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公开(公告)号：KR1020130046324A
公开(公告)日：2013-05-07
申请号：KR1020110111449
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/689 , C12Q1/6837 , C12R1/01
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Cronobacter sakazakii, and a DNA chip containing the same are provided to quickly and accurately detect Cronobacter sakazakii. CONSTITUTION: A probe for detecting bacteria causing food poisoning, Cronobacter sakazakii contains one or more among sequence lists 40-50. A DNA chip for detecting Cronobacter sakazakii contains the probe and quickly and accurately detects Cronobacter sakazakii. Cronobacter sakazakii is prepared from ATCC(American Type Culture Collection).
Abstract translation: 目的：提供用于检测引起食物中毒的细菌的探针，阪崎肠杆菌和含有这些细菌的DNA芯片，以快速准确地检测阪崎肠杆菌。 构成：检测细菌引起食物中毒的探针，克罗地球梭菌在序列表40-50中含有一个或多个。 用于检测克罗地球梭菌的DNA芯片含有探针，并快速准确地检测阪崎肠杆菌。 克罗伯杆菌阪崎肠杆菌由ATCC（美国典型培养物保藏中心）制备。
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公开(公告)号：KR1020130046321A
公开(公告)日：2013-05-07
申请号：KR1020110111446
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/689 , C12Q1/6837 , C12R1/445
Abstract: PURPOSE: A probe for detecting Staphylococcus aureus and a DNA chip containing the same are provided to quickly and accurately detect Staphylococcus aureus. CONSTITUTION: An oligonucleotide probe for a DNA chip for detecting bacteria causing foot poisoning, Staphylococcus aureus, contains one or more among sequence lists 25-40. The DNA chip contains the probe. The oligonucleotide probe quickly and accurately detects Staphylococcus aureus.
Abstract translation: 目的：提供金黄色葡萄球菌检测探针和含有金黄色葡萄球菌的DNA芯片，以快速，准确地检测金黄色葡萄球菌。 构成：用于检测引起足部中毒的细菌的DNA芯片的寡核苷酸探针，金黄色葡萄球菌，在序列表25-40中含有一个或多个。 DNA芯片包含探针。 寡核苷酸探针快速准确地检测金黄色葡萄球菌。
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Patent Agency Ranking
公开(公告)号：KR1020130046320A
公开(公告)日：2013-05-07
申请号：KR1020110111445
申请日：2011-10-27
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: Y02A50/451 , C12Q1/689 , C12Q1/6837 , C12R1/01
Abstract: PURPOSE: A probe for detecting bacteria causing food poisoning, Campylobacter jejuni, and a DNA chip containing the same are provided to quickly and accurately detect Campylobacter jejuni. CONSTITUTION: An oligonucleotide probe for a DNA chip for detecting Campylobacter jejuni contains one or more among sequence lists 26-50. The DNA chip contains the probe. The oligonucleotide probe quickly and accurately detects bacteria causing food poisoning, Campylobacter jejuni.
Abstract translation: 目的：提供用于检测引起食物中毒的细菌，空肠弯曲杆菌和含有其的DNA芯片的探针，以快速准确地检测空肠弯曲杆菌。 构成：用于检测空肠弯曲杆菌的DNA芯片的寡核苷酸探针在序列表26-50中含有一个或多个。 DNA芯片包含探针。 寡核苷酸探针快速准确地检测引起食物中毒的细菌，空肠弯曲杆菌。
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公开(公告)号：KR1020120129279A
公开(公告)日：2012-11-28
申请号：KR1020110047439
申请日：2011-05-19
Applicant: 한국외국어대학교 연구산학협력단
CPC classification number: C12Q1/04 , C12Q1/6844 , C12Q2535/139
Abstract: PURPOSE: A diagnosis method of virus using RAPD method is provided to simply and efficiently diagnose virus which do not have index gene which informs phylogenic location. CONSTITUTION: A diagnosis method of virus uses RAPD method(random amplified polymorphic DNA). A diagnosis method of virus comprises the following steps: amplifying DNA pieces of virus by applying RAPD PCR to virus which will be diagnosed; and comparing banding patterns of band by performing electrophoresis the amplified DNA pieces. A random primer manufacturing method used in RAPD PCR comprises the following steps: manufacturing primers comprised of 10 nucleotides by varying G+C content of primer base sequence and
