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    FLUOROGENIC DYES FOR HIGH SENSITIVITY DNA DETECTION
    13.
    发明申请
    FLUOROGENIC DYES FOR HIGH SENSITIVITY DNA DETECTION 审中-公开
    荧光染料用于高灵敏度DNA检测

    公开(公告)号：WO2018085449A2

    公开(公告)日：2018-05-11

    申请号：PCT/US2017/059594

    申请日：2017-11-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： GEE, Kyle ,  YANG, Jongtae

    IPC: C07D417/12 ,  C09B23/04 ,  C12Q1/6818

    CPC classification number: C07D417/12 ,  C09B23/04 ,  C12Q1/6818

    Abstract: The present disclosure is directed to unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a quinolinium ring that contains a heteroatom. Compounds of formula (I) are provided herein. The compounds can be useful for fluorescent detection or quantification of nucleic acids. Related methods, uses, and kits are disclosed.

    Abstract translation: 本发明涉及包含通过次甲基桥连接至含有杂原子的喹啉鎓环的取代的吲哚鎓环系统的不对称花青染料。 式（I）的化合物在本文提供。 该化合物可用于荧光检测或核酸定量。 披露了相关的方法，用途和试剂盒。

    METHODS AND COMPOSITIONS FOR DETECTING OR MEASURING CASPASES OR APOPTOSIS
    14.
    发明申请
    METHODS AND COMPOSITIONS FOR DETECTING OR MEASURING CASPASES OR APOPTOSIS 审中-公开
    方法和组合物检测或测量CASPASES或APOPTOSIS

    公开(公告)号：WO2017223147A1

    公开(公告)日：2017-12-28

    申请号：PCT/US2017/038444

    申请日：2017-06-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： GEE, Kyle ,  ZHOU, Wenjun ,  HUANG, Shih-Jung ,  TOMASZEWSKI, Monica

    IPC: C12Q1/48 ,  G01N21/64 ,  G01N33/58

    Abstract: Provided herein are compounds, enzyme substrates, compositions, kits, uses, and methods for detecting the presence or absence of a caspase enzyme, measuring the activity of a caspase enzyme, or detecting the presence or absence of apoptosis. The detection or measurement can occur through intracellular cleavage of a compound or enzyme substrate, which can lead to an increase in fluorescence, e.g., in the violet or red channel, through liberation of a nucleic acid binding dye from a peptide, such as liberation of a DNA-binding dye from a negatively charged peptide comprising a sequence recognized and cleaved by a caspase.

    Abstract translation: 本文提供了用于检测半胱天冬酶存在或不存在，测量半胱天冬酶活性或检测半胱天冬酶的存在或不存在的化合物，酶底物，组合物，试剂盒，用途和方法 细胞凋亡。 检测或测量可通过细胞内裂解化合物或酶底物而发生，所述化合物或酶底物可通过从肽中释放核酸结合染料而导致例如在紫色或红色通道中的荧光增加，例如释放 来自带负电荷的肽的DNA结合染料，其包含被半胱天冬酶识别和切割的序列。

    MODIFIED NUCLEIC ACID BINDING CYANINE DYES FOR THE DETECTION OF REACTIVE OXYGEN SPECIES
    17.
    发明申请
    MODIFIED NUCLEIC ACID BINDING CYANINE DYES FOR THE DETECTION OF REACTIVE OXYGEN SPECIES 审中-公开
    用于检测反应性氧物种的改性核酸结合氰胺染料

    公开(公告)号：WO2013148038A1

    公开(公告)日：2013-10-03

    申请号：PCT/US2013/027805

    申请日：2013-02-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： HU, Yi-Zhen ,  CHEN, Aimei ,  KANG, Hee Chol ,  GEE, Kyle ,  MANDAVILLI, Bhaskar

    IPC: C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/533 ,  G01N33/58

    CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

    Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo . Also described herein are processes for preparing novel reduced dyes, i.e ., ROS probes, for use in the disclosed compositions, methods and kits.

    Abstract translation: 本文公开了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原性核酸结合花青染料，其染料是用于检测体内和/或体内细胞中ROS和测量氧化应激的探针。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

    MODIFIED HYDROCYANINE DYES FOR THE DETECTION OF REACTIVE OXYGEN SPECIES
    19.
    发明申请
    MODIFIED HYDROCYANINE DYES FOR THE DETECTION OF REACTIVE OXYGEN SPECIES 审中-公开
    用于检测反应性氧化物种的改性的氢氯仿染料

    公开(公告)号：WO2012061403A1

    公开(公告)日：2012-05-10

    申请号：PCT/US2011/058806

    申请日：2011-11-01

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  KANG, Hee Chol ,  GEE, Kyle ,  MANDAVILLI, Bhaskar ,  YING, Lai-Qiang ,  BRANCHAUD, Bruce

    Inventor： KANG, Hee Chol ,  GEE, Kyle ,  MANDAVILLI, Bhaskar ,  YING, Lai-Qiang ,  BRANCHAUD, Bruce

    IPC: A61Q5/10 ,  A61K8/41

    CPC classification number: G01N21/6486 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

    Abstract: ABSTRACT Described herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

    Abstract translation: 摘要本文描述了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原染料，包括基于Cy的氢化花青染料和Cy-基氘异氰染料，其染料是用于检测ROS的探针和测量体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

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