公开(公告)号：CA2564609C

公开(公告)日：2014-02-11

申请号：CA2564609

申请日：2004-12-29

Applicant: HARVARD COLLEGE

Inventor： SIA SAMUEL K ,  LINDER VINCENT ,  PARVIZ BABAK A ,  SIEGEL ADAM ,  WHITESIDES GEORGE M

IPC: G01N21/53 ,  B01L3/00 ,  G01N21/05 ,  G01N21/59 ,  G01N33/53 ,  G01N33/58

Abstract: An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion (140) of a surface (130) of a microfluidic chamber (120,122,124); passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

公开(公告)号：AU2005209321B2

公开(公告)日：2010-11-18

申请号：AU2005209321

申请日：2005-01-26

Applicant: HARVARD COLLEGE

Inventor： LINDER VINCENT ,  SIA SAMUEL K ,  WHITESIDES GEORGE M

IPC: B01J4/02 ,  B01L3/00

Abstract: Method of determining an analyte in a fluid sample, such as whole blood, urine, or saliva, comprising: providing a metal colloid; passing the fluid sample over a surface of a microfluidic channel having disposed thereon a binding partner for the analyte to form an analyte-binding partner complex having the metal colloid associated therewith; flowing a metal precursor solution, such as a silver solution, over the surface to form a metallic layer; flowing in series a predetermined sequence of fluid plugs through the microchannel including first, second and third fluids, wherein the first and second fluids, such as the metal precursor solution and a rinse solution, are separated by the third fluid, for example air, which is immiscible with the first and second fluids; and determining the metallic layer by determining the transmission of light through the layer.

公开(公告)号：AU2001264960B2

公开(公告)日：2006-06-08

申请号：AU2001264960

申请日：2001-05-25

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  CHERNIAVSKAYA OKSANA ,  CHIU DANIEL T ,  MCDONALD COOPER ,  ANDERSON JANELLE R ,  JACKMAN REBECCA J

IPC: B01L3/00 ,  G01N37/00 ,  B01F13/00 ,  B01L3/02 ,  B29C31/04 ,  B29C33/40 ,  B29C33/42 ,  B29C39/02 ,  B29C39/24 ,  B29C65/00 ,  B81B1/00 ,  B81C1/00 ,  B82B3/00 ,  C12M1/34

Abstract: The present invention provides, in certain embodiments, improved microfluidic systems and methods for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The inventive methods can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems provided according to the invention can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks of the invention can be fabricated via replica molding processes, also provided by the invention, utilizing mold masters including surfaces having topological features formed by photolithography. The microfluidic networks of the invention are, in some cases, comprised of a single replica molded layer, and, in other cases, are comprised of two, three, or more replica molded layers that have been assembled to form the overall microfluidic network structure. The present invention also describes various novel applications for using the microfluidic network structures provided by the invention.

公开(公告)号：AU2005247040A1

公开(公告)日：2006-02-02

申请号：AU2005247040

申请日：2005-12-23

Applicant: HARVARD COLLEGE

Inventor： ARIAS FRANCISCO ,  WHITESIDES GEORGE M ,  KENIS PAUL J ,  ISMAGILOV RUSTEM F ,  DENG TAO

IPC: B31C1/00 ,  H05K3/00

公开(公告)号：AU2001243656B2

公开(公告)日：2005-09-29

申请号：AU2001243656

申请日：2001-03-15

Applicant: HARVARD COLLEGE

Inventor： JACKMAN REBECCA J ,  KANE RAVI ,  OSTUNI EMANUELE ,  WHITESIDES GEORGE M ,  DUFFY DAVID C

IPC: G01N33/48 ,  B01J19/00 ,  C12N1/00 ,  C12N11/06 ,  C40B60/14 ,  B01J019/00

Abstract: The present invention provides a masking system for selectively applying cells to predetermined regions of a surface. A mask is positioned adjacent to a surface to cover some portions of the surface while allowing other portions of the surface to remain uncovered. Cells then are applied to uncovered portions of the surface and the mask removed. Alternatively, a cell-adhesion promoter is applied to uncovered portions of the surface, and then cells are applied to the surface before or after removal of the mask from the surface. The masking system can be pre-coated, at least on those surfaces which will come into contact with cells, with a cell-adhesion inhibitor to resist absorption of cells and thereby avoid cell damage when the mask is removed (if cells are deposited prior to removal of the mask). A polymeric elastomeric mask that comes into cohesive-conformal contact with a surface to be patterned can be used.

