coagulans ) KM-1, 이를 이용한 발효대두박 및 양어용 사료에 관한 것으로, 보다 상세하게는 알파-갈락토시다제 효소활성을 가지며 난소화성 탄수화물 분해활성이 우수한 신규한 미생물 바실러스 코아귤란스 KM-1 균주를 이용하여 대두박을 고온발효시킴으로써 기존의 대두박보다 소화율이 향상된 양어용 사료를 제조하는 방법에 관한 것이다.
Abstract:
To provide an economical and effective method for discerning pacific oyster triploids, the present invention provides a multiplex kit for discerning pacific oyster triploids, wherein the multiplex kit comprises three or more primer sets selected from the group including: a primer set 1 which consists of a forward primer having a nucleic acid sequence represented by sequence number 1 and a reverse primer represented by sequence number 2; a primer set 2 which consists of a forward primer having nucleic acid sequence represented by sequence number 5 and a reverse primer having a nucleic acid sequence represented by sequence number 6; a primer set 3 which consists of a forward primer having a nucleic acid sequence represented by sequence number 7 and a reverse primer having a nucleic acid sequence represented by sequence number 8; a primer set 4 which consists of a forward primer having a nucleic acid sequence represented by sequence number 15 and a reverse primer having a nucleic acid sequence represented by sequence number 16; a primer set 5 which consists of a forward primer having a nucleic acid sequence represented by sequence number 19 and a reverse primer having a nucleic acid sequence represented by sequence number 20; and a primer set 6 which consists of a forward primer having a nucleic acid sequence represented by sequence number 21 and a reverse primer having a nucleic acid sequence represented by sequence number 22.
Abstract:
The present invention relates to a peptide representing serine protease inhibition activity and firstly separated from Fenneropenaeus chinensis, and proved anti-trypsin activity and anticoagulant activity. According to an embodiment of the present invention, a novel serine protease inhibitor is separated from Fenneropenaeus chinensis and identified, and the present invention proves effects of anti-trypsin activity and anticoagulant activity thereof, thereby efficiently applying the same as an active ingredient of anticoagulants. [Reference numerals] (AA) Anticoagulant activity(Prolongation of APTT(s))
Abstract:
PURPOSE: A DNA hybridization detection method using gold nanoparticles is provided to enhance speed, accuracy, and sensitivity of DNA hybridization detection and to reduce time and cost due to reduced reaction time. CONSTITUTION: A DNA hybridization detection method comprises: a step of reacting target DNA conjugated with gallic acid to a probe DNA to induce hybridