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公开(公告)号：KR1020100117048A
公开(公告)日：2010-11-02
申请号：KR1020100038084
申请日：2010-04-23
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for producing lipase using a buffer mixing system is provided to produce fixed lipase with high activity and high stability. CONSTITUTION: A lipase is fixed on a carrier by covalent bond in a buffer mixing system. The lipase is Candida rugosa lipase or Rhizopus oryzae lipase. Candida rugosa lipase or Rhizopus oryzae lipase are fixed at the same time at pH 6.0-7.0 and 0.2-0.3 M of ion strength for 20-30 hours.
Abstract translation: 目的：提供使用缓冲混合系统生产脂肪酶的方法，以产生高活性和高稳定性的固定脂肪酶。 构成：在缓冲混合系统中，通过共价键将脂肪酶固定在载体上。 脂肪酶是假丝酵母（Candida rugosa）脂肪酶或米曲霉（Rhizopus oryzae）脂肪酶。 在pH 6.0-7.0和0.2-0.3M的离子强度的同时固定假丝酵母（Crizida）或者米曲霉（Rhizopus oryzae）脂肪酶20-30小时。
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22.发明授权
公开(公告)号：KR100797152B1
公开(公告)日：2008-01-23
申请号：KR1020060116688
申请日：2006-11-24
Applicant: 고려대학교 산학협력단
IPC: C12N1/16
Abstract: A method for improving production yield of Saccharomyces cerevisiae JUL3 having beta-glucan is provided to mass produce Saccharomyces cerevisiae JUL3 inexpensively by using an inexpensive medium and optimizing process parameters, and increase the content of beta-glucan having high physiological activity in Saccharomyces cerevisiae JUL3. A method for improving production yield of Saccharomyces cerevisiae JUL3(KFCC 11359P) having abundant beta-glucan comprises the steps of: determining the optimum medium composition, wherein the optimum medium compositions are 5-7% molasses, 15-19% corn steep liquor, 0.3-0.7% KH2PO4, 0.05-0.15% MgSO4 and trace amount of minerals through response surface methodology as a statistical analysis method; comparing the cell production amount of Saccharomyces cerevisiae JUL3 in the optimum medium composition; determining the culture conditions, wherein the culture conditions are 200-400 rpm and 1-3 vvm in 2.5 liter fermenter, which are required for scale-up by using the optimum medium composition; and optimizing the substrate feed rate(10-20 ml/h) and concentration(30-75%) required for optimization of fed-batch culture under the optimum medium composition and culture conditions.
Abstract translation: 提供了具有β-葡聚糖的酿酒酵母JUL3的产量提高的方法，通过使用便宜的培养基和优化工艺参数廉价地大量生产酿酒酵母JUL3，并且增加了在酿酒酵母JUL3中具有高生理活性的β-葡聚糖的含量。 提供具有丰富β-葡聚糖的酿酒酵母JUL3（KFCC 11359P）的生产产量的方法包括以下步骤：确定最佳培养基组成，其中最佳培养基组合物为5-7％糖蜜，15-19％玉米浆， 作为统计分析方法，通过响应面方法，0.3-0.7％KH 2 PO 4，0.05-0.15％MgSO 4和微量矿物质; 将酿酒酵母JUL3的细胞产生量与最佳培养基组成进行比较; 确定培养条件，其中培养条件为200-400rpm，2.5升发酵罐中1-3vvm，其通过使用最佳培养基组合物进行放大所需; 并优化在最佳培养基组成和培养条件下优化补料分批培养所需的底物进料速率（10-20ml / h）和浓度（30-75％）。
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公开(公告)号：KR101864024B1
公开(公告)日：2018-06-01
申请号：KR1020160110051
申请日：2016-08-29
Applicant: 고려대학교 산학협력단
Abstract: 본발명은효소반응용반응시스템에관한것으로서, 본발명의실시예에따른효소반응용반응시스템은내부에수용된용매(1) 내의기질(2)과효소(3)가반응하여소정의생성물(4)을생성하는반응조(10), 반응조(10) 내부로기질(2) 또는효소(3)를공급하는공급라인(20), 및반응조(10)와연결되어, 반응조(10) 내부의효소(3) 및생성물(4)을포함하는혼합물(5)이유입되고, 혼합물(5) 중에포함된생성물(4)을여과하여, 생성물(4)이분리된혼합물(5)을반응조(10)로재공급하는순환부(30)를포함한다.
