Abstract:
The present invention relates to a method for high production of recombinant bifunctional acetyl xylan esterase with a carbohydrate binding module derived from phanerochaete chrysosporium. Acetyl xylan esterase produced from yeast which is transformed to an acetyl xylan esterase gene with a carbohydrate binding module(CBM) derived from phanerochaete chrysosporium not only has an activity of removing an acetyl group of a xylan backbone structure and decomposing xylan and lignin, but also improves an activity of decomposing biomass when used with xylanase at the same time, thereby being used as an industrial enzyme for feed additives, production of paper, a detergent, decomposition of lingo-cellulose-based biomass, and production of bio-energy.
Abstract:
본 발명은 밀기울 (wheat bran), 인산이수소칼륨 (KH 2 PO 4 ) 및 황산수소칼륨 (KHSO 4 )을 포함하는 것을 특징으로 하는 자일라나아제 (xylanase)를 생산하는 재조합 피키아 파스토리스 ( Pichia
pastoris ) 균주의 자일라나아제 (xylanase) 생산용 배지 조성물 및 자일라나아제를 생산하는 재조합 피키아 파스토리스 균주를 상기 자일라나아제 (xylanase) 생산용 배지 조성물이 함유된 발효조에 투입하여 배양하는 단계를 포함하는 자일라나아제 생산 방법을 제공한다.
Abstract:
PURPOSE: A recombinant expression vector for producing a large amount of endo-1,4-xylanase-A derived from Phanerochaete chrysosporium is provided to bleach pulp paper and to decompose hemicellulose. CONSTITUTION: A recombinant expression vector for producing a large amount of endo-1,4-beta xylanase-A is linked with a nucleic acid of sequence number 2 which encodes endo-1,4-beta xylanase-A without a nucleic acid sequence encoding AOXI promoter which is controlled by methanol, a nucleic acid sequence encoding an alpha-secretion factor derived from yeast, and a secretion signal sequence derived from Phanerochaete chrysosporium. The Phanerochaete chrysosporium is Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147). The yeast which produces endo-1,4-beta xylanase-A is prepared by transforming with the recombinant expression vector. The yeast is Pichia pastoris.
Abstract:
PURPOSE: A medium composition for culturing algae and a culturing method are provided to improve product amount of micro algae and to recue carbon dioxide. CONSTITUTION: A medium composition for culturing microalgae contains 0.4-1.0% of alginic acid as an active ingredient. The microalgae are Nannochloropsis sp. Nannochloropsis sp. is Nannochloropsis oculata. The medium is prepared with a composition containing alginic acid. A method for culturing microalgae comprises a step of culturing the microalgae using the culture medium at pH 7.0-pH 8.75.
Abstract:
PURPOSE: A method for producing a large amount of endo-1,4-beta xylanase A using a recombinant vector is provided to isolate and purify the enzyme by affinity chromatography. CONSTITUTION: A recombinant expression vector for producing a large amount of endo-1,4-beta xylanase A comprises: a nucleic acid sequence encoding an AOXI promoter regulated by methanol; and a nucleic acid sequence encoding endo-1,4-beta xylanase A containing secretion signal sequence derived from white rot fungi. The white rot fungi are Phaenerocheate chrysosporium BKM-F-1767(KCTC 6147). The endo-1,4-beta xylanase A containing white rot fungi-derived secretion signal sequences is a nucleic acid sequence of sequence number 1.
Abstract:
본 발명은 미생물 반응기용 수냉식 LED 조명기구에 관한 것으로써, 상부판의 하부 중앙에 하측으로 돌출형성되는 열전달부를 통해 상기 상부판에 형성되는 유로내를 순환하는 냉각수로 LED가 발산하는 열을 집중시켜 상기 LED가 발산하는 열과 냉각수를 보다 용이하게 열교환시킬 수 있음은 물론 이로 인해 상기 LED가 발산하는 열의 온도를 낮춰 상기 LED와 인쇄회로기판 등의 수명을 연장시킬 수 있게 되고, 나아가, 상기 상부판의 유로내를 순환하는 냉각수를 통해 고온의 반응기의 내부에 수용된 상기 LED의 온도를 낮출 수 있기 때문에 고온의 반응기의 내부에 수용된 상기 LED와 상기 LED를 보호하는 투명판이 상기 반응기의 고온의 열에 의해 파손될 우려를 보다 용이하게 방지할 수 있는 효과가 있다.