公开(公告)号：JPS62221467A

公开(公告)日：1987-09-29

申请号：JP6300086

申请日：1986-03-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SORA TATSUO ,  MAEDA HIDEATSU ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

IPC: B05D1/18 ,  B05D7/24 ,  B41M5/26

Abstract: PURPOSE:To efficiently form a highly homogenous membrane layer, by treating the surface of a substrate with a coupling agent and forming a purple membrane-containing membrane layer due to the polymerization of acrylamide to the treated surface. CONSTITUTION:A purple membrane is the one isolated from Halobacterium halobium being one kind of halophil and contains bacteriorhodopsin being chromoprotein and is dissolved in an aqueous solution containing 5-15wt% of acrylamide to obtain a desired acrylamide solution. A substrate such as a glass substrate, an ITO electrode substrate or a metal plate is treated with a silane coupling agent and acrylamide is polymerized while the acrylamide solution containing the purple membrane is contacted with the surface of the surface treated substrate to form a homogenous membrane layer of a purple membrane-containing acrylamide gel.

公开(公告)号：JPS6297686A

公开(公告)日：1987-05-07

申请号：JP23740485

申请日：1985-10-25

Applicant: AGENCY IND SCIENCE TECHN ,  HITACHI LTD

Inventor： ASAI MICHIHIKO ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  TAKEMOTO KAZUNARI ,  KITO MAKOTO ,  KUROIWA MINORU ,  KOMATSU TAKASHI

IPC: C02F1/00 ,  C02F1/32 ,  C02F1/42 ,  C02F1/44 ,  C02F9/00 ,  C08J7/00

Abstract: PURPOSE:To reduce the elution of an org. substance, by forming a plasma polymerization layer to the inside surface of each of the piping between apparatuses and the piping up to an area using ultrapure water. CONSTITUTION:Primary pure water Wa supplied is subjected to sterilization treatment due to the irradiation of ultraviolet rays in an ultraviolet sterilizing apparatus 1 and introduced into an ion exchange apparatus 2 through piping 4A to remove the dissolved ion in the primary pure water Wa. The primary pure water Wa after the removal of the ion is introduced into an ultrafiltration apparatus 3 through piping 4B and fine particles contained in the primary pure water Wa are removed to obtain ultrapure water Wb which is, in turn, supplied to a use area through piping 4C. A plasma polymerization layer 6 comprising a monomer such as hydrocarbon, a nitrogen-containing compound and a fluorine- containing compound is formed to the piping main body of each of the pipings 4A-4C. By this method, ultrapure water is obtained.

公开(公告)号：JPS6246232A

公开(公告)日：1987-02-28

申请号：JP18523785

申请日：1985-08-23

Applicant: AGENCY IND SCIENCE TECHN

Inventor： HADO MASAKATSU ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

IPC: G01N13/00

Abstract: PURPOSE:To automatically and continuously measure the advance or retraction contact angle between specimens, by performing the limitation of the potential between a solid specimen and a liquid specimen and restricting the advance or retraction condition of the meniscus between both specimens. CONSTITUTION:The titled apparatus is constituted of an electric balance 1 having a mass measuring terminal part 2, a liquid surface minute speed moving mechanism 13, a measuring cell 5 having a cover 8 mounted to the upper part thereof and receiving a liquid specimen and a recorder 7 for continuously and automatically recording the output of the balance 1. Because a solid specimen 4 is electrically contacted with a hook 3, the solid specimen 4 is used as an acting electrode and an opposed electrode 9 and a standard electrode 11 are connected to a usual potentiostat 15 to regulate the potential of the solid specimen 4 according to a three-electrode method. When the plate 14 having the measuring cell 5 mounted thereto is electrically raised or fallen at a constant speed by the moving mechanism 13, a contact angle can be easily measured while the meniscus formed between the solid specimen 4 and the liquid specimen 6 is held to the state of an advance or retraction contact angle.

公开(公告)号：JPS62203367A

公开(公告)日：1987-09-08

申请号：JP4657786

申请日：1986-03-04

Applicant: AGENCY IND SCIENCE TECHN ,  MATSUSHITA ELECTRIC IND CO LTD

Inventor： SORA TATSUO ,  MAEDA HIDEATSU ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  OGAWA KAZUFUMI

IPC: H01L27/14 ,  H01L27/146 ,  H01L27/148 ,  H01L51/42 ,  H04N5/335 ,  H04N5/369 ,  H04N5/372

Abstract: PURPOSE:To permit the improvement of color reproducibility conforming a chrominance signal to the conditions that it is seen with human eyes by a method wherein a visual pigment film which generates photocurrent according to the intensity of a light is formed being pinched between a contact electrode and a transparent electrode. CONSTITUTION:A picture element part region 2 for sensing an optical image is formed at the central part of a substrate 1 and a driving part 3 of the picture element part 2 is provided on the periphery of this region. Then, a diffusion part 16 for charge storage and a contact electrode 12 to connect to a visual pigment film 11, which functions as a photosensor according to each picture element, are formed. Moreover, after the visual pigment film 11, which is one formed a visual pigment-containing cell membrane into a filmy form and functions as a photosensor, is laminated on the whole surface of the picture element part 2, a transparent electrode 10 is formed on the whole surface on the region 2 and a color filter 7 is installed on the front of the picture element part 2. Thereby, the color reproducibility can be improved being well-balanced a chrominance signal obtainable through the filter 7 to the conditions that it is seen with human eyes.