Abstract translation: 目的：提供使用RAPD方法的病毒诊断方法，简单有效地诊断不具有通知系统发生位置的指标基因的病毒。 构成：病毒诊断方法采用RAPD法（随机扩增多态性DNA）。 病毒的诊断方法包括以下步骤：通过将RAPD PCR应用于待诊断的病毒来扩增DNA片段; 并通过对扩增的DNA片段进行电泳来比较条带的条带模式。 在RAPD PCR中使用的随机引物制备方法包括以下步骤：通过改变引物碱基序列的G + C含量和两端的<= G来制造由10个核苷酸组成的引物; 检查在制造的底漆上是否存在发夹或二聚体纹理发生; 并对在计算机中计算的效率最高的引物序列（等级= 100 +（<= G（Dimer）X 1.8 + <= G（发夹）X 1.4））进行实验分析。
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公开(公告)号：KR1020120007562A
公开(公告)日：2012-01-20
申请号：KR1020120001468
申请日：2012-01-05
Applicant: 한국외국어대학교 연구산학협력단 , 국민대학교산학협력단
Abstract: PURPOSE: An oligonucleotide for detecting gram positive bacteria causing food poisoning is provided to ensure quick and accuratel detection at once. CONSTITUTION: An oligonucleotide for detecting gram positive bacteria causing food poisoning is used for detect Bacillus cereus, Stapylococcus aureus, or Listeria monocytogenesis. The oligonucleotide for detecting Bacillus cereus contains a sequence of sequence number 1, 2, 3, 4, 5, or 6. The oligonucleotide for Stapylococcus aureus contains a sequence of sequence number 7, 8, 9, 10, 11, or 12. The oligonucleotide for detecting Listeria monocytogenesis contains a sequence of sequence number 13, 14, 15, or 16.
Abstract translation: 目的：提供用于检测引起食物中毒的革兰氏阳性菌的寡核苷酸，以确保快速和准确地检测。 构成：用于检测引起食物中毒的革兰氏阳性菌的寡核苷酸用于检测蜡状芽孢杆菌，金黄色葡萄球菌或单核细胞增生性李斯特菌。 用于检测蜡状芽孢杆菌的寡核苷酸含有序列号1,2,3,4,5或6的序列。金黄色金黄色葡萄球菌的寡核苷酸含有序列号7,8,9,10,11或12的序列。 用于检测李斯特菌单核细胞增生的寡核苷酸含有序列号为13,14,15或16的序列。
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公开(公告)号：KR101003846B1
公开(公告)日：2010-12-27
申请号：KR1020080051165
申请日：2008-05-30
Applicant: 한국외국어대학교 연구산학협력단 , 국민대학교산학협력단
Abstract: 본 발명은 리얼 타임 PCR 기법을 이용하여 과일 주스로부터 식중독 세균을 검출하는 방법에 관한 것이다. 구체적으로, 킬레이팅 레진과 겔 여과 칼럼을 이용하여 PCR 저해물질을 신속하게 제거함과 동시에 DNA 주형을 추출하고, 추출된 DNA 주형을 이용하여 리얼 타임 PCR을 수행함으로써 과일 주스 내에 존재하는 식중독 세균을 검출하는 것을 특징으로 한다. 이에 의하면, 기존의 농화배양과정, 전기영동과정을 거치지 않고서도 간편하고 신속하게 과일 주스 내의 식중독 세균을 검출할 수 있는 장점이 있다.
L.monocytogenes, chelating resin, gel filteration column, real-time PCR-
公开(公告)号：KR1020090078136A
公开(公告)日：2009-07-17
申请号：KR1020080003944
申请日：2008-01-14
Applicant: 서울대학교산학협력단 , 한국외국어대학교 연구산학협력단
CPC classification number: C12M47/02 , G01N1/4077
Abstract: A collection and detection method of microorganism in food through ultrafiltration is provided to analyze various kinds of microorganism in a small amount of sample and reduce time and expense for analysis of microorganism. A microorganism in food is collected and concentrated through ultrafiltration using hollow-fiber. The hollow-fiber is pretreated with bovine calf serum, NaPP, glycine-added FBS, beef extract, bovine serum albumin (BSA), or nutrient broth blocking solution. The microorganism is bacteriophage, virus, germ, prokaryote or spore. The virus is rotavirus or noroviurs. The microorganism is detected by using PCR (polymerase chain reaction) of collected and concentrated microorganism sample.
Abstract translation: 提供超滤食品中微生物的收集和检测方法，以分析少量样品中的各种微生物，并减少微生物分析的时间和费用。 通过使用中空纤维的超滤收集食物中的微生物并浓缩。 中空纤维用牛血清，NaPP，加入甘氨酸的FBS，牛肉提取物，牛血清白蛋白（BSA）或营养液阻断溶液预处理。 微生物是噬菌体，病毒，胚芽，原核生物或孢子。 病毒是轮状病毒或诺维诺病毒。 通过使用收集和浓缩的微生物样品的PCR（聚合酶链式反应）检测微生物。
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