Abstract:
본 발명은 막걸리박 유래 당단백 분획 및 이의 제조방법에 관한 것으로, 좀 더 상세하게는 (a) 막걸리박을 탈지하는 단계; (b) 막걸리박을 pH가 7.0 내지 8.0인 완충용액에 혼합하는 단계; (c) 상기 혼합물을 원심분리하여 상등액을 수득하는 단계; (d) 상기 상등액을 투석 반투막(MWCO 6000-8000)으로 투석하는 단계; (e) 상기 투석된 상등액에 단백질을 침전시키는 염을 첨가하여 당단백질을 침전시키는 단계; (f) 상기 침전물을 pH가 7.0 내지 8.0인 완충용액에 용해하여 투석 반투막(MWCO 6000-8000)으로 투석하는 단계; 및 (g) 상기 투석된 침전 용해물에서 당단백 분획물을 수득하는 단계를 포함하는 방법에 의하여 제조되고, 분자량이 15 내지 110kDa인 것을 특징으로 하는 막걸리박 유래 당단백 분획물 및 이의 용도에 관한 것이다. 본 발명의 당단백 분획물은 대식세포의 활성을 면역활성 증진용 의약품인 크레스틴(PSK) 보다도 촉진하는 효능이 매우 뛰어나며 세포독성이 전혀 없어, 새로운 기능성 식품 및 면역기능 증강용 의약품 제조에 효과적이다.
Abstract:
The present invention relates to a pharmaceutical composition for preventing or treating obesity or metabolic diseases caused by obesity, containing a surfactant separated from a culture fluid of pseudomonas microorganism as an active ingredient. Also, the present invention relates to health functional food for preventing or alleviating obesity or metabolic diseases caused by obesity, containing surfactant separated from a culture fluid of pseudomonas microorganism as an active ingredient. According to the present invention, the surfactant can be used for an anti-obesity or weight control purpose by suppressing lipid digestion.
Abstract:
The present invention relates to a fraction of wheat bran-derived glycoprotein and a method of producing the same and, more particularly, to a fraction of wheat bran-derived glycoprotein, which is characterized by having a molecular weight of 16 to 70 kDa and prepared by a method comprising: (a) step of removing fat in wheat bran; (b) a step of mixing the wheat bran in a buffer solution having a pH level of 7.0 to 8.0; (c) a step of obtaining a supernatant by centrifuging the mixture; (d) a step of dialyzing the supernatant with a dialysis semipermeable membrane (MWCO 6000-8000); (e) a step of adding a salt precipitating protein in the dialyzed supernatant to precipitate glycoprotein; (f) a step of dissolving the precipitate in a buffer solution having a pH level of 7.0 to 8.0 with a dialysis semipermeable membrane (MWCO 6000-8000); and (g) a step of obtaining a fraction of glycoprotein from the dialyzed and precipitated lysate. The present invention also relates to a pharmaceutical food composition containing the fraction as an active ingredient for enhancing the immune function. The fraction of glycoprotein of the present invention is more effective in promoting histiocyte vitality than Krestin (PSK), which is a medicine for enhancing immune vitality, and has no cytotoxicity, thereby being effective in producing a new functional food and a medicine for enhancing the immune function.