,  SIEGEL ADAM C ,  MARTINEZ ANDRES W

IPC: G01N35/00 ,  G01N27/26 ,  G01N33/48

公开(公告)号：CA2754577C

公开(公告)日：2018-07-10

申请号：CA2754577

申请日：2010-03-08

Applicant: HARVARD COLLEGE

Inventor： NIE ZHIHONG ,  NIJHUIS CHRISTIAN ,  CHEN XIN ,  MARTINEZ ANDRES W ,  NAROVLYANSKY MAX ,  WHITESIDES GEORGE M

IPC: B01L3/00

Abstract: Microfluidic, electrochemical devices are described. The microfluidic, electrochemical device comprises one or more electrode(s) on a substrate and a patterned porous, hydrophilic layer having a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic channels within the patterned porous, hydrophilic layer, wherein the hydrophilic channel(s) comprises a hydrophilic region which is in fluidic commu-nication with the electrode(s). In some embodiments, the electrodes comprise a working electrode, a counter electrode, and a ref-erence electrode. In some embodiments, the microfluidic, electrochemical device further comprises a fluid sink. The method of as-sembling the microfluidic, electrochemical device is described. The method of using the device for electrochemical analysis of one or more analytes is also described.

公开(公告)号：CA2667702C

公开(公告)日：2016-06-14

申请号：CA2667702

申请日：2007-10-18

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  PHILLIPS SCOTT T ,  MARTINEZ ANDRES W ,  BUTTE MANISH J ,  WONG AMY ,  THOMAS SAMUEL ,  SINDI HAYAT ,  VELLA SARAH JANE ,  CARRILHO EMANUEL ,  MIRICA KATHERINE A ,  LIU YANYAN

IPC: G01N33/558

Abstract: Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.

公开(公告)号：AU2011212916B2

公开(公告)日：2015-07-02

申请号：AU2011212916

申请日：2011-02-03

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  MIRICA KATHERINE A ,  MARTINEZ ANDRES W ,  CHENG CHAO-MIN ,  PHILLIPS SCOTT T ,  MASCARENAS MONICA ,  LIU XINYU ,  LI XIUJUN

IPC: B01L3/00

Abstract: The disclosure provides low cost, portable three-dimensional devices for performing multiplexed assays. The devices comprise at least two substantially planar layers disposed in parallel planes, wherein one of the layers is movable relative to each other parallel to the planes to permit the establishment of fluid flow communication serially between the two layers.

公开(公告)号：AU2007310987B2

公开(公告)日：2014-01-09

申请号：AU2007310987

申请日：2007-10-18

Applicant: HARVARD COLLEGE

Inventor： VELLA SARAH JANE ,  MARTINEZ ANDRES W ,  WONG AMY ,  MIRICA KATHERINE A ,  WHITESIDES GEORGE M ,  BUTTE MANISH J ,  SINDI HAYAT ,  PHILLIPS SCOTT T ,  CARRILHO EMANUEL ,  THOMAS SAMUEL ,  LIU YANYAN

IPC: G01N33/558

Abstract: Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.

公开(公告)号：BRPI0718473A2

公开(公告)日：2013-12-03

申请号：BRPI0718473

申请日：2007-10-18

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  PHILLIPS SCOTT T ,  MARTINEZ ANDRES W ,  BUTTE MANISH J ,  WONG AMY ,  THOMAS SAMUEL ,  SINDI HAYAT ,  VELLA SARAH JANE ,  MIRICA KATHERINE A ,  LIU YANYAN ,  CARRILHO EMANUEL

IPC: G01N33/558

公开(公告)号：AU2011212916A1

公开(公告)日：2012-08-16

申请号：AU2011212916

申请日：2011-02-03

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  MIRICA KATHERINE A ,  MARTINEZ ANDRES W ,  CHENG CHAO-MIN ,  PHILLIPS SCOTT T ,  MASCARENAS MONICA ,  LIU XINYU ,  LI XIUJUN

IPC: B01L3/00

Abstract: The disclosure provides low cost, portable three-dimensional devices for performing multiplexed assays. The devices comprise at least two substantially planar layers disposed in parallel planes, wherein one of the layers is movable relative to each other parallel to the planes to permit the establishment of fluid flow communication serially between the two layers.

