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公开(公告)号：KR1019970007027B1
公开(公告)日：1997-05-02
申请号：KR1019930030714
申请日：1993-12-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/55
Abstract: This invention relates to a gene encoding chitinase (pCHI1, KFCC-10807) isolated from Xanthomonas sp. obtained from soil. Since this enzyme exhibited strong chitinase activity and antifungal activity against Candida sp., this can be used to protect plant pathogens which contain chitin as major cell wall component. This gene can be incorporated into plant chromosome by transforming with Agrobacterium tumefaciens to generate transgenic plants which are resistant against fungal infection.
Abstract translation: 本发明涉及从黄单胞菌属（Xanthomonas sp。）分离的编码几丁质酶（pCHI1，KFCC-10807）的基因。 从土壤中获得。 由于该酶对假丝酵母菌表现出强烈的几丁质酶活性和抗真菌活性，因此可用于保护含有壳多糖作为主要细胞壁成分的植物病原体。 该基因可以通过用根癌土壤杆菌转化产生转基因植物而被掺入到植物染色体中，以产生耐真菌感染的转基因植物。
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公开(公告)号：KR1019960009742B1
公开(公告)日：1996-07-24
申请号：KR1019940004134
申请日：1994-03-03
Applicant: 대한민국(농촌진흥청장)
Abstract: The extraction method of edible oil (I) from plant Thalictrum Linne (II) consists of (1) crushing freeze-dried (II), and (2) extracting with chloroform and methanol. The edible seed oil is extracted by : freezing-drying Thalictrum Linne seed; pulverizing and utrasonicating to obtain the seed powder; extracting oil from the powder with chloroform and methanol; and purifying the extracted oil to obtain the final product. The seed oil has unsaturated fatty acid e.g. delta5-trans-linoleic acid.
Abstract translation: 植物枸杞（II）食用油（I）的提取方法为（1）粉碎冷冻干燥（II），（2）用氯仿和甲醇萃取。 可食用的种子油是通过以下方式提取的：冷冻干燥的Thalictrum Linne种子; 粉碎和松散以获得种子粉末; 用氯仿和甲醇从粉中提取油; 并纯化提取的油以获得最终产物。 种子油具有不饱和脂肪酸，例如 δ5-反式亚油酸。
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23.发明公开알파-1,3-푸코실트랜스퍼라아제 및/또는 베타-1,2-자일로실트렌스퍼라아제 유전자를 포함하는 벼 형질전환용 벡터 및 형질전환된 벼 有权用于包含基因编码的α-谷氨酸转移酶和/或β-1,2-酪氨酸转移酶的转基因载体和使用其的转基因大米
公开(公告)号：KR1020120089944A
公开(公告)日：2012-08-16
申请号：KR1020100138833
申请日：2010-12-30
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8218 , C12N9/1051 , C12N9/1077 , C12N15/113 , C12N15/8205 , C12N15/8251 , C12N2310/14 , C12Y204/01065 , C12Y204/02038
Abstract: PURPOSE: A vector for transforming Oryza sativa containing alpha-1,3-fucosyltransferase and/or beta-1,2-xylosyltransferase gene is provided to produce humanized glycoprotein. CONSTITUTION: A vector for transforming Oryza sativa contains a gene having a base of sequence numbers 1-3. The gene is inserted in forward and reverse directions. Bacteria are transformed with the vector. The bacteria are Agrobacterium. Oryza sativa transformed with the vector suppresses the expression of alpha-1,3-fucosyltransferase or beta-1-xylosyltransferase proteins. A method for producing humanized glycoprotein comprises: a step of introducing the vector to Oryza sativa cells and expressing glycoprotein the cells.