公开(公告)号：CA2564211A1

公开(公告)日：2005-08-11

申请号：CA2564211

申请日：2005-01-26

Applicant: HARVARD COLLEGE

Inventor： WHITESIDES GEORGE M ,  SIA SAMUEL K ,  LINDER VINCENT

IPC: B01J4/02 ,  B01L3/00

Abstract: Method and device for storing and/or delivering fluids, wherein at least a first and a second fluid, such as chemical or biochemical reagents or rinse solutions, are maintained separately from each other in a common vessel and transferred in series from the vessel to a reaction site to carry out a predetermined chemical or biochemical reaction. Separation may be achieved b y interposing a third fluid, e.g., a gaseous fluid plug, between the first and second fluids.

公开(公告)号：AU2003253751A1

公开(公告)日：2004-01-19

申请号：AU2003253751

申请日：2003-06-30

Applicant: HARVARD COLLEGE

Inventor： STONE HOWARD A ,  ANNA SHELLY L ,  BONTOUX NATHALIE ,  LINK DARREN ROY ,  WEITZ DAVID A ,  GITLIN IRINA ,  GARSTECKI PIOTR ,  DILUZIO WILLOW ,  WHITESIDES GEORGE M ,  KUMACHEVA EUGENIA

IPC: B01F25/46 ,  B01L3/00 ,  B05B7/04 ,  B01F13/00

Abstract: A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-availabe, inexpensive material using simple techniques.

公开(公告)号：AU2003230860A8

公开(公告)日：2003-10-27

申请号：AU2003230860

申请日：2003-04-10

Applicant: HARVARD COLLEGE

Inventor： JACOBS HEIKO O ,  SCHWARTZ ALEXANDER ,  GRACIAS DAVID H ,  TAO ANDREA R ,  WHITESIDES GEORGE M

IPC: H01L21/60 ,  H01L21/98 ,  H01L23/538 ,  H05K1/18 ,  H05K3/34 ,  H01L21/469

Abstract: The present invention is directed to self-assembly of structures, and, in particular, to self-assembly by action of a liquid patterned onto a substrate. In one embodiment, the present invention is directed to method of self-assembling a plurality of structures. The method includes patterning a first liquid onto a first substrate, and, while at least a portion of the first liquid remains in liquid form, self-assembling at least a portion of the plurality of structures onto the substrate by action of the first liquid and according to the pattern of the first liquid. In another embodiment, the present invention is directed to a display. The display includes a first substrate and a first plurality of electrical connectors being in electrical connection with one another. The display further includes a second substrate, at least some of the second plurality of electrical connectors being in electrical connection with one another. The display further includes a plurality of display elements each electrically connected to at least one of the first plurality of electrical connectors and the second plurality of connectors. In this embodiment, each of the plurality of display elements has an average diameter of less than 1 millimeter.

公开(公告)号：AU9278701A

公开(公告)日：2002-03-26

申请号：AU9278701

申请日：2001-09-18

Applicant: HARVARD COLLEGE

Inventor： TAKAYAMA SHUICHI ,  OSTUNI EMANUELE ,  LEDUC PHILIP ,  NARUSE KEIJI ,  INGBER DONALD E ,  WHITESIDES GEORGE M

IPC: G01N37/00 ,  A61K35/12 ,  B81B1/00 ,  C12N5/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/52

Abstract: The present invention is directed, in certain embodiments, to improved, small scale systems and methods able to selectively treat parts of a single cell, including, in certain embodiments, portions of a main body portion of a single cell, and able, in certain embodiments, to establish long-term gradients of active substances within subcellular regions of a single cell. The present invention provides, in some embodiments, techniques for selectively contacting a portion of the surface of a biological cell with a fluid or fluid component carrying a particular potential for a biophysical or biochemical interaction with the cell, and simultaneously contacting a different portion of the surface of the cell with another fluid or fluid component having a different potential for the biophysical or biochemical interaction with the cell.

公开(公告)号：CA2422762A1

公开(公告)日：2002-03-21

申请号：CA2422762

申请日：2001-09-18

Applicant: HARVARD COLLEGE ,  CHILDRENS MEDICAL CENTER

Inventor： OSTUNI EMANUELE ,  NARUSE KEIJI ,  INGBER DONALD E ,  LEDUC PHILIP ,  WHITESIDES GEORGE M ,  TAKAYAMA SHUICHI

IPC: G01N37/00 ,  A61K35/12 ,  B81B1/00 ,  C12N5/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/52 ,  C12Q1/02 ,  C12N1/38

Abstract: The present invention is directed, in certain embodiments, to improved, smal l scale systems and methods able to selectively treat parts of a single cell, including, in certain embodiments, portions of a main body portion of a sing le cell, and able, in certain embodiments, to establish long-term gradients of active substances within subcellular regions of a single cell. The present invention provides, in some embodiments, techniques for selectively contacti ng a portion of the surface of a biological cell with a fluid or fluid componen t carrying a particular potential for a physiological or biochemical interacti on with the cell, and simultaneously contacting a different portion of the surface of the cell with another fluid or fluid component having a different portion of the surface of the cell with another fluid or fluid component having a different potential for the biophysical or biochemical interaction with the cell.