公开(公告)号：WO2007147110A3

公开(公告)日：2008-11-27

申请号：PCT/US2007071327

申请日：2007-06-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  LYLE JOHN ,  PELUSO PAUL ,  SHEN GENE

Inventor： LYLE JOHN ,  PELUSO PAUL ,  SHEN GENE

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q2561/113 ,  C12Q2525/186 ,  C12Q2523/313

Abstract: Controlled initiation of primer extension in determination of nucleic acid sequence information by incorporation of nucleotides or nucleotide analogs. Preferred aspects include photo-initiated extension through the use of photo-cleavable blocking groups on termini of primer sequences followed by non-terminating primer extension using nucleotides or nucleotide analogs that are not extension terminators.

Abstract translation: 通过掺入核苷酸或核苷酸类似物来测定核酸序列信息中引物延伸的控制引发。 优选的方面包括通过在引物序列的末端使用可光切割的封闭基团，然后使用不是延伸终止子的核苷酸或核苷酸类似物进行非终止引物延伸的光引发延伸。

公开(公告)号：EP2029780A4

公开(公告)日：2010-03-31

申请号：EP07798630

申请日：2007-06-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： LYLE JOHN ,  PELUSO PAUL ,  SHEN GENE

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q2561/113 ,  C12Q2525/186 ,  C12Q2523/313

公开(公告)号：EP3030683A4

公开(公告)日：2017-05-03

申请号：EP14834734

申请日：2014-08-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

IPC: C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

CPC classification number: C07H19/10 ,  C07F9/65586 ,  C07F9/65616

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract translation: 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。

公开(公告)号：EP2850086A4

公开(公告)日：2016-03-09

申请号：EP13791524

申请日：2013-05-20

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： YUE STEPHEN ,  SHEN GENE ,  SUN WEI-CHUAN

IPC: C07D495/04 ,  C07D487/04 ,  C09B23/00 ,  C09B23/01 ,  C09B23/06 ,  C09B23/08 ,  C09B57/00 ,  G01N21/64

CPC classification number: G01N21/6486 ,  C07D487/04 ,  C07D495/04 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

Abstract: The present invention provides heteroaryl functionalized cyanine dyes including a reactive functional moiety, or which are conjugated to a carrier molecule.

公开(公告)号：EP2252625A4

公开(公告)日：2014-07-02

申请号：EP09707425

申请日：2009-02-06

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： OTTO GEOFFREY ,  SHEN GENE ,  KONG XIANGXU ,  EMIG ROBIN

IPC: C07H21/00 ,  C12Q1/48

CPC classification number: C12Q1/48 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/686

Abstract: The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates.

Abstract translation: 本发明提供用于进行照明反应，特别是测序反应的方法和组合物，同时减轻和/或防止可能由长时间照射引起的对反应物的光损伤。 特别地，本发明提供了将光保护剂掺入包含报道分子和核苷多磷酸的缀合物中的方法和组合物。