Preparation of sample for charged-particle microscopy

    公开(公告)号:US09772265B2

    公开(公告)日:2017-09-26

    申请号:US15052313

    申请日:2016-02-24

    Applicant: FEI Company

    Abstract: A system and method for preparing a sample for study in a charged-particle microscope is disclosed. A sample holder comprises substantially parallel opposing faces connected by apertures spanned by a perforated membrane. Blotting material is placed against the outer membrane surface, and liquid films may then be deposited onto the inner membrane surface within each aperture where each aperture can contain a unique sample. Liquids from each sample flow through the perforations in the membrane to be absorbed by the blotting material. After completion of deposition of liquid samples, the sample holder is raised off the blotting material, leaving aqueous samples within the perforations of the membrane. The sample holder may then be immersed in a vitrifying bath of liquid oxygen to form a cryo-sample for microscopic imaging and analysis.

    PREPARATION OF SAMPLE FOR CHARGED-PARTICLE MICROSCOPY
    197.
    发明申请
    PREPARATION OF SAMPLE FOR CHARGED-PARTICLE MICROSCOPY 有权
    填充颗粒显微镜样品的制备

    公开(公告)号:US20160245732A1

    公开(公告)日:2016-08-25

    申请号:US15052313

    申请日:2016-02-24

    Applicant: FEI Company

    Abstract: A system for preparing a sample for study in a charged-particle microscope by: Providing a substantially planar sample holder having opposed faces substantially parallel to one another, comprising at least one aperture that connects said faces and across which a membrane has been mounted, which membrane comprises at least one perforation; Spanning a film of aqueous liquid across said perforation, which liquid comprises at least one study specimen suspended therein; Prior to said spanning step, placing a blotting sheet of blotting material in intimate contact with a first surface of said membrane, at a side distal from said sample holder; Depositing said aqueous liquid through said aperture and onto a second surface of said membrane, opposite said first surface; and Subsequently removing said blotting sheet from said membrane.

    Abstract translation: 一种用于制备用于在带电粒子显微镜中研究的样品的系统,其通过以下方式来制备:提供具有基本上彼此平行的相对面的基本平坦的样品保持器,所述样品保持器包括连接所述面并且已经安装有膜的至少一个孔, 膜包括至少一个穿孔; 跨过所述穿孔跨越水性液体膜,该液体包括悬浮在其中的至少一个研究样本; 在所述跨越步骤之前,在远离所述样品保持器的一侧放置吸印材料的印迹片与所述膜的第一表面紧密接触; 将所述水性液体通过所述孔沉积到与所述第一表面相对的所述膜的第二表面上; 然后从所述膜中除去所述印迹片。

    Methods, apparatus and systems for production, collection, handling, and imaging of tissue sections
    199.
    发明授权
    Methods, apparatus and systems for production, collection, handling, and imaging of tissue sections 有权
    组织切片的生产,收集,处理和成像的方法,装置和系统

    公开(公告)号:US09304067B2

    公开(公告)日:2016-04-05

    申请号:US13734121

    申请日:2013-01-04

    Abstract: Methods, apparatus and systems for collecting thin tissue samples for imaging. Thin tissue sections may be cut from tissue samples using a microtome-quality knife. In one example, tissue samples are mounted to a substrate that is rotated such that thin tissue sections are acquired via lathing. Collection of thin tissue sections may be facilitated by a conveyor belt. Thin tissue sections may be mounted to a thin substrate (e.g., by adhering thin tissue sections to a thin substrate via a roller mechanism) that may be imaged, for example, by an electron beam (e.g., in an electron microscope). Thin tissue sections may be strengthened before cutting via a blockface thinfilm deposition technique and/or a blockface taping technique. An automated reel-to-reel imaging technique may be employed for collected/mounted tissue sections to facilitate random-access imaging of tissue sections and maintaining a comprehensive library including a large volume of samples.

    Abstract translation: 用于收集用于成像的薄组织样本的方法,装置和系统。 可以使用切片机质量刀从组织样品切割薄组织切片。 在一个实例中,将组织样品安装到旋转的基底上,使得通过车床获得薄的组织切片。 通过传送带可以促进薄组织切片的收集。 薄组织切片可以例如通过电子束(例如,在电子显微镜中)被安装到薄基底(例如,通过将薄组织切片通过辊机构粘附到薄基底上)。 在通过块状薄膜沉积技术和/或块状胶带技术切割之前可以加强薄组织切片。 可以采用自动卷线对卷成像技术来收集/安装的组织切片,以促进组织切片的随机取景成像并维持包括大量样品的综合库。

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