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公开(公告)号：KR1020040045267A
公开(公告)日：2004-06-01
申请号：KR1020030018573
申请日：2003-03-25
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12N9/14
CPC classification number: C12N9/16 , A23K20/189 , C12N15/70
Abstract: PURPOSE: Phytase derived from Citrobacter braakii is provided, which phytase can be used in domestic animal feed because it has improved enzyme activity, cannot be decomposed in the stomach and intestines because it has tolerance to hydrolase. CONSTITUTION: The phytase derived from Citrobacter braakii has the following properties: (a) 47 kDa of molecular weight on SDS-PAGE, (b) pH 3.5 to 4.5 of optimal pH, (c) 45 to 55 deg. C of optimal temperature, (d) substrate specificity wherein the substrates are phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) 3.5 to 0.5 mM of Michaelis constant when the substrate is phytate, and (f) high tolerance to a protein decomposing enzyme selected from pepsin, trypsin, papain, elastase and pancreatin, wherein the phytase comprises the amino acid sequence set forth in SEQ ID NO: 2 in the N-terminal. A gene encoding the phytase derived from Citrobacter braakii has the nucleotide sequence set forth in SEQ ID NO: 6. A phytase producing Citrobacter braakii YH-15(KCCM 10427) is provided.
Abstract translation: 目的：提供从柠檬酸杆菌衍生的植酸酶，其中植酸酶可用于家畜饲料，因为它具有改善的酶活性，不能在胃和肠中分解，因为它具有对水解酶的耐受性。 构成：来自柠檬酸杆菌的植酸酶具有以下性质：（a）SDS-PAGE上分子量为47kDa，（b）最佳pH为3.5至4.5，（c）为45至55℃。 （d）底物特异性，其中底物是植酸盐，对硝基苯基磷酸盐，焦磷酸四钠，ATP或ADP，（e）当底物植酸时，3.5至0.5mM的米氏常数，和（f）高耐受性 涉及选自胃蛋白酶，胰蛋白酶，木瓜蛋白酶，弹性蛋白酶和胰酶的蛋白质分解酶，其中所述植酸酶包含N-末端中SEQ ID NO：2所示的氨基酸序列。 编码来自枸橼酸杆菌的植酸酶的基因具有SEQ ID NO：6所示的核苷酸序列。提供了产生柠檬酸杆菌YH-15（KCCM 10427）的植酸酶。
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公开(公告)号：KR1019990066717A
公开(公告)日：1999-08-16
申请号：KR1019980028442
申请日：1998-07-14
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12N15/18 , A01K67/027 , C12N5/10 , C12N15/63
Abstract: 본 발명은 범가자미 (
Verasper variegatus , spotted flounder) 성장호르몬 및 그의 유전자에 관한 것이다. 구체적으로, 본 발명은 범가자미 성장호르몬 및 그의 아미노산 서열, 그의 cDNA 유전자 및 그의 염기 서열 그리고 그의 일부 또는 전체를 포함하는 플라스미드 벡터 및 대장균 발현 벡터에 관한 것으로서, 상기 유전자는 유전공학적 방법으로 재조합 성장호르몬을 대량 생산하여 어류에 경구 투여하거나 속성장이 가능한 형질전환 어류를 제조하는데 유용하게 사용될 수 있다.-
公开(公告)号：KR1020160017436A
公开(公告)日：2016-02-16
申请号：KR1020140100924
申请日：2014-08-06
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: A01K61/00
Abstract: 본발명은극동산뱀장어렙토세팔루스의변태유도방법에관한것으로, 더욱상세하게는극동산뱀장어의자어인렙토세팔루스를수조에수용하여사육하되, 사육수의수온과유속을변화시켜실뱀장어로변태를유도하는변태유도방법에관한것이다.
Abstract translation: 本发明涉及一种诱导日本鳗鱼的头a变态的方法。 更具体地说，诱导日本鳗鱼的头虱的变态的方法适应并提高作为水箱中的日本鳗鱼的幼虫的leptocephalus，并且改变繁殖水的温度和流速以诱发变态 leptocephalus进入一个elver。
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公开(公告)号：KR101402645B1
公开(公告)日：2014-06-03
申请号：KR1020130142003
申请日：2013-11-21
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: Y02A40/81 , A01K63/10 , A01K63/047 , B08B9/0821
Abstract: The present invention relates to a cleaning system of an aquaculture tank for early-stage larval eels which includes: a mixing system for a liquid feedstuff which measures and controls the discharge velocity and the flow rate of the aquaculture water; an aquaculture tank main body which comprises an open top, a floor part having a certain area, and an outer wall in the shape of a polygon; a guide rail formed on the upper parts of a right side and a left side of the outer wall of the aquaculture tank main body; a sub-motor which moves along the guide rail; a water supplying pipe which moves inside the aquaculture tank according to the movement of the sub-motor joined thereto; a discharging pipe arranged on a center side of the inside of the aquaculture tank main body. The liquid feedstuff inside the aquaculture tank is automatically mixed at a proper flow rate to increase the survival rate of the early-stage larval eels. An inner space of the aquaculture tank is cleaned by the currents of the aquaculture water supplied by the water supplying pipe formed to be able to reciprocate along the guide rail formed on the upper part of the outer wall of the aquaculture tank main body.
Abstract translation: 本发明涉及一种用于早期幼虫鳗鱼的水产养殖池的清洁系统，包括：用于测量和控制排水速度和水产养殖水流量的液体饲料混合系统; 一种水产养殖箱主体，包括开口顶部，具有一定面积的地板部分和多边形形状的外壁; 形成在水产养殖箱主体的外壁的右侧和左侧的上部的导轨; 沿着导轨移动的副电动机; 供水管，其根据与其接合的副电动机的运动在水产养殖箱内移动; 排水管布置在水产养殖箱主体的内部的中心侧。 水产养殖池内的液体饲料以适当的流速自动混合，以提高早期幼虫鳗鱼的存活率。 水产养殖槽的内部空间被形成为能够沿着形成在水产养殖箱主体的外壁的上部的导轨往复运动的供水管供给的养殖水的水流清洗。
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公开(公告)号：KR1020090048954A
公开(公告)日：2009-05-15
申请号：KR1020070115083
申请日：2007-11-12
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12Q1/6888 , C12Q2600/124
Abstract: 본 발명은 열성 치사 유전자형 넙치 감별용 유전자 마커에 관한 것으로, 구체적으로 넙치 변태시기에 치사를 유도하는 넙치 열성 치사 유전자, 열성 치사 유전자형을 갖는 개체 감별용 유전자 마커 및 상기 유전자 마커를 이용한 상기 열성 치사 유전자형을 갖는 개체의 감별방법에 관한 것이다. 본 발명의 유전자 마커를 이용하여 넙치의 변태시기에 치사를 유도하는 유전자형을 갖는 개체의 감별에 유용하게 사용하여 보다 정확하고 효율적인 분자육종을 가능하게 할 수 있다.
넙치, 열성 치사 유전자, 유전자 마커, 변태-
Patent Agency Ranking26.发明公开
公开(公告)号：KR1020060111144A
公开(公告)日：2006-10-26
申请号：KR1020050033562
申请日：2005-04-22
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12Q1/6844 , C12N15/11 , C12Q1/70 , C12Q2521/101 , C12Q2600/158
Abstract: A method for discrimination of individuals infected with lymphocystis disease virus(LCDV) or Paralichthys olivaceus individuals having resistance against lymphocystis disease virus is provided to produce of healthy individuals without virus infection, reduce lymphocystis disease occurrence rate by treating lymphocystis disease at an early stage, and selectively produce the excellent Paralichthys olivaceus individuals. A genetic marker for discrimination of fish individuals infected with lymphocystis disease virus has the nucleotide sequence of SEQ ID NO:10, wherein the genetic marker is amplified by a pair of primers having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, specific to NIF/NLI interacting factor gene; and the fish is Paralichthys olivaceus. The method for discrimination of fish individuals infected with lymphocystis disease virus comprises the steps of: (i) extracting the DNA sample of fish to be discriminated; (ii) amplifying the DNA sample with the primer pair of SEQ ID NO:1 and SEQ ID NO:2; and (iii) analyzing the amplification of the NIF/NLI interacting factor gene that is specific to the infection of lymphocystis disease virus.
