국내 고구마 잎말림병 감염 DNA 바이러스의 양방향성프로모터
    21.
    发明公开
    국내 고구마 잎말림병 감염 DNA 바이러스의 양방향성프로모터 有权
    使用甜菜的双向促销蛋白

    公开(公告)号:KR1020090112492A

    公开(公告)日:2009-10-28

    申请号:KR1020080038415

    申请日:2008-04-24

    Abstract: PURPOSE: A bidirectional promoter of sweet potato leaf curl infection DNA virus is provided to produce vector product in molecular biology field and molecular breeding industry. CONSTITUTION: A bidirectional promoter of sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 1. A method for identifying the promoter activity comprises: a step of isolating a site with promoter activity and analyzing cis-acting regulation site; a step of recombining promoter site by using gateway cloning to a plant expression vector for promoter analysis; a step of transforming the recombined promoter site to Agrobacteria tumefaciens strain GV3101; and a step of screening transformant.

    Abstract translation: 目的:提供甘薯叶卷曲感染DNA双向启动子,在分子生物学领域和分子育种工业中产生载体产物。 构成:甘薯叶卷曲感染DNA病毒的双向启动子由序列号1的序列表示。鉴定启动子活性的方法包括:分离具有启动子活性的位点并分析顺式作用调节位点的步骤; 通过使用网关克隆到用于启动子分析的植物表达载体来重组启动子位点的步骤; 将重组的启动子位点转化到根癌土壤杆菌菌株GV3101的步骤; 并筛选转化体的步骤。

    순무황화모자이크바이러스를 검출하기 위한 등온증폭 반응용 프라이머 조성물, 및 이의 이용
    27.
    发明公开
    순무황화모자이크바이러스를 검출하기 위한 등온증폭 반응용 프라이머 조성물, 및 이의 이용 有权
    用于检测TURNIP黄色马赛克病毒的循环介导的等温放大反应的PRIMER组合物及其用途

    公开(公告)号:KR1020140086230A

    公开(公告)日:2014-07-08

    申请号:KR1020120156467

    申请日:2012-12-28

    CPC classification number: C12Q1/6844 C12Q2527/101 C12Q2563/107

    Abstract: The present invention relates to a set of four primers for isothermal amplification reaction, a composition including the same, and a method for detecting Turnip yellow mosaic virus using the composition. The detection method according to the present invention can effectively detect all six mutants of the Turnip yellow mosaic virus without a professional device in a short time using the isothermal amplification method. In addition, the Turnip yellow mosaic virus can be rapidly diagnose with a naked eye under natural light using highly concentrated SYBR Green I. Therefore, the present invention facilitates early detection of virus which harms farms cultivating Brassicaceae crops such as cabbages and the like, thereby making it possible to rapidly and efficiently construct a system diagnosing the Turnip yellow mosaic virus.

    Abstract translation: 本发明涉及一组用于等温扩增反应的四种引物,含有该引物的组合物,以及使用该组合物检测芜菁黄花叶病毒的方法。 根据本发明的检测方法可以使用等温扩增方法在短时间内有效地检测芜菁黄花叶病毒的所有六种突变体,而无需专业装置。 另外,利用高度浓缩的SYBR Green I,可以在自然光下用肉眼快速诊断芜菁黄花叶病毒。因此,本发明有助于早期检测危害养殖甘蓝科作物如白菜等的农场,从而 使得可以快速有效地构建诊断芜菁黄花叶病毒的系统。

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