公开(公告)号：WO2012027623A3

公开(公告)日：2012-06-21

申请号：PCT/US2011049247

申请日：2011-08-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  YUE STEPHEN ,  SHEN GENE ,  SUN DAVID

Inventor： YUE STEPHEN ,  SHEN GENE ,  SUN DAVID

IPC: C07D403/06 ,  C07D209/10 ,  C07D209/18 ,  C07D209/30 ,  C07D401/14 ,  C09B23/00

CPC classification number: C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/0815 ,  C07K5/1019 ,  C09B23/0025 ,  C09B23/0033 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/164 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/582 ,  G01N2333/9126

Abstract: The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

Abstract translation: 本发明提供了一类新颖的花青染料，它们用磺酸基官能化，连接部分有助于它们与其它物质和取代基结合，这些物质和取代基增加了水溶性，并优化了染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和试剂盒，包括染料及其缀合物。

公开(公告)号：WO2012021733A3

公开(公告)日：2012-08-02

申请号：PCT/US2011047468

申请日：2011-08-11

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

Inventor： YUE STEPHEN ,  WEBER ROBERT ,  KONG XIANGXU ,  FEDOROV ANDREI ,  LYLE JOHN

IPC: G01N33/52 ,  C12Q1/25 ,  C12Q1/68

CPC classification number: C12Q1/6869 ,  C12N13/00 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/54373 ,  G01N2333/91245 ,  G01N2333/916

Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.

Abstract translation: 用于通过向反应混合物中加入一种或多种光保护性化合物并允许反应进行一段时间，以减少和/或防止照明分析反应中的一种或多种反应物的光诱导损伤的组合物，装置，系统和方法 小于光致伤害阈值期。

公开(公告)号：WO2012027625A3

公开(公告)日：2012-06-28

申请号：PCT/US2011049249

申请日：2011-08-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  SHEN GENE ,  SEBO LUBOMIR ,  YUE STEPHEN ,  SUN DAVID ,  BROGLEY LOUIS ,  FEDEROV ANDREI

Inventor： SHEN GENE ,  SEBO LUBOMIR ,  YUE STEPHEN ,  SUN DAVID ,  BROGLEY LOUIS ,  FEDEROV ANDREI

IPC: C12N11/00 ,  C07D251/12 ,  C07D403/04 ,  C12Q1/25 ,  C12Q1/68 ,  G01N33/52

CPC classification number: C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/0815 ,  C07K5/1019 ,  C09B23/0025 ,  C09B23/0033 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/164 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/582 ,  G01N2333/9126

Abstract: The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.

Abstract translation: 本发明提供了一类新型的基于脚手架的标记聚合酶酶底物。 聚合酶底物具有连接荧光染料部分和核苷磷酸部分的多价核心或支架。 聚合酶底物具有多个荧光染料部分和/或多个核苷磷酸部分。 优选的多价核心包含三官能六元芳族部分。 本发明还提供了具有基于脚手架的标记的聚合酶底物的测序方法和试剂盒。

公开(公告)号：WO2012027618A3

公开(公告)日：2012-05-18

申请号：PCT/US2011049242

申请日：2011-08-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  SHEN GENE ,  YUE STEPHEN

Inventor： SHEN GENE ,  YUE STEPHEN

IPC: C07D403/06 ,  C07D209/10 ,  C07D209/18 ,  C07D209/30 ,  C09B23/00

CPC classification number: C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/0815 ,  C07K5/1019 ,  C09B23/0025 ,  C09B23/0033 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/164 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/582 ,  G01N2333/9126

Abstract: The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

Abstract translation: 本发明提供了一类新颖的花青染料，其用接头部分官能化，所述接头部分便于它们与增加水溶性的其他物质和取代基缀合，并优化染料的光学性质。 还提供了染料的缀合物，使用染料及其缀合物的方法和包括染料及其缀合物的试剂盒。

公开(公告)号：WO2012027625A2

公开(公告)日：2012-03-01

申请号：PCT/US2011049249

申请日：2011-08-25

Applicant: PACIFIC BIOSCIENCES CALIFORNIA ,  SHEN GENE ,  SEBO LUBOMIR ,  YUE STEPHEN ,  SUN DAVID ,  BROGLEY LOUIS ,  FEDEROV ANDREI

Inventor： SHEN GENE ,  SEBO LUBOMIR ,  YUE STEPHEN ,  SUN DAVID ,  BROGLEY LOUIS ,  FEDEROV ANDREI

IPC: C12N11/00 ,  C07D251/12 ,  C07D403/04 ,  C12Q1/25 ,  C12Q1/68 ,  G01N33/52

CPC classification number: C12Q1/6869 ,  C07D209/14 ,  C07D401/14 ,  C07D405/14 ,  C07H19/10 ,  C07K5/0815 ,  C07K5/1019 ,  C09B23/0025 ,  C09B23/0033 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/164 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N33/52 ,  G01N33/582 ,  G01N2333/9126

Abstract: The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.

公开(公告)号：EP3030683A4

公开(公告)日：2017-05-03

申请号：EP14834734

申请日：2014-08-05

Applicant: PACIFIC BIOSCIENCES CALIFORNIA INC

Inventor： SEBO LUBOMIR ,  HANES JEREMIAH ,  SHEN GENE ,  BROGLEY LOUIS ,  YUE STEPHEN ,  ZHENG FRANK ,  LAPIN YURI ,  LYLE JOHN ,  OSUNA HONEY ,  FEDOROV ANDREI

IPC: C12Q1/68 ,  C07F9/6558 ,  C07F9/6561 ,  C07H19/10 ,  G01N21/64 ,  G01N23/223

CPC classification number: C07H19/10 ,  C07F9/65586 ,  C07F9/65616

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract translation: 提供受保护的荧光试剂化合物及其合成方法。 这些化合物可用于各种基于荧光的分析方法，包括高密度分析大量高度多重光学反应，如单分子实时核酸测序反应。 这些化合物含有荧光染料元素，可以在理想的波长下以高灵敏度检测化合物，允许化合物特异性地被目标生物分子识别的结合元件和保护性屏蔽元​​件，其减少荧光染料元素之间的不希望的接触 和结合的目标生物分子，并因此减少了荧光染料元件对结合的目标生物分子的光损伤。

公开(公告)号：EP2850086A4

公开(公告)日：2016-03-09

申请号：EP13791524

申请日：2013-05-20

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： YUE STEPHEN ,  SHEN GENE ,  SUN WEI-CHUAN

IPC: C07D495/04 ,  C07D487/04 ,  C09B23/00 ,  C09B23/01 ,  C09B23/06 ,  C09B23/08 ,  C09B57/00 ,  G01N21/64

CPC classification number: G01N21/6486 ,  C07D487/04 ,  C07D495/04 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

Abstract: The present invention provides heteroaryl functionalized cyanine dyes including a reactive functional moiety, or which are conjugated to a carrier molecule.