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    NUCLEIC ACID ENCODING REACTIONS
    22.
    发明专利
    NUCLEIC ACID ENCODING REACTIONS 未知

    公开(公告)号：SG194745A1

    公开(公告)日：2013-12-30

    申请号：SG2013081195

    申请日：2012-05-21

    Applicant: FLUIDIGM CORP

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  ANDREW MAY

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    23.
    发明专利
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 未知

    公开(公告)号：CA2874343A1

    公开(公告)日：2013-11-28

    申请号：CA2874343

    申请日：2013-05-21

    Applicant: FLUIDIGM CORP

    Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  KAPER FIONA ,  LEBOFSKY RONALD ,  MAY ANDREW

    IPC: C12Q1/68

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out "in parallel," i.e., essentially simultaneously in the separate reaction volumes.

    PROGRAMMABLE MICROFLUIDIC DIGITAL ARRAY
    24.
    发明专利
    PROGRAMMABLE MICROFLUIDIC DIGITAL ARRAY 未知

    公开(公告)号：SG172015A1

    公开(公告)日：2011-07-28

    申请号：SG2011041597

    申请日：2009-12-07

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN

    Abstract: A microfluidic device includes a pressure source and a control line in fluid communication with the pressure source. The microfluidic device also includes a plurality of valves operated via the control line and an independent valve positioned adjacent the control line and between the pressure source and the plurality of valves.

    INTEGRATED SINGLE CELL SEQUENCING
    29.
    发明专利
    INTEGRATED SINGLE CELL SEQUENCING 未知

    公开(公告)号：SG10202004286UA

    公开(公告)日：2020-06-29

    申请号：SG10202004286U

    申请日：2015-05-08

    Applicant: FLUIDIGM CORP

    Inventor： WEST JASON A A ,  FOWLER BRIAN ,  CHARN TZE-HOWE ,  JOHNSON CHRISTIAN F ,  UNGER MARC A

    Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    30.
    发明专利
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 未知

    公开(公告)号：SG10201608159XA

    公开(公告)日：2016-11-29

    申请号：SG10201608159X

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

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