公开(公告)号：KR1020080093488A

公开(公告)日：2008-10-22

申请号：KR1020070037227

申请日：2007-04-17

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김종만 ,  이희수 ,  김재명 ,  임숙경 ,  박신영 ,  장금찬 ,  임춘태 ,  정석찬

IPC: A61K36/233 ,  A61P37/00

Abstract: An extract of Bupleuri Radix is provided to increase various activities of main immunocytes of an animal such as granulocyte and macrophage and induce strong defence capability on attach inoculation of pathogenic bacteria. A composition comprising the same extract is provided to be used as an animal medicine such as an animal immuno-enhancing agent and a vaccine adjuvant and a feed additive for treating and preventing animal diseases efficiently. A method for purifying an extract of Bupleuri Radix comprises the steps of: (a) heat-treating the Bupleuri Radix with hot water at a temperature of 80-120 deg.C for 2-3 hours to obtain a crude extract; (b) concentrating the crude extract; and (c) filtering the concentrate to obtain a polysaccharide fraction by filtering paper and microorganism filtering. An extract of Bupleuri Radix purified by the method is a Bupleuri Radix polysaccharide, of which sugar is a glycoprotein including a protein. A composition for increasing immunity comprises the extract of Bupleuri Radix as an active ingredient and a pharmaceutically acceptable carrier or an excipient. An animal immunity enhancing agent, a vaccine adjuvant, or a feed additive for an animal comprises the composition for increasing immunity as an active ingredient.

Abstract translation: 提供柴胡提取物，以增加动物如粒细胞和巨噬细胞的主要免疫细胞的各种活性，并在附着接种病原菌时诱导强大的防御能力。 提供包含相同提取物的组合物用作动物药物，例如动物免疫增强剂和疫苗佐剂和用于有效治疗和预防动物疾病的饲料添加剂。 纯化柴胡提取物的方法包括以下步骤：（a）在80-120℃的温度下用热水热处理柴胡，2-3小时，得到粗提物; （b）浓缩粗提物; 和（c）过滤浓缩物以通过滤纸和微生物过滤获得多糖级分。 通过该方法纯化的柴胡提取物是一种柴胡甘草多糖，其中糖是包含蛋白质的糖蛋白。 用于增加免疫力的组合物包含作为活性成分的柴胡提取物和药学上可接受的载体或赋形剂。 动物免疫增强剂，疫苗佐剂或用于动物的饲料添加剂包括用于增加免疫力作为活性成分的组合物。

公开(公告)号：KR1020080084384A

公开(公告)日：2008-09-19

申请号：KR1020070026062

申请日：2007-03-16

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  김종만 ,  임숙경 ,  김재명 ,  박신영 ,  임춘태 ,  장금찬 ,  정석찬

IPC: A61K36/238 ,  A61P37/02

Abstract: An extract of Saposhnikovia root is provided to improve immune response by increasing activities of neutrophil, macrophage and lymphocyte, production of cytokines and non-specific protection against pathogenic bacteria infection in animals, so that it is useful as immunomodulator and immunoadjuvant. An extract of Saposhnikovia root for improving immune response in animals is prepared by heating Saposhnikovia root in water, and administered into an animal, especially domestic animals including cow, pig and chicken in the amount of 10-20 mg/kg per day through oral administration, intravenous injection, intramuscular injection or subcutaneous injection.

Abstract translation: 提供Saposhnikovia根的提取物，通过增加嗜中性粒细胞，巨噬细胞和淋巴细胞的活性，产生细胞因子和非特异性保护免受动物中的致病细菌感染来改善免疫应答，从而可用作免疫调节剂和免疫佐剂。 通过在水中加热山茱萸碱来制备Saposhnikovia根的提取物，并通过口服给药至每天10-20mg / kg的动物，特别是包括牛，猪和鸡的家畜，其量为10-20mg / kg ，静脉注射，肌内注射或皮下注射。

公开(公告)号：KR100270930B1

公开(公告)日：2000-11-01

申请号：KR1019980002745

申请日：1998-02-02

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 우승룡 ,  임숙경 ,  이희수 ,  김종만 ,  김종염

IPC: C07K16/12 ,  C12N7/01

Abstract: PURPOSE: A fusion cell line producing a monoclonal antibody specifically binding to E. coli O157 is provided to rapidly and exactly detect the infection of E. coli O157. CONSTITUTION: The microorganism NVRIO157(KCTC 0429BP) producing a monoclonal antibody specifically binding to E. coli O157 is produced by the steps of: immunizing a mouse with E. coli O157:H7 by injecting E. coli O157:H7 as an antigen into the mouse; separating immune cells that produce an antibody; and fusing the immune cells with myeloma cells to produce NVRIO157(KCTC 0429BP). The monoclonal antibody MNVRIO157 specifically binding to E. coli O157:H7 is produced from NVRIO157(KCTC 0429BP). The specificity of monoclonal antibody MNVRIO157 is analyzed by ELISA.

Abstract translation: 目的：提供产生与大肠杆菌O157特异性结合的单克隆抗体的融合细胞系，快速准确地检测大肠杆菌O157的感染。 构成：通过以下步骤产生产生与大肠杆菌O157特异性结合的单克隆抗体的微生物NVRIO157（KCTC 0429BP）：通过将大肠杆菌O157：H7作为抗原注射入到大肠杆菌O157：H7中免疫小鼠 老鼠; 分离产生抗体的免疫细胞; 并将免疫细胞与骨髓瘤细胞融合以产生NVRIO157（KCTC 0429BP）。 特异性结合大肠杆菌O157：H7的单克隆抗体MNVRIO157由NVRIO157（KCTC 0429BP）生产。 通过ELISA分析单克隆抗体MNVRIO157的特异性。