公开(公告)号：KR100892591B1

公开(公告)日：2009-04-08

申请号：KR1020070117886

申请日：2007-11-19

Applicant: 한국과학기술연구원

Inventor： 이창준 ,  노은주 ,  오수진 ,  박정환 ,  이재균

IPC: C07C229/58 ,  C07C229/52

CPC classification number: C07C229/58

Abstract: A chloride channel blocking agent containing anthranilic acid derivative is provided to show excellent validity and selectivity which result from excellent blocking activities of the anthranilic acid derivative. An anthranilic acid derivative is a compound having a structure represented by the formula I. In the formula I, R1 and R2 are independently selected from the group consisting of pentyl group, hexyl group, heptyl group, octyl group, decyl group, dodecyl group, tetradecyl group, hexadecyl group, cyclohexyl group, phenyl group, benzyl group, racemic-alphamethyl-benzyl group, chiral-alpha methyl-benzyl group, trifluoromethyl group, ethoxy group, butyloxy group, pentyloxy group, hexyloxy group, trifluoromethyloxy group and nitro group or two identical or different halogen atoms.

Abstract translation: 提供含有邻氨基苯甲酸衍生物的氯化物通道阻断剂，以显示由于邻氨基苯甲酸衍生物的优异的阻断活性而产生的优异的有效性和选择性。 邻氨基苯甲酸衍生物是具有由式I表示的结构的化合物。在式I中，R 1和R 2独立地选自戊基，己基，庚基，辛基，癸基，十二烷基， 十四烷基，十六烷基，环己基，苯基，苄基，外消旋 - 甲基 - 苄基，手性-α甲基 - 苄基，三氟甲基，乙氧基，丁氧基，戊氧基，己氧基，三氟甲氧基和硝基 或两个相同或不同的卤素原子。

公开(公告)号：KR1020090052521A

公开(公告)日：2009-05-26

申请号：KR1020070119060

申请日：2007-11-21

Applicant: 한국과학기술연구원

Inventor： 전혜성 ,  오수진 ,  유연규 ,  김윤경

IPC: G01N33/533 ,  G01N33/53 ,  G01N33/68 ,  C12N15/00

CPC classification number: G01N33/5035 ,  C07K2319/60 ,  C07K2319/735 ,  G01N33/582 ,  G01N2333/02 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/85 ,  C12N2730/10111 ,  G01N33/6845 ,  G01N2500/00

Abstract: The present invention relates to a method for screening an inhibitory agent of HBV proliferation by measuring the interaction (binding strength) between capsid protein and surface protein, necessary for the proliferation of HBV, by using cellular imaging, more precisely a method for measuring changes on cellular imaging caused by the interaction between a fusion protein containing PreS domain of HBV surface protein and PH (Pleckstrin homology) domain sequence and a fusion protein containing capsid protein and fluorescence protein (GFP) interacting with the said fusion protein. The method of the present invention detecting the interaction between proteins necessary for HBV proliferation at cellular level can be effectively used for the screening of a novel inhibitory agent of HBV proliferation at cellular level.