Abstract translation: 目的：提供用于转化含有α-1,3-岩藻糖基转移酶和/或β-1,2-木糖基转移酶基因的水稻的载体以产生人源化糖蛋白。 构成：用于转化水稻的载体含有具有序列号1-3的碱基的基因。 基因插入正反方向。 用载体转化细菌。 细菌是农杆菌。 用载体转化的水稻抑制α-1,3-岩藻糖基转移酶或β-1-木糖基转移酶蛋白的表达。 一种生产人源化糖蛋白的方法包括：将载体引入水稻细胞并将细胞表达糖蛋白的步骤。
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24.发明公开플라스미노겐 액티베이터 유전자를 함유하는 식물 형질전환용 벡터, 형질전환체 및 이를 이용한 플라스미노겐 액티베이터의 생산방법 有权含有PLASMINOGENA激活剂基因的转基因植物的载体，转基因植物以及使用其的等离子体激活剂的生产方法
公开(公告)号：KR1020100109824A
公开(公告)日：2010-10-11
申请号：KR1020090028306
申请日：2009-04-01
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/82 , C12N15/29 , A01H1/00 , C07K14/415
Abstract: PURPOSE: A vector for transforming a plant, containing plasminogen activator (t-PA-prolaime) is provided to express plasminogen activator protein to active form. CONSTITUTION: A vector for transforming plant comprises: a seed-specific glutelin promoter sequence, rice plant-derived glutelin protein signal sequence and plasminogen activator gene(t-PA), synthetic plasminogen activator gene(st-PA), plasminogen activator gene(t-PAER), His6-tagged plasminogen activator gene(t-PAHis6) and prolaime-attahced plasminogen activator gene(t-PA-prolamine) in order. The glutelin protein signal sequence has a base sequence of sequence number 2. The vector produces transgenic rice plant which expresses plasminogen activator by introducing to plant cells.
Abstract translation: 目的：提供用于转化含有纤溶酶原激活物（t-PA-prolaime）的植物的载体，以将纤溶酶原激活物蛋白表达为活性形式。 构成：用于转化植物的载体包括：种子特异性谷蛋白启动子序列，水稻植物衍生的谷蛋白信号序列和纤溶酶原激活物基因（t-PA），合成纤溶酶原激活物基因（st-PA），纤溶酶原激活物基因（t -PAER），His6标记的纤溶酶原激活物基因（t-PAHis6）和前体粘附的纤溶酶原激活物基因（t-PA-多巴胺）。 谷蛋白信号序列具有序列号2的碱基序列。载体产生通过引入植物细胞表达纤溶酶原激活物的转基因水稻植物。
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公开(公告)号：KR1020100015187A
公开(公告)日：2010-02-12
申请号：KR1020080076124
申请日：2008-08-04
Applicant: 대한민국(농촌진흥청장) , 성균관대학교산학협력단
Abstract: PURPOSE: A novel cholera vaccine containing an E2 antigen gene or transgenic plant expressing the gene is provided to produce an antibody to the E2 antigen and prevent cattle sickness. CONSTITUTION: An E2 antigen gene of swine cholera protein contains ER-tagged sequence or ER-retention sequence. The E2 antigen gene contains a sequence of sequence number 1. An E2 antigen protein contains amino acid sequence of sequence number 2. An expression vector for transforming a plant which is able to express E2 antigen contains the E2 antigen gene. The expression vector is E2/pB7WG2D(deposit number KACC 95087P). A plant which expresses the E2 antigen gene includes crop for livestock feed.
Abstract translation: 目的：提供含有E2抗原基因或表达该基因的转基因植物的新型霍乱疫苗，以产生E2抗原的抗体并防止牛病。 构成：猪霍乱蛋白的E2抗原基因含有ER标记的序列或ER-保留序列。 E2抗原基因含有序列号1的序列.E2抗原蛋白含有序列号2的氨基酸序列。用于转化能够表达E2抗原的植物的表达载体含有E2抗原基因。 表达载体为E2 / pB7WG2D（保藏号KACC 95087P）。 表达E2抗原基因的植物包括家畜饲料作物。
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Patent Agency Ranking
公开(公告)号：KR100929353B1
公开(公告)日：2009-12-02
申请号：KR1020080013524
申请日：2008-02-14
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 형질전환된 벼에 관한 것으로서, 더욱 상세하게는 수탁번호 KACC 95073P인 옥수수 RIP 유전자 발현 운반체(pARP7)로 형질전환된 벼에 관한 것이다.
벼 형질전환용 옥수수 RIP 유전자 발현 운반체(pARP7)를 이용하여 육성된 형질전환 벼와 동진벼를 교배하여 얻어진 우량 계통과 밀양 23호를 다시 교배하여 육성된 잡종강세가 우수한 벼의 형질전환 변이체 및 이를 이용한 벼의 잡종 종자 육종 및 생산할 수 있다.
본 발명에 따르면 인디카(indica)×자포니카(japonica) 간의 교잡을 포함하는 벼의 잡종강세를 이용한 육종시 출수기 지연, 불임 등의 난점을 효과적으로 극복할 수 있는 형질전환체를 이용한 잡종강세가 우수한 벼의 잡종종자 육종방법을 제공할 수 있다.
