公开(公告)号：CZ302494B6

公开(公告)日：2011-06-15

申请号：CZ20011382

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: Rešení se týká sekvence nukleových kyselin, která kóduje polypeptid s nitrilázovou aktivitou, konstruktu nukleových kyselin, zahrnujících tuto sekvenci nukleových kyselin a vektoru, zahrnujících tuto sekvenci nukleových kyselin nebo uvedené konstrukty nukleových kyselin. Dále se týká aminokyselinových sekvencí, které jsou kódovány uvedenou sekvencí nukleových kyselin a mikroorganizmu, zahrnujících uvedené sekvence nukleových kyselin, konstrukty nukleových kyselin nebo vektory, obsahující uvedené sekvence nukleových kyselin nebo konstrukty nukleových kyselin. Rešení se rovnež týká zpusobu prípravy chirálních karboxylových kyselin z racemických nitrilu, který využívá pripravenou nitrilázu.

公开(公告)号：NO20024169D0

公开(公告)日：2002-09-02

申请号：NO20024169

申请日：2002-09-02

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：NO20021931D0

公开(公告)日：2002-04-24

申请号：NO20021931

申请日：2002-04-24

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19 ,  C12N

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：DE10010149A1

公开(公告)日：2001-09-06

申请号：DE10010149

申请日：2000-03-03

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P41/00 ,  C12R1/19 ,  C12N9/00 ,  C07K14/00 ,  C07H21/00 ,  C12P21/02 ,  C12P7/42 ,  C12P17/00 ,  C12N15/11 ,  C12N1/20

Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).

公开(公告)号：NO20011912L

公开(公告)日：2001-04-18

申请号：NO20011912

申请日：2001-04-18

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12N15/09 ,  C12P41/00 ,  C12R1/05 ,  C12N5/10

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：CA2347521A1

公开(公告)日：2000-04-27

申请号：CA2347521

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOSCHKE MARION ,  HAUER BERNHARD ,  ENGELS DIRK ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N9/78 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05 ,  C12N15/63

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide with nitrilase activity, to nucleic acid constructs containing the nucleic acid sequences, and to vectors containing the nucleic acid sequences or the nucleic acid constructs. The invention also relates to amino acid sequences which are coded by the nucleic acid sequences, and to microorganisms containing the nucleic acid sequences, the nucleic acid constructs or vector s containing the nucleic acid sequences or the nucleic acid constructs. In addition, the invention relates to a method for producing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：CA2347521C

公开(公告)日：2010-02-23

申请号：CA2347521

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOSCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide with nitrilase activity, to nucleic acid constructs containing the nucleic acid sequences, and to vectors containing the nucleic acid sequences or the nucleic acid constructs. The invention also relates to amino acid sequences which are coded by the nucleic acid sequences, and to microorganisms containing the nucleic acid sequences, the nucleic acid constructs or vectors containing the nucleic acid sequences or the nucleic acid constructs. In addition, the invention relates to a method for producing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：NO327857B1

公开(公告)日：2009-10-05

申请号：NO20011912

申请日：2001-04-18

Applicant: BASF AG

Inventor： MATTES RALF ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  RESS-LOSCHKE MARION ,  ENGELS DIRK

IPC: C07H21/00 ,  C12N9/78 ,  C12N1/21 ,  C12N15/09 ,  C12N15/55 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：EE05008B1

公开(公告)日：2008-04-15

申请号：EEP200100232

申请日：1999-10-13

Applicant: BASF AG

Inventor： RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  HAUER BERNHARD ,  MATTES RALF ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.

公开(公告)号：DK1123386T3

公开(公告)日：2007-05-07

申请号：DK99952558

申请日：1999-10-13

Applicant: BASF AG

Inventor： MATTES RALF ,  HAUER BERNHARD ,  RESS-LOESCHKE MARION ,  FRIEDRICH THOMAS ,  ENGELS DIRK

IPC: C12N9/78 ,  C12N15/09 ,  C07H21/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/63 ,  C12P7/40 ,  C12P7/42 ,  C12P41/00 ,  C12R1/05

Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.