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    PROBE FOR DIAGNOSIS OF INFECTIOUS DISEASE CAUSED BY KLEBSIELLA PNEUMONIAE
    21.
    发明专利
    PROBE FOR DIAGNOSIS OF INFECTIOUS DISEASE CAUSED BY KLEBSIELLA PNEUMONIAE 失效

    公开(公告)号：JPH10262677A

    公开(公告)日：1998-10-06

    申请号：JP7108297

    申请日：1997-03-25

    Applicant: FUSO PHARMACEUTICAL IND

    Inventor： KAMIYAMA HIROSHI ,  FUKUDA KANAKO ,  KARASHI HIROYUKI ,  MATSUHISA AKIO

    IPC: C12N15/09 ,  C07H21/04 ,  C12N15/00 ,  C12N15/31 ,  C12Q1/68 ,  C12R1/22 ,  G06F3/0354

    Abstract: PROBLEM TO BE SOLVED: To obtain the subject probe containing a DNA fragment produce by the restriction enzyme treatment of a DNA sequence of a specific microorganism and especially useful for the detection and identification of causal microorganisms of sepsis and Klebsiella pneumonia. SOLUTION: The subject microorganism is cultured under anaerobic condition, the DNA extracted from the cultured product is completely digested with HindIII and randomly cloned with vector pGEM-3Z and a probe containing a DNA sequence exhibiting specific reaction with the DNA of the subject microorganism is selected. The reactivity of each prove with the DNA of a clonic microbial strain is confirmed by dot blot hybridization and a DNA probe confirmed to have species specificity (e.g. a probe containing the base sequence of the formula) is collected. The causal microorganism of infectious diseases is quickly and accurately identified by this probe without culturing or proliferating the microorganism.

    TWO STAGE THERMOCYCLE PCR METHOD
    22.
    发明专利
    TWO STAGE THERMOCYCLE PCR METHOD 失效

    公开(公告)号：JPH0690756A

    公开(公告)日：1994-04-05

    申请号：JP24179892

    申请日：1992-09-10

    Applicant: FUSO PHARMACEUTICAL IND

    Inventor： KAMIYAMA HIROSHI ,  FUKUDA KANAKO ,  MATSUHISA AKIO

    IPC: C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C12N15/11

    Abstract: PURPOSE:To provide a PCR method more simple and accurate than the conventional method. CONSTITUTION:In the first stage, a sample containing a target sequence- containing double stranded DNA is subjected to a reaction for denaturating the above DNA at a temperature generally used for denaturation of a DNA in the conventional PCR method. In the second stage, in the presence of normal- and reverse-direction primers respectively having of a length of >=35 bases, four kinds of bases dGTP, dATP, dTTP and dCTP and DNA polymerase, an annealing reaction between both the primers for the above denaturated DNA containing the target sequence and an elongation reaction of the primer chain by DNA polymerase are carried out at a temperature generally used for elongation of a primer chain in the conventional PCR method. This two stage thermocycle PCR method is thus characteristic.

    DETECTION OF NUCLEIC ACID
    23.
    发明专利
    DETECTION OF NUCLEIC ACID 失效

    公开(公告)号：JPH0699A

    公开(公告)日：1994-01-11

    申请号：JP15934992

    申请日：1992-06-18

    Applicant: FUSO PHARMACEUTICAL IND

    Inventor： MATSUHISA AKIO ,  SHIBA KIYOTAKA ,  MIKAWA GIICHI ,  KISHI YUICHIRO

    IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/50 ,  G01N33/58

    Abstract: PURPOSE:To provide a method for detecting nucleic acid which is easy, safe, reliable and simple. CONSTITUTION:A solid carrier suspected to attach or contain nucleic acid is brought into contact with a polyamine to which a label (or its precursor) capable of giving measurable signals is bound, forming a composite made up of the nucleic acid and the polyamine. In case the precursor is used, it is transformed into the label. Before and after the transformation, the residual polyamine not forming the above composite is eliminated and then the label is probed, thus detecting the nucleic acid.

    30.
    发明专利
    未知

    公开(公告)号：DE69735562T2

    公开(公告)日：2006-08-17

    申请号：DE69735562

    申请日：1997-07-24

    Applicant: FUSO PHARMACEUTICAL IND

    Inventor： KESHI HIROYUKI ,  EDA SOJI ,  UEHARA HIROTSUGU ,  NISHIDA KEIGO ,  MATSUHISA AKIO

    IPC: C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12Q1/04 ,  C12R1/01

    Abstract: Probes for specifically detecting and identifying Helicobacter pylori which serves as an inflammatory bacterium in digestive diseases. These probes contain a fragment formed by completely digesting a DNA carried by H. pylori with a restriction enzyme HindIII. The information of the base sequences of these probes are useful as a structural indication of primers to be used in the construction of probes specific to H. pylori by PCR or as standard sequences suitable for the comparison with genomic DNAs contained in clinical specimens.

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