公开(公告)号：AU2003282750A1

公开(公告)日：2004-09-09

申请号：AU2003282750

申请日：2003-10-07

Applicant: INTEL CORP

Inventor： YAMAKAWA MINEO ,  CHAN SELENA ,  BERLIN ANDREW ,  KWON SUNGHOON ,  SUNDARARAJAN NARAYAN

IPC: B01D67/00 ,  B01D71/02 ,  C12Q1/68 ,  G01J3/44 ,  G01N21/65

Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.

公开(公告)号：AU2003278903A8

公开(公告)日：2004-04-19

申请号：AU2003278903

申请日：2003-09-24

Applicant: INTEL CORP

Inventor： KOO TAE-WOONG ,  SU XING ,  YAMAKAWA MINEO ,  CHAN SELENA ,  BERLIN ANDREW

IPC: C12Q1/68 ,  G01N33/543 ,  G01B7/34

Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

公开(公告)号：AU2003228627A1

公开(公告)日：2003-12-12

申请号：AU2003228627

申请日：2003-04-18

Applicant: INTEL CORP

Inventor： SUNDARARAJAN NARAYAN ,  BERLIN ANDREW ,  YAMAKAWA MINEO ,  SU XING ,  CHAN SELENA ,  KOO TAE-WOONG

IPC: G01N33/483 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/06 ,  G01N33/53

公开(公告)号：AT553380T

公开(公告)日：2012-04-15

申请号：AT04815440

申请日：2004-12-24

Applicant: INTEL CORP

Inventor： RAO VALLURI ,  MA QING ,  YAMAKAWA MINEO ,  BERLIN ANDREW ,  WANG LI-PENG ,  ZHANG YUEGANG

IPC: G01N33/543 ,  G01N29/02 ,  G01N29/036 ,  G01N29/34

公开(公告)号：AT453853T

公开(公告)日：2010-01-15

申请号：AT03774634

申请日：2003-10-07

Applicant: INTEL CORP

Inventor： CHAN SELENA ,  BERLIN ANDREW ,  KWON SUNGHOON ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

IPC: G01J3/44 ,  B01D67/00 ,  B01D71/02 ,  C12Q1/68 ,  G01N21/65

Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.

公开(公告)号：DE60316150T2

公开(公告)日：2008-06-05

申请号：DE60316150

申请日：2003-05-16

Applicant: INTEL CORP

Inventor： CHAN SELENA ,  BERLIN ANDREW ,  MINEO YAMAKAWA

IPC: G01J3/44 ,  C12Q1/68 ,  G01N21/65 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

Abstract: The methods and apparatus 300 disclosed herein concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates 240, 340 . In certain embodiments of the invention, porous silicon substrates 110, 210 may be formed by anodic etching in dilute hydrofluoric acid 150 . A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon 110, 210 by cathodic electromigration or any known technique. The metal-coated substrate 240, 340 provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain embodiments of the invention, metal nanoparticles may be added to the metal-coated substrate 240, 340 to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus 300.

公开(公告)号：AT372505T

公开(公告)日：2007-09-15

申请号：AT03760213

申请日：2003-05-16

Applicant: INTEL CORP

Inventor： CHAN SELENA ,  BERLIN ANDREW ,  MINEO YAMAKAWA

IPC: G01J3/44 ,  C12Q1/68 ,  G01N21/65 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

Abstract: The methods and apparatus 300 disclosed herein concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates 240, 340 . In certain embodiments of the invention, porous silicon substrates 110, 210 may be formed by anodic etching in dilute hydrofluoric acid 150 . A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon 110, 210 by cathodic electromigration or any known technique. The metal-coated substrate 240, 340 provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain embodiments of the invention, metal nanoparticles may be added to the metal-coated substrate 240, 340 to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus 300.

公开(公告)号：AU2003301375A1

公开(公告)日：2004-05-04

申请号：AU2003301375

申请日：2003-10-16

Applicant: INTEL CORP

Inventor： HAUSSECKER HORST ,  BERLIN ANDREW ,  CHAN SELENA ,  HANNAH ERIC ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

IPC: G01Q30/04 ,  G12B21/00 ,  G01N27/00

公开(公告)号：AU2003232139A1

公开(公告)日：2003-12-31

申请号：AU2003232139

申请日：2003-05-16

Applicant: INTEL CORP

Inventor： CHAN SELENA ,  BERLIN ANDREW ,  MINEO YAMAKAWA

IPC: C12Q1/68 ,  G01J3/44 ,  G01N21/65 ,  G01N33/53 ,  G01N33/543 ,  G01N33/553

Abstract: The methods and apparatus 300 disclosed herein concern Raman spectroscopy using metal coated nanocrystalline porous silicon substrates 240, 340 . In certain embodiments of the invention, porous silicon substrates 110, 210 may be formed by anodic etching in dilute hydrofluoric acid 150 . A thin coating of a Raman active metal, such as gold or silver, may be coated onto the porous silicon 110, 210 by cathodic electromigration or any known technique. The metal-coated substrate 240, 340 provides an extensive, metal rich environment for SERS, SERRS, hyper-Raman and/or CARS Raman spectroscopy. In certain embodiments of the invention, metal nanoparticles may be added to the metal-coated substrate 240, 340 to further enhance the Raman signals. Raman spectroscopy may be used to detect, identify and/or quantify a wide variety of analytes, using the disclosed methods and apparatus 300.

公开(公告)号：CA2478881A1

公开(公告)日：2003-09-25

申请号：CA2478881

申请日：2003-03-11

Applicant: INTEL CORP

Inventor： BERLIN ANDREW ,  SU XING ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO ,  CHAN SELENA ,  KOO TAE-WOONG

IPC: G01N21/65 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/68 ,  G01N33/543 ,  C07H21/02 ,  G01N21/29 ,  C12P19/34

Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing b y enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation int o a nucleic acid (13). Exonuclease (15) treatment of the labeled nucleic acid (13) results in the release of labeled nucleotides (16, 130), which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides (16, 130) released from a nucleic acid (13) by exonuclease (15) treatment are covalently cross-linked to silver or gold nanoparticles (140) and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus (1 0, 100, 210) for nucleic acid sequencing.