벼, 형질전환, 육종-
公开(公告)号：KR1020090088149A
公开(公告)日：2009-08-19
申请号：KR1020080013524
申请日：2008-02-14
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8261 , C12N15/8201 , C12N15/8216 , C12N15/8289
Abstract: A transgenic mutant of rice having excellent heterosis is provided to overcome problems such as infertility and delay of earning season using heterosis. A method for breeding transgenic mutant of hybrid rice having excellent heterosis comprises: a step of raising transgenic rice plant using corn RIP (ribosome-inactivating proteins) gene expression carrier (pARP7); and a step of cross-fertilizing Dongjin rice with transgenic rice plant to obtain superior line and cross-fertilizing Milyang 23 with the superior line again.
Abstract translation: 提供了具有优异杂种优势的水稻的转基因突变体，以克服诸如使用杂种优势的不孕不育和妊娠季节延迟等问题。 用于育种具有优异杂种优势的杂交稻的转基因突变体的方法包括：使用玉米RIP（核糖体失活蛋白）基因表达载体（pARP7）提高转基因水稻植物的步骤; 并与转基因水稻交配东京大米，通过再次优势上线，获得优良品种，交配Milyang 23。
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28.发明公开벼 유래 ＯｓＣＫ1 유전자, 이 유전자를 포함하는발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이형질전환체의 제조방법 有权来自ORYZA SATIVA的OSCK1基因，包含基因的表达载体，转化体转化的转化体和转化体的生产方法
公开(公告)号：KR1020060104772A
公开(公告)日：2006-10-09
申请号：KR1020050027204
申请日：2005-03-31
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 벼 유래 OsCK1 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 벼 내에서 과다발현시키면 벼의 벼흰잎마름병 및 벼도열병에 대한 저항성이 높아지는, 벼 유래 OsCK1을 코딩하는 서열번호 1로 기재되는 염기서열로 이루어지는 유전자, 이 유전자를 포함하는 발현벡터 pSBM-LS28, 이 발현벡터로 형질전환된 아그로박테리움 형질전환체(수탁번호:KACC 95034P) 및 벼 형질전환체 및 이 형질전환체의 제조방법에 관한 것이다.
벼, 콜린 카이나제, 형질전환체, 벼 도열병 저항성, 벼흰잎마름병 저항성-
公开(公告)号：KR1020040075385A
公开(公告)日：2004-08-30
申请号：KR1020030010830
申请日：2003-02-20
Applicant: 대한민국(농촌진흥청장)
IPC: A01H5/00
CPC classification number: C12N15/8274 , A01H4/005 , A01H4/008 , C12N5/0025 , C12N15/8205 , C12N15/8209 , C12N2500/34 , C12N2501/30
Abstract: PURPOSE: A method for producing transgenic perilla plants and herbicide-resistant perilla plants produced therefrom are provided, thereby easily inserting foreign useful genes into perilla plants, and reducing the production cost and environmental pollution. CONSTITUTION: The method for producing transgenic perilla plants comprises the steps of: (A) constructing an expression vector for transforming plants containing a foreign target gene and a selection maker; (B) introducing the expression vector into Agrobacterium to produce transformed Agrobacterium; (C) co-culturing the transformed Agrobacterium with a tissue section of perilla plants; (D) inducing shoots of the tissue section of perilla plants co-cultured in a selection-shoot inducing medium and subculturing the shoots of perilla plants in a root-inducing medium; and (F) transplanting the root-induced perilla plants in the field.
Abstract translation: 目的：提供一种生产转基因紫苏植物和由其生产的除草剂抗性紫苏植物的方法，从而容易地将外来的有用基因插入紫苏植物中，降低生产成本和环境污染。 构成：生产转基因紫苏植物的方法包括以下步骤：（A）构建用于转化含有外源靶基因的植物和选择标记物的表达载体; （B）将表达载体导入农杆菌以产生转化的土壤杆菌; （C）用紫苏植物的组织切片共转化转化的土壤杆菌; （D）诱导在选择性芽诱导培养基中共培养的紫苏植物的组织切片的芽，并在根诱导培养基中传代培养紫苏植物的芽; 和（F）在田间移植根诱导的紫苏植物。
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