11.
    发明专利
    未知

    公开(公告)号:DE10137158B4

    公开(公告)日:2005-08-04

    申请号:DE10137158

    申请日:2001-07-30

    Abstract: A method for scanning microscopy is disclosed. It contains the step of generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm; the choosing of a second wavelength spectrum that is arranged spectrally within the first wavelength spectrum; the step of selecting the light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component; and the step of illuminating a specimen with the illuminating light beam. A scanning microscope is also disclosed.

    12.
    发明专利
    未知

    公开(公告)号:DE10012462B4

    公开(公告)日:2004-07-08

    申请号:DE10012462

    申请日:2000-03-15

    Inventor: HOFFMANN JUERGEN

    Abstract: An apparatus for illuminating a specimen (1), preferably in confocal fluorescence scanning microscopy is disclosed. Two illumination beam paths (2, 4) are combined with a beam splitter (6) in order to simplify alignment and reduce the optical components in the illumination beam path. The at least one optical component (7) is arranged at least in one of the illumination beam paths (2, 4) and modifies the light; and that the optical properties of the component (7) can be influenced or modified in such a way that the illumination pattern of the illumination beam path (2, 4) in the specimen region changes shape.

    14.
    发明专利
    未知

    公开(公告)号:DE10031458B4

    公开(公告)日:2004-03-11

    申请号:DE10031458

    申请日:2000-06-28

    Inventor: HOFFMANN JUERGEN

    Abstract: A scanning microscope (100) possesses at least one illumination source (4), an objective (10), and at least one detector (12). An optical circulator (14) is arranged between the at least one illumination source (4), the objective (10), and the at least one detector (12). In a further embodiment, the scanning microscope (100) is configured as a confocal scanning microscope.

    15.
    发明专利
    未知

    公开(公告)号:DE10137158A1

    公开(公告)日:2003-02-20

    申请号:DE10137158

    申请日:2001-07-30

    Abstract: A method for scanning microscopy is disclosed. It contains the step of generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm; the choosing of a second wavelength spectrum that is arranged spectrally within the first wavelength spectrum; the step of selecting the light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component; and the step of illuminating a specimen with the illuminating light beam. A scanning microscope is also disclosed.

    16.
    发明专利
    未知

    公开(公告)号:DE10063276C2

    公开(公告)日:2002-11-07

    申请号:DE10063276

    申请日:2000-12-19

    Inventor: HOFFMANN JUERGEN

    Abstract: The scanning microscope includes an illumination beam path, microscope optics and at least one light source which generates an excitation light beam of a first wavelength and a second light beam of a second wavelength. Microscope optics are provided for focussing the excitation light beam onto a first focal region in a first plane of a sample and for focusing the second light beam onto a second focal region in a second plane of the sample. The first focal region and the second focal region overlap partially. The optical properties of the components arranged in the illumination beam path are matched to one another such that optical aberrations are corrected in such a way that the focal regions remain static relative to one another irrespective of the scanning movement.

    17.
    发明专利
    未知

    公开(公告)号:DE10044308A1

    公开(公告)日:2002-03-21

    申请号:DE10044308

    申请日:2000-09-07

    Abstract: A method for detection of fluorescent light in scanning microscopy includes dividing an illuminating light beam into a plurality of partial illuminating beams so as to illuminate multiple specimen regions simultaneously. Fluorescing materials in a specimen region are excited via multi-photon excitation. Operating parameters of the light source are adapted for optimum fluorescent photon yield to the properties of the fluorescing material in the specimen region. Fluorescent light of the specimen regions is detected simultaneously.

    20.
    发明专利
    未知

    公开(公告)号:DE10006800A1

    公开(公告)日:2001-08-16

    申请号:DE10006800

    申请日:2000-02-15

    Abstract: The system is designed so that in the fanned out light beam (1), an optical component part (4) is arranged reflecting and-or refracting the light beam to a detector (3), the optical effective region of which is reduced or increased along the surface, so that by alignment of the component part (4) to the focal line (2) and the resulting covering of the focal line, and the surface of the spectral region arriving at the detector (3) is definable.

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