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公开(公告)号：KR101763064B1
公开(公告)日：2017-07-31
申请号：KR1020160033159
申请日：2016-03-21
Applicant: 고려대학교 산학협력단
Abstract: 본발명은커피찌꺼기로부터높은효율로오일과당을생산하는방법으로서, 커피찌꺼기에알칼리용액을첨가하는단계; 상기커피찌꺼기에무극성용매를투여하여액상과고체상을분리하는단계; 상기분리된액상에서무극성용매를회수하고용매와오일을분리하는단계; 및상기고체상에가수분해효소를투여하여당을생산하는단계를포함하는, 방법에관한것이다.
Abstract translation: 本发明提供一种用于制造油果糖从咖啡渣一个高效率，所述方法包括加入碱溶液到咖啡渣; 将非极性溶剂应用于咖啡渣以分离液相和固相; 回收分离液相中的非极性溶剂并分离溶剂和油; 并将水解酶施用于固体以产生糖。
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Patent Agency Ranking
公开(公告)号：KR101745079B1
公开(公告)日：2017-06-09
申请号：KR1020150053256
申请日：2015-04-15
Applicant: 고려대학교 산학협력단
Abstract: 본발명은 2,3-부탄다이올을생산하는균주가접종된배지에금속아세테이트또는암모늄아세테이트를첨가하는단계를포함하는 2,3-부탄다이올생산방법에관한것이다. 본발명의생산방법은금속아세테이트또는암모늄아세테이트를균주에첨가함에따라상기균주의생육조건에영향을미치지않으면서 2,3-부탄다이올의생산량과생산성을향상시키는것이특징이며, 이에따라지속적인 2,3-부탄다이올의생산이가능하다.
Abstract translation: 本发明涉及生产2,3-丁二醇的方法，该方法包括向接种有产生2,3-丁二醇的菌株的培养基中加入金属乙酸盐或乙酸铵的步骤。 本发明的制造方法的特征在于，向菌株中添加金属乙酸盐或乙酸铵可提高2,3-丁二醇的收率和生产率，而不影响菌株的生长条件， 可以生产3-丁二醇。
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公开(公告)号：KR1020160112331A
公开(公告)日：2016-09-28
申请号：KR1020150037930
申请日：2015-03-19
Applicant: 고려대학교 산학협력단
CPC classification number: C12N15/63 , C12N9/0006 , C12N15/80 , C12R1/84 , C12Y101/99018
Abstract: 본발명은피키아파스토리스를이용한백색부후균유래의셀로비오스디하이드로게나제대량생산방법으로, 보다상세하게는대량생산을위한백색부후균유래의셀로비오스디하이드로게나제유전자를포함하는발현벡터및 상기발현벡터로형질전환된숙주세포에관한것이다. 본발명의재조합발현벡터 pPICZαA/CDH 로형질전환된숙주세포는셀로비오스디하이드로게나제를발현하며, 단백질의분리정제를용이하게하기위해 His-tag이결합되어있어비교적간단한친화성크로마토그래피 (affinity chromatography column)을이용해분리정제할 수있는것이특징이다.
