公开(公告)号：DK76191D0

公开(公告)日：1991-04-25

申请号：DK76191

申请日：1991-04-25

Applicant: UNIV LELAND STANFORD JUNIOR ,  SCLAVO SPA

Inventor： RELMAN DAVID A ,  DOMENIGHINI MARIO ,  RAPPUOLI RINO ,  FALKOW STANLEY

IPC: C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61K39/395 ,  A61P37/04 ,  C07K14/235 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19 ,  C12N15/62 ,  C07K13/00 ,  C12N15/31 ,  C12N15/70

公开(公告)号：FI911973A0

公开(公告)日：1991-04-23

申请号：FI911973

申请日：1991-04-23

Applicant: UNIV LELAND STANFORD JUNIOR ,  SCLAVO SPA

Inventor： RELMAN DAVID A ,  DOMENIGHINI MARIO ,  RAPPUOLI RINO ,  FALKOW STANLEY

IPC: C12N15/09 ,  A61K31/70 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61K39/395 ,  A61P37/04 ,  C07K14/235 ,  C12N1/21 ,  C12N5/10 ,  C12N9/12 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19 ,  C07K

公开(公告)号：IT1223579B

公开(公告)日：1990-09-19

申请号：IT2315087

申请日：1987-12-22

Applicant: ENIRICERCHE SPA ,  SCLAVO SPA

Inventor： PEDRONI PAOLA ,  RIBOLI BARBARA ,  DE FERRA FRANCESCA ,  GRANDI GUIDO ,  TOMA SALVATORE ,  ARICO' BEATRICE ,  RAPPUOLI RINO

IPC: C12N1/20 ,  A61K38/00 ,  A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  A61P37/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/19 ,  C12N1/21 ,  C12N15/30 ,  C12P21/02 ,  C12R1/01 ,  C12R1/19 ,  C12N

Abstract: The cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis are described. The aminoacid sequence of the mature subunit, deduced from its necleotide sequence, is similar but not identical to that of the known pilins 2, 3 and 6. Polypeptides having the aminoacid sequence of the mature pilinic subunit or of regions thereof are particularly useful for the development of synthetic acellular vaccines against pertussis.

公开(公告)号：IT1223529B

公开(公告)日：1990-09-19

申请号：IT2308587

申请日：1987-12-18

Applicant: SCLAVO SPA

Inventor： BARTOLONI ANTONELLA ,  PIZZA MARIAGRAZIA ,  RAPPUOLI RINO

IPC: G01N33/569 ,  A61K39/00 ,  A61K39/12 ,  C07K14/235 ,  C12N1/19 ,  C12N1/21 ,  C12P21/02 ,  C12R1/07 ,  C12R1/19 ,  C12R1/645 ,  A61K

公开(公告)号：IT1223334B

公开(公告)日：1990-09-19

申请号：IT2248187

申请日：1987-11-02

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  BARTOLONI ANTONELLA ,  RAPPUOLI RINO

IPC: A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  A61K

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.

公开(公告)号：IT9019286D0

公开(公告)日：1990-02-07

申请号：IT1928690

申请日：1990-02-07

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  COVACCI ANTONELLO ,  NENCIONI LUCIANO ,  RAPPUOLI RINO

IPC: C12K

公开(公告)号：IT1213313B

公开(公告)日：1989-12-20

申请号：IT2131486

申请日：1986-07-30

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12K

Abstract: A cloned and sequenced ECORI fragment of Bordetella pertussis chromosomal DNA with 4696 base pairs contains the genes which code for the 5 subunits of the pertussis toxin or further fragments, where the fragments contain at least one gene which codes for at least one subunit of the pertussis toxin, characterised by a given sequence. Also claimed is pertussis toxin in which the subunits S1,S2, S3, S4 are characterised by their aminoterminal sequences.

公开(公告)号：IT1191657B

公开(公告)日：1988-03-23

申请号：IT1920886

申请日：1986-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO

IPC: C12N20060101 ,  C12N

Abstract: A cloned and sequenced ECORI fragment of Bordetella pertussis chromosomal DNA with 4696 base pairs contains the genes which code for the 5 subunits of the pertussis toxin or further fragments, where the fragments contain at least one gene which codes for at least one subunit of the pertussis toxin, characterised by a given sequence. Also claimed is pertussis toxin in which the subunits S1,S2, S3, S4 are characterised by their aminoterminal sequences.

公开(公告)号：IT8722481D0

公开(公告)日：1987-11-02

申请号：IT2248187

申请日：1987-11-02

Applicant: SCLAVO SPA

Inventor： PIZZA MARIAGRAZIA ,  BARTOLONI ANTONELLA ,  RAPPUOLI RINO

IPC: A61K39/00 ,  A61K39/10 ,  A61P31/04 ,  C07H21/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

Abstract: Immunologically active polypeptides with no or reduced toxicity useful for the preparation of an antipertussis vaccine. Method for the preparation of said polypeptides which comprises, cultivating a microorganismtransformed with a hybrid plasmid including the gene/s which codes for at least one of said polypeptides in a suitable medium and recovering the desired polypeptide from the cells or from the culture medium.

公开(公告)号：IT8619208D0

公开(公告)日：1986-01-28

申请号：IT1920886

申请日：1986-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO

IPC: C12N20060101 ,  C12N