公开(公告)号：JPS6263823A

公开(公告)日：1987-03-20

申请号：JP20386885

申请日：1985-09-14

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SORA TATSUO ,  MAEDA HIDEATSU ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

IPC: G01J1/00 ,  C09K9/02 ,  G01J1/42 ,  H01L31/08 ,  H01L51/30 ,  H03F3/08

Abstract: PURPOSE:To make a wavelength characteristic of an optical sensor approximate to the visibility of a human being by using a visual matter for a photosensitive part. CONSTITUTION:A thin film 3 of a cell membrane containing bacteriorhodopsin which has been isolated from a halophile is electro-deposited onto a transparent electrode 2 which has been vacuum-evaporated on a glass substrate 1, and also the second electrode 4 is vacuum-evaporated on said film, by which a photosensitive part is formed. A source 6 and a gate 7 of a field effect transistor 5 are connected through a conductive circuit 11 to the transparent electrode 2 and the second electrode 4 of the photosensitive part, respectively, and a drain 8 is connected to the conductive circuit 12 to the source 6 through a DC power source 9 and a resistance 10, by which a detecting/amplifying part of a photoelectromotive force which has been generated in the photosensitive part is formed. A variation of a drain current caused by a light irradiation of the photosensitive part is recorded in a recorder 12, as a variation of an across voltage of the resistance 10.

公开(公告)号：JPS6239236A

公开(公告)日：1987-02-20

申请号：JP17946785

申请日：1985-08-16

Applicant: AGENCY IND SCIENCE TECHN ,  BRIDGESTONE CORP

Inventor： ASAI MICHIHIKO ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  AKIYAMA SETSUO ,  YOSHIKAWA MASAHITO ,  FUKUURA YUKIO ,  NAITO TOSHIO ,  HONDA TOSHIO

IPC: B32B25/04 ,  C08J5/12 ,  C08J7/04

公开(公告)号：JPS61191364A

公开(公告)日：1986-08-26

申请号：JP3160585

申请日：1985-02-21

Applicant: AGENCY IND SCIENCE TECHN ,  WATANABE YAKUHIN KOGYO KK

Inventor： KODAMA AKIRA ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  KOMAMIYA MASAHARU ,  OINUMA HIROSHI

IPC: A61L33/10 ,  A61L33/00

公开(公告)号：JPH02100681A

公开(公告)日：1990-04-12

申请号：JP25226588

申请日：1988-10-06

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IZUKURA MASAHIRO ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N15/09 ,  C07K1/16 ,  C07K1/22 ,  C07K14/00 ,  C07K14/575 ,  C07K14/655 ,  C07K14/675 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N15/12 ,  C12N15/62 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a recombinant plasmid pENDB 15 capable of producing beta-endorphin stably replicated in Escherichia coli and having strong analgesic activity and having DNA sequence of 4718 base pair. CONSTITUTION:The novel recombinant plasmid pENDB15 has size of 4718 base pair and provides trimethoprim resistance and ampicillin resistance to Escherichia coli which is a host. The above-mentioned plasmid is obtained by replacing sequence of 26 base pair containing sequence coding MEK between BamHI site of pMEK2 (patent application No.63-79680) and XhoI site with chemically synthesized DNA of 104 base pair containing sequence coding beta- endorphin. Escherichia coli transformed by the pENDB15 is deposited as FERM BP-2029 to Fermentation Research Institute.

公开(公告)号：JPH0279978A

公开(公告)日：1990-03-20

申请号：JP23124888

申请日：1988-09-14

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N15/09 ,  A61K38/22 ,  A61P23/00 ,  A61P25/04 ,  C07K1/16 ,  C07K1/22 ,  C07K14/00 ,  C07K14/575 ,  C07K14/655 ,  C07K14/675 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N15/16 ,  C12N15/62 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a recombinant plasmid pENDD2 being a recombinant plasmid having 4682 pair of bases and capable of producing delta-endorphin of morphine like peptide having high analgesic activity when Escherichia coli is transformed by the above-mentioned plasmid. CONSTITUTION:Gene coding delta-endorphin expressed by the formula is synthesized and the synthesized gene is integrated into a plasmid pMEK2 to prepare a fused gene (pENDCD2) of delta-endorphin gene and dihydrofolic acid reducing enzyme (DHFR) gene, by which Escherichia coli is then transformed. DHFR-delta- endorphin fused protein is produced by culturing the transformed Escherichia coli and then the fused protein is digested by a proteolytic enzyme to separate delta-endorphin.

公开(公告)号：JPH01252286A

公开(公告)日：1989-10-06

申请号：JP7967788

申请日：1988-03-31

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  SAKAI TSUKASA ,  TANAKA YOSHIO

IPC: C12N15/09 ,  C07K1/12 ,  C07K1/16 ,  C07K1/22 ,  C07K7/18 ,  C07K14/00 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a large amount of dihydrofolic acid reductase-bradykinin fused protein, by manifesting a fused gene in which a bradykinin gene is included in a plasmid vector pTP 70-1 with a colon bacillus. CONSTITUTION:A newly recombined plasmid pBK 1-11, which is replicated in stable with a colon bacillus, imparting resistance to trimethoprim and resistance to ampicillin to a colon bacillus as a host, having a size of 4645 basic pairs and having a bonded structure of chemically synthesized DNA of 37 basic pairs containing a conformation coding bradykinin at BamHI site of pTP 70-1, is produced. A colon bacillus introduced of pBK 1-11 (FERMBP-1817) produces a large amount of dihydrofolic acid reductase-bradykinin fused protein.