Abstract translation: 本发明涉及使用巴斯德毕赤酵母的白腐真菌来源的纤维二糖脱氢酶的批量生产方法。 更具体地，本发明涉及包含编码用于批量生产的白腐真菌来源的纤维二糖脱氢酶的基因的表达载体，以及由表达载体转化的宿主细胞。 通过重组表达载体pPICZA / CDH转化的宿主细胞表达纤维二糖脱氢酶，并且包括与其结合的组氨酸（His）标签，以使蛋白质分离/纯化容易。 因此，分离/纯化可以使用相对简单的亲和色谱柱进行。
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公开(公告)号：KR101521195B1
公开(公告)日：2015-05-20
申请号：KR1020130059069
申请日：2013-05-24
Applicant: 고려대학교 산학협력단
Abstract: 본발명은신규한세팔로스포린 C의제조방법에관한것으로, 더욱구체적으로미생물을이용하여세팔로스포린 C를제조하는방법에있어서, 탄소원으로자일로스를포함하는배양배지에서아크레모니움크리소지늄 ()을배양하는것을특징으로하는세팔로스포린 C의제조방법에관한것이다. 본발명에따르면, 바이오매스의효과적인활용이가능하게되고, 자일로스를이용한세팔로스포린 C의생산을통해공정단가절감을실현할수 있는장점이있다.
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公开(公告)号：KR1020140055566A
公开(公告)日：2014-05-09
申请号：KR1020120122616
申请日：2012-10-31
Applicant: 고려대학교 산학협력단
CPC classification number: B82B1/008 , B01J13/025 , B82B3/0095 , B82Y40/00 , C12N9/00
Abstract: The present invention comprises: (a) a core unit formed by a combination of a magnetic substance and boron; (b) a silica shell unit embracing the core; (c) a surface part of an ultimate outskirt shell where the surface of the ultimate outskirt shell is reformed to a substituent selected from the groups of -NH2, -COOH, -NHS and -Biotin; and a magnetic core/shell nanoparticle consisting of an enzyme or a biomaterial fixated onto the surface part of the shell, and encapsulated by chitosan. According to the present invention, the encapsulated magnetic core/shell nanoparticle maintains the vitality of the enzyme or the biomaterial at a high standard even when re-used a number of times, and the structure of the enzyme or the biomaterial is stably protected.
Abstract translation: 本发明包括：（a）由磁性物质和硼的组合形成的核心单元; （b）包围该芯的二氧化硅壳单元; （c）终极外壳的表面部分，其中最终外裙壳的表面被重整成选自-NH 2，-COOH，-NHS和生物素的取代基; 以及由酶或生物材料组成的磁性核/壳纳米颗粒固定在壳的表面部分上并被壳聚糖包封。 根据本发明，包封的磁性核/壳纳米颗粒即使重复使用多次也能保持酶或生物材料的活力，甚至可以稳定地保护酶或生物材料的结构。
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公开(公告)号：KR1020130101689A
公开(公告)日：2013-09-16
申请号：KR1020120022609
申请日：2012-03-06
Applicant: 고려대학교 산학협력단
CPC classification number: B01J19/0093 , C12M21/18 , C12P19/12
Abstract: PURPOSE: A microreactor for synthesizing lactulose and a method for preparing lactulose are provided to cheaply produce lactulose which is one of prebiotics by a reaction with a substrate containing fructose and whey using beta-galactosidase immobilized on the microreactor and to enable biological serial production. CONSTITUTION: A microreactor (11) for synthesizing lactulose contains beta-galactosidase which reacts to a substrate containing whey and fructose and is immobilized by multi-wall carbon nanotubes as a linking group. A method for producing lactulose comprises the steps of: preparing a reaction solution containing beta-galactosidase, a buffer solution, and whey; injecting the reaction solution and a solution containing the multi-wall carbon nanotubes into the microreactor to immobilize beta-galactosidase onto the microreactor; and injecting the substrate into the microreactor.
Abstract translation: 目的：提供用于合成乳果糖的微反应器和制备乳果糖的方法，通过与固定在微反应器上的β-半乳糖苷酶固定在微反应器上的含有果糖和乳清的底物进行反应，廉价地生产作为益生元之一的乳果糖，并实现生物连续生产。 构成：用于合成乳果糖的微反应器（11）含有与含有乳清和果糖的底物反应的β-半乳糖苷酶，并通过作为连接基团的多壁碳纳米管固定。 制备乳果糖的方法包括以下步骤：制备含有β-半乳糖苷酶，缓冲溶液和乳清的反应溶液; 将反应溶液和含有多壁碳纳米管的溶液注入微反应器以将β-半乳糖苷酶固定在微反应器上; 并将基板注入微反应器。
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