公开(公告)号：EP1045250A1

公开(公告)日：2000-10-18

申请号：EP99830209.5

申请日：1999-04-12

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Preda, Luigi

IPC: G01N33/86 ,  C12Q1/56

CPC classification number: C12Q1/56 ,  G01N33/86 ,  G01N2333/974

Abstract: The present invention relates to an analytical test for evaluating the functionality of the thrombin/antithrombin system.
In particular, the present invention relates to an analytical method for evaluating the functionality of the thrombin/antithrombin system, comprising the following steps:

(a) mixing a sample of plasma to be analyzed with an agent promoting the inhibitory activity of antithrombin;
(b) adding a Factor II activating agent to the mixture produced in step (a);
(c) measuring the time ta taken to convert the fibrinogen of the mixture produced in step (b) into fibrin.

Abstract translation: 本发明涉及评价凝血酶/抗凝血酶系统功能的分析试验。 具体而言，本发明涉及评价凝血酶/抗凝血酶系统功能的分析方法，包括以下步骤：（a）将待分析血浆样品与促进抗凝血酶抑制活性的试剂混合; （b）在步骤（a）中制备的混合物中加入因子II活化剂; （c）测量将步骤（b）中制备的混合物的纤维蛋白原转化为纤维蛋白的时间。

公开(公告)号：EP0830608B1

公开(公告)日：2000-10-04

申请号：EP96919107.1

申请日：1996-06-06

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： CAMPBELL, Paula, A. ,  PREDA, Luigi

IPC: G01N33/86 ,  C12Q1/56

CPC classification number: G01N33/86 ,  G01N2333/4626 ,  G01N2333/745 ,  G01N2333/96461 ,  G01N2333/96463 ,  G01N2405/04

公开(公告)号：EP0415471B1

公开(公告)日：1994-11-09

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

公开(公告)号：EP0528472A2

公开(公告)日：1993-02-24

申请号：EP92202344.5

申请日：1992-07-29

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Antocci, Joseph D. ,  Darling, Richard H. ,  Lawrence, Maxwell E., Jr. ,  Luongo, Joseph A.

IPC: G01N21/07 ,  G01N35/00

CPC classification number: G01N21/07 ,  Y10T436/111666 ,  Y10T436/113332 ,  Y10T436/25375

Abstract: A multicuvette rotor (10) which reduces unacceptable tendencies of reagent or a sample material to spontaneously move or "wick" from one chamber compartment (40) to the other (42), resulting in premature conmingling of reactants, and of sample or reagent material to flow out of one or more of the radially outer loading ports (18) during acceleration of the rotor for transfer of the sample or reagent material from inner chambers (40) to corresponding outer chambers (42).
Cover member disc (14) has planar lower surface (50) on which is formed a radially extending guide channel (56) adjacent each outer loading port (18); and an array of perimeter energy director ridges (60), each of which extends about the perimeter of an individual cuvette (30).

Abstract translation: 一种多单元转子，其减少试剂或样品材料的不可接受的趋势，以将其自发地从一个室隔室移动到“另一个室”，导致反应物过早混合，以及样品或试剂材料流出一个或多个 在加速转子期间将样品或试剂材料从内室转移到对应的外室的径向外加载端口。

公开(公告)号：EP0415471A3

公开(公告)日：1991-05-29

申请号：EP90202128.6

申请日：1990-08-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Frontini, Dario ,  D'Alterio, Maurizio

IPC: C12Q1/28

CPC classification number: C12Q1/28

Abstract: Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stablizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).

公开(公告)号：EP0358250A3

公开(公告)日：1990-09-12

申请号：EP89201949.8

申请日：1989-07-25

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Calzi, Claudio

IPC: G01N27/416

CPC classification number: G01N27/4163 ,  Y10T436/102499

Abstract: A pre-determined amount of solubilized reagent is reacted to release CO₂ in a controlled-temperature reaction vessel and a controlled-pressure area adjacent the reaction vessel. The devices that enable the system to be maintained in the liquid phase, and the reaction vessel itself, are controlled, assuming that the O₂ in the solution is such as that deriving from the presence of O₂ in the atmosphere, if necessary corrected for the effect of the reaction producing CO₂. Thus, the amount of reagent taking part in the reaction being known, either by assay or by controlling the reaction time, by controlling the amount of O₂ in the solution containing the reagent, the barometric pressure, the temperature in the reaction vessel, and the coefficients of solubility of the two gases, it becomes possible to predict the partial pressures of O₂ and CO₂ in the liquid phase produced, which liquid phase is suitable for use as a calibration substance for an apparatus for measuring blood gases.

公开(公告)号：EP0355896A3

公开(公告)日：1990-09-12

申请号：EP89202034.8

申请日：1989-08-03

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Calabrese, Gary Stephen ,  Calzi, Claudio

IPC: G01N27/416

CPC classification number: G01N27/4163

Abstract: Electrodes for the measurement of pCO2 and/or pO2 are calibrated with an exposed aliquot of a calibration liquid. A constituent such as a ferrioxalate salt in the calibration liquid is converted to the gas in a reproducible concentration by exposure of the aliquot to light. In some instances, the calibration liquid is equilibrated with air prior to exposure. Some mechanisms of light generation of carbon dioxide also consume oxygen, so as to depress the pO2 value by a reproducible amount. The use of two different calibration liquids enables both one-point and two-point calibration of the Clark oxygen electrode and the Severinghaus pCO2 electrode of a blood gas instrument. Aqueous acidic ferrioxalate compositions are disclosed for use as calibrants of pCO2 and of pO2 after photodecomposition. Compositions with high iron(III) to oxalate molar ratios (e.g., 5:1 to 100:1 with 0.3 to 15 millimolar oxalate) produce carbon dioxide on exposure without oxygen consumption. Compositions with low iron(III) to oxalate ratios (e.g., 1:1000 to 1:2000) with 0.1 to 5 millimolar iron(III) produce carbon dioxide on exposure with concurrent oxygen consumption. Use of the two types of compositions enables calibration values to be established with varying pCO2 values and with, respectively, high and low pO2 values.