Abstract translation: 提供一种鉴别感染淋巴囊病病毒（LCDV）或具有抗淋巴囊病病毒的Paralichthys olivaceus个体的个体的方法，以产生没有病毒感染的健康个体，通过早期治疗淋巴囊肿病减少淋巴囊肿病发生率，以及 选择性地产生优良的Paralichthys olivaceus个体。 用于鉴定感染淋巴囊病病毒的鱼类个体的遗传标记具有SEQ ID NO：10的核苷酸序列，其中遗传标记通过具有SEQ ID NO：1和SEQ ID NO：1的核苷酸序列的一对引物扩增。 2，特异性NIF / NLI相互作用因子基因; 鱼是Paralichthys olivaceus。 用于鉴别感染淋巴囊病病毒的鱼类个体的方法包括以下步骤：（i）提取待鉴别的鱼的DNA样品; （ii）用SEQ ID NO：1和SEQ ID NO：2的引物对扩增DNA样品; 和（iii）分析淋巴囊性疾病病毒感染特异的NIF / NLI相互作用因子基因的扩增。
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公开(公告)号：KR101423295B1
公开(公告)日：2014-07-25
申请号：KR1020140016382
申请日：2014-02-13
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: Y02A40/81 , A01K63/047 , A01K63/006
Abstract: The present invention relates to a donut-shaped water tank for an eel larva mass production having an integrated type water supply/drain pipe. The provided donut-shaped water tank is equipped with a feeding water rotation supply spray device which circulates at an appropriate flow rate so that larvae meet liquid feed in the tank which has a large diameter capable of accommodating the larvae in large quantities. Thus, the present invention reduces labor costs and prevents the mortality of larva by not only evenly mixing the liquid feed without forming a blind spot which is formed in an existing water tank when spraying feeding water or air but also automatically circulating the liquid feed in the water tank by controlling a feeding water spray device and the rotation speed of a sub motor for mixing the liquid feed. Also, the present invention can easily disassemble and assemble each component of a water tank main body, drain equipment, and the feeding water spray device which compose the water tank.
Abstract translation: 本发明涉及一种用于鳗鱼幼虫大量生产的环形水箱，其具有集成式供水/排水管。 所提供的甜甜圈型水箱配备有以适当的流量循环的给水旋转供给喷雾装置，使得幼虫在具有能够大量容纳幼虫的大直径的罐中满足液体进料。 因此，本发明通过不仅在喷射给水或空气的同时在形成于现有水箱中的盲点而均匀地混合液体进料而降低人工成本并且防止幼虫的死亡，而且还使液体进料自动循环 通过控制给水喷雾装置和用于混合进料的副电动机的转速来控制水箱。 而且，本发明可以容易地拆卸和组装水箱主体，排水设备和构成水箱的给水喷淋装置的每个部件。
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公开(公告)号：KR101402648B1
公开(公告)日：2014-06-03
申请号：KR1020130142013
申请日：2013-11-21
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: Y02A40/81 , A01K63/006 , A01K61/00
Abstract: The present invention provides an incubation system for fish larva which automatically mixes a liquid feed in an aquaculture tank at a proper flow rate to increase the survival rate of early-stage larval eels and to manage the early-stage larval eels in large amounts. The circulation rate and location of water is adjusted to exact figures which do not impose stress on the early-stage larval eels for an increased survival rate and mass production of the early-stage larval eels.
Abstract translation: 本发明提供了一种用于鱼类幼体的孵育系统，其以适当的流速自动混合水产养殖罐中的液体饲料，以提高早期幼虫鳗鱼的存活率并大量处理早期幼虫鳗鱼。 将水循环率和位置调整为对早期幼虫鳗鱼不施加压力的准确数字，以提高幼虫鳗鱼的存活率和大量产量。
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公开(公告)号：KR101184011B1
公开(公告)日：2012-09-27
申请号：KR1020100043855
申请日：2010-05-11
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C12N15/625 , C07K2319/02 , C07K2319/50 , C12N15/70 , C12P21/06
Abstract: The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.
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