公开(公告)号：AU2010221117A1

公开(公告)日：2011-09-29

申请号：AU2010221117

申请日：2010-03-08

Applicant: HARVARD COLLEGE

Inventor： EMANUEL CARRILHO ,  MARTINEZ ANDRES W ,  WHITESIDES GEORGE M

IPC: B01L3/00

Abstract: Methods of patterning hydrophobic regions onto hydrophilic substrates are described.

公开(公告)号：AU2010221102A1

公开(公告)日：2011-09-29

申请号：AU2010221102

申请日：2010-03-08

Applicant: HARVARD COLLEGE

Inventor： ZHIHONG NEI ,  CHRISTIAN NIJHUIS ,  XIN CHEN ,  MARTINEZ ANDRES W ,  MAX NAROVLYANSKY ,  WHITESIDES GEORGE M

IPC: B01L3/00

Abstract: Microfluidic, electrochemical devices are described. The microfluidic, electrochemical device comprises one or more electrode(s) on a substrate and a patterned porous, hydrophilic layer having a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic channels within the patterned porous, hydrophilic layer, wherein the hydrophilic channel(s) comprises a hydrophilic region which is in fiuidic communication with the electrode(s). In some embodiments, the electrodes comprise a working electrode, a counter electrode, and a reference electrode. In some embodiments, the microfluidic, electrochemical device further comprises a fluid sink. The method of assembling the microfluidic, electrochemical device is described. The method of using the device for electrochemical analysis of one or more analytes is also described.

公开(公告)号：WO2009121037A3

公开(公告)日：2009-12-17

申请号：PCT/US2009038693

申请日：2009-03-27

Applicant: HARVARD COLLEGE ,  CARRILHO EMANUEL ,  MARTINEZ ANDRES W ,  MIRICA KATHERINE A ,  PHILLIPS SCOTT T ,  SIEGEL ADAM C ,  WILEY BENJAMIN ,  WHITESIDES GEORGE M

Inventor： CARRILHO EMANUEL ,  MARTINEZ ANDRES W ,  MIRICA KATHERINE A ,  PHILLIPS SCOTT T ,  SIEGEL ADAM C ,  WILEY BENJAMIN ,  WHITESIDES GEORGE M

IPC: G01N35/00 ,  G01N33/48

CPC classification number: B01L3/502707 ,  B01L3/5023 ,  B01L3/502738 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/126 ,  B01L2300/1855 ,  B01L2300/1877 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0672 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0025 ,  F16K2099/008

Abstract: Three-dimensional microfluidic devices including by a plurality of patterned porous, hydrophilic layers and a fluid-impermeable layer disposed between every two adjacent patterned porous, hydrophilic layers are described. Each patterned porous, hydrophilic layer has a fluid-impermeable barrier which substantially permeates the thickness of the porous, hydrophilic layer and defines boundaries of one or more hydrophilic regions within the patterned porous, hydrophilic layer. The fluid-impermeable layer has openings which are aligned with at least part of the hydrophilic region within at least one adjacent patterned porous, hydrophilic layer. Microfluidic assay device, microfluidic mixer, microfluidic flow control device are also described.

Abstract translation: 描述了包括由多个图案化的多孔亲水层和设置在每两个相邻的图案化多孔亲水层之间的流体不可渗透层的三维微流体装置。 每个图案化的多孔亲水层具有流体不可渗透的屏障，其基本上渗透多孔亲水层的厚度并限定图案化多孔亲水层内的一个或多个亲水区域的边界。 不透流体层具有与至少一个相邻图案化多孔亲水层内的亲水区域的至少一部分对齐的开口。 还描述了微流体测定装置，微流体混合器，微流体控制装置。