-
公开(公告)号:DE10137158B4
公开(公告)日:2005-08-04
申请号:DE10137158
申请日:2001-07-30
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , HOFFMANN JUERGEN
Abstract: A method for scanning microscopy is disclosed. It contains the step of generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm; the choosing of a second wavelength spectrum that is arranged spectrally within the first wavelength spectrum; the step of selecting the light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component; and the step of illuminating a specimen with the illuminating light beam. A scanning microscope is also disclosed.
-
公开(公告)号:DE10012462B4
公开(公告)日:2004-07-08
申请号:DE10012462
申请日:2000-03-15
Applicant: LEICA MICROSYSTEMS
Inventor: HOFFMANN JUERGEN
Abstract: An apparatus for illuminating a specimen (1), preferably in confocal fluorescence scanning microscopy is disclosed. Two illumination beam paths (2, 4) are combined with a beam splitter (6) in order to simplify alignment and reduce the optical components in the illumination beam path. The at least one optical component (7) is arranged at least in one of the illumination beam paths (2, 4) and modifies the light; and that the optical properties of the component (7) can be influenced or modified in such a way that the illumination pattern of the illumination beam path (2, 4) in the specimen region changes shape.
-
公开(公告)号:DE10251151A1
公开(公告)日:2004-05-13
申请号:DE10251151
申请日:2002-10-31
Applicant: LEICA MICROSYSTEMS
Inventor: REICHERT WERNER , HOFFMANN JUERGEN
Abstract: The device has a laser light source (2) emitting several wavelengths, an approximately continuously adjustable spectrally selective element (4), an approximately continuously adjustable spectral detector (9), illumination and detection light paths (23,19) and a controller (21) that tunes the selection and detection elements to each other so that a detection signal can be maximized. AN Independent claim is also included for the following: (a) a method of forming an image of a luminous object.
-
公开(公告)号:DE10031458B4
公开(公告)日:2004-03-11
申请号:DE10031458
申请日:2000-06-28
Applicant: LEICA MICROSYSTEMS
Inventor: HOFFMANN JUERGEN
IPC: G02B21/00
Abstract: A scanning microscope (100) possesses at least one illumination source (4), an objective (10), and at least one detector (12). An optical circulator (14) is arranged between the at least one illumination source (4), the objective (10), and the at least one detector (12). In a further embodiment, the scanning microscope (100) is configured as a confocal scanning microscope.
-
公开(公告)号:DE10137158A1
公开(公告)日:2003-02-20
申请号:DE10137158
申请日:2001-07-30
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , HOFFMANN JUERGEN
Abstract: A method for scanning microscopy is disclosed. It contains the step of generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm; the choosing of a second wavelength spectrum that is arranged spectrally within the first wavelength spectrum; the step of selecting the light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component; and the step of illuminating a specimen with the illuminating light beam. A scanning microscope is also disclosed.
-
公开(公告)号:DE10063276C2
公开(公告)日:2002-11-07
申请号:DE10063276
申请日:2000-12-19
Applicant: LEICA MICROSYSTEMS
Inventor: HOFFMANN JUERGEN
Abstract: The scanning microscope includes an illumination beam path, microscope optics and at least one light source which generates an excitation light beam of a first wavelength and a second light beam of a second wavelength. Microscope optics are provided for focussing the excitation light beam onto a first focal region in a first plane of a sample and for focusing the second light beam onto a second focal region in a second plane of the sample. The first focal region and the second focal region overlap partially. The optical properties of the components arranged in the illumination beam path are matched to one another such that optical aberrations are corrected in such a way that the focal regions remain static relative to one another irrespective of the scanning movement.
-
公开(公告)号:DE10044308A1
公开(公告)日:2002-03-21
申请号:DE10044308
申请日:2000-09-07
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , HOFFMANN JUERGEN
Abstract: A method for detection of fluorescent light in scanning microscopy includes dividing an illuminating light beam into a plurality of partial illuminating beams so as to illuminate multiple specimen regions simultaneously. Fluorescing materials in a specimen region are excited via multi-photon excitation. Operating parameters of the light source are adapted for optimum fluorescent photon yield to the properties of the fluorescing material in the specimen region. Fluorescent light of the specimen regions is detected simultaneously.
-
公开(公告)号:DE10027323A1
公开(公告)日:2001-12-06
申请号:DE10027323
申请日:2000-06-05
Applicant: LEICA MICROSYSTEMS
Inventor: KNEBEL WERNER , HOFFMANN JUERGEN
Abstract: An object model (2) is scanned with a beam of light (3) from a source of light (4). Light is detected while reflected from the object model (5), which is scanned with high resolution and accuracy so that an object (1) can be generated from the object model to represent the object model accurately.
-
公开(公告)号:DE10016377A1
公开(公告)日:2001-10-18
申请号:DE10016377
申请日:2000-04-04
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , HOFFMANN JUERGEN , STORZ RAFAEL , ULRICH HEINRICH , BEWERSDORF JOERG , BIRK HOLGER
Abstract: The present invention concerns an apparatus for combining light from at least two laser light sources, preferably in the context of confocal scanning microscopy, and in order to make laser light sources of low output power usable as light sources, in particular for confocal scanning microscopy, is characterized in that the light from the laser light sources has at least approximately the same wavelength; and that at least one beam combining unit that combines the light beams in at least largely lossless fashion is provided.
-
公开(公告)号:DE10006800A1
公开(公告)日:2001-08-16
申请号:DE10006800
申请日:2000-02-15
Applicant: LEICA MICROSYSTEMS
Inventor: ENGELHARDT JOHANN , HAY BILL , HOFFMANN JUERGEN
Abstract: The system is designed so that in the fanned out light beam (1), an optical component part (4) is arranged reflecting and-or refracting the light beam to a detector (3), the optical effective region of which is reduced or increased along the surface, so that by alignment of the component part (4) to the focal line (2) and the resulting covering of the focal line, and the surface of the spectral region arriving at the detector (3) is definable.
-
-
-
-
-
-
-
-
-