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    métodos para produção de metionina
    23.
    发明专利
    métodos para produção de metionina 未知

    公开(公告)号：BRPI0215958B1

    公开(公告)日：2016-11-08

    申请号：BR0215958

    申请日：2002-11-29

    Applicant: BASF AG ,  BASF SE ,  EVONIK DEGUSSA GMBH

    Inventor： KRÖGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR ,  SCHÖDER HARTWIG ,  POMPEJUS MARKUS ,  ZELDER OSKAR ,  HAEFNER STEFAN

    IPC: C07K14/34 ,  C12N1/21 ,  C12N15/31 ,  C12N15/77 ,  C12P13/12 ,  C12R1/13 ,  C12R1/15

    Abstract: "métodos de modulação da produção de um composto contendo enxofre por um microorganismo e de produção de metionina, cassete de expressão transgênico, vetor, e, célula hospedeira transgênica". moléculas de ácido nucleico, chamadas de moléculas de ácido nucleico mr, que codificam novas proteínas mr de corynebacterium glutamicum são descritas as quais estão envolvidas na biossíntese de um composto químico fino, por exemplo na biossintese de metionina. a invenção também proporciona moléculas de ácido nucleico de anti-senso, vetores e cassetes de expressão transgênicos contendo moléculas de ácido nucleico mr e células hospedeiras para dentro das quais os vetores ou cassetes de expressão têm sido introduzidos. a invenção ainda adicionalmente proporciona métodos de produção de metionina a partir de microorganismos, por exemplo c. glutamicum, que envolve a cultura de microorganismos recombinantes que superexpressam ou subexpressam pelo menos uma molécula mr da invenção sob condições tais que metionina é produzida. também são apresentados métodos de produção de um composto químico fino, por exemplo metionina, que envolve a cultura de microorganismos recombinantes possuindo genes mr selecionados deletados ou mutados sob condições tais que o composto químico fino, por exemplo metionina, é produzido.

    Pgro expression units
    24.
    发明专利
    Pgro expression units 未知

    公开(公告)号：IN439CH2008A

    公开(公告)日：2008-09-19

    申请号：IN439CH2008

    申请日：2008-02-21

    Applicant: BASF AG

    Inventor： KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

    IPC: C07K14/34 ,  C12N15/77 ,  C12P13/08

    Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

    27.
    发明专利
    未知

    公开(公告)号：BRPI0513227A

    公开(公告)日：2008-04-29

    申请号：BRPI0513227

    申请日：2005-07-16

    Applicant: BASF AG

    Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

    IPC: C12Q1/68 ,  C07K14/34

    Abstract: A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).

    Multiple promoters and the use thereof for gene expression
    30.
    发明专利
    Multiple promoters and the use thereof for gene expression 未知

    公开(公告)号：AU2005321577A1

    公开(公告)日：2006-07-06

    申请号：AU2005321577

    申请日：2005-12-21

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  SCHRODER HARTWIG ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA

    IPC: C12N15/77 ,  C07K14/34

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

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