公开(公告)号：EP0358250A2

公开(公告)日：1990-03-14

申请号：EP89201949.8

申请日：1989-07-25

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Calzi, Claudio

IPC: G01N27/416

CPC classification number: G01N27/4163 ,  Y10T436/102499

Abstract: A pre-determined amount of solubilized reagent is reacted to release CO₂ in a controlled-temperature reaction vessel and a controlled-pressure area adjacent the reaction vessel.
The devices that enable the system to be maintained in the liquid phase, and the reaction vessel itself, are controlled, assuming that the O₂ in the solution is such as that deriving from the presence of O₂ in the atmosphere, if necessary corrected for the effect of the reaction producing CO₂.
Thus, the amount of reagent taking part in the reaction being known, either by assay or by controlling the reaction time, by controlling the amount of O₂ in the solution containing the reagent, the barometric pressure, the temperature in the reaction vessel, and the coefficients of solubility of the two gases, it becomes possible to predict the partial pressures of O₂ and CO₂ in the liquid phase produced, which liquid phase is suitable for use as a calibration substance for an apparatus for measuring blood gases.

Abstract translation: 增溶的试剂的预确定的量进行反应在受控温度下的反应容器和一个受控压力区域相邻的反应容器，以释放CO 2。 设备确实使被保持在液相中的系统中，并且将反应容器本身被控制，假设没有象从O2的在大气中的存在推导O2在溶液中进行检查，如果需要校正的效果 的反应生产CO 2。 因此，试剂的参与反应的量是已知的，或者通过测定或通过控制反应时间，通过控制在溶液中的O 2的量的含有试剂，气压，在反应容器中的温度，并且 两种气体的溶解度系数，能够预测O2和CO2的分压在所产生的液体相，其液相是适合用作在设备中的校准物质用于测量血液气体。

公开(公告)号：EP0354604A2

公开(公告)日：1990-02-14

申请号：EP89201744.3

申请日：1989-06-29

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Calzi, Claudio ,  Premoli, Pietro

IPC: G01N27/416

CPC classification number: G01N27/4162

Abstract: A system is disclosed for measuring the partial pressure of a gas, and in particular of carbon dioxide (p CO ). The system uses an electrolytic solution of weak ionic strenght which is circulated between an ion-selective electrode and a membrane permeable to gases but not to the ions and substantially distant from the electrode. The partial pressure of the gas is measured after a fraction of the electrolytic solution has been permeated by the gas present in the sample and subsequently has been transferred on the sensible portion of the electrode.

Abstract translation: 一种系统是游离缺失盘用于测量气体的分压，和在二氧化碳（CO P）的具体。 该系统使用弱离子实力的电解液的所有其在离子选择性电极和膜透过气体但不与离子和从电极基本上遥远之间循环的。 后的电解液的一小部分已经通过样品中存在的气体透过，并随后已经转移上的电极的敏感部分的气体的局部压力被测量。

公开(公告)号：EP0299661A2

公开(公告)日：1989-01-18

申请号：EP88306089.9

申请日：1988-07-04

Applicant: INSTRUMENTATION LABORATORY S.p.A.

Inventor： Geiselman, Theodore S. ,  Rasmussen, James

IPC: G01N35/08

CPC classification number: G01N35/085

Abstract: A liquid handling system includes flow network module structure that defines a contained array of flow channels and a plurality of valves for controlling liquid flow through the flow channel array. The flow network module structure is adapted to be connected to an external source for applying a pressure differential to the flow network array to produce liquid flow within passages of the array. Also incorporated in the flow network module is chamber structure that is connected to the flow channel array and that has port structure in an outer surface of the module structure. Valve structure on the module structure is movable between a first position in which the port structure is closed and a second position in which the port structure is opened, the valve structure including actuator structure for moving a valve member between the first and second positions. Liquid transfer structure, including transport structure and probe structure carried on the transport structure, is adapted to cause movement of the valve structure from its first position to its second position concurrently with the movement of the probe structure into alignment with the chamber port structure for delivery of a quantity of sample material to the sample chamber and subsequent flow through the flow network array for interaction with an auxiliary fluid and transfer to an associated utilization device under the influence of an external pressure source.

Abstract translation: 液体处理系统包括流网络模块结构，其限定流体通道阵列和用于控制通过流动通道阵列的液体流的多个阀。 流网络模块结构适于连接到外部源，用于向流动网络阵列施加压差以在阵列的通道内产生液体流。 还包括在流动网络模块中的腔室结构连接到流动通道阵列并且在模块结构的外表面中具有端口结构。 模块结构上的阀结构可在端口结构关闭的第一位置和端口结构打开的第二位置之间移动，该阀结构包括用于在第一位置和第二位置之间移动阀构件的致动器结构。 液体转移结构，包括运输结构上承载的输送结构和探针结构，适于引起阀结构从其第一位置到其第二位置的运动，同时探针结构的运动与腔室结构对准以进行输送 的一定量的样品材料到样品室，随后流过流动网络阵列以与辅助流体相互作用并在外部压力源的影响下转移到相关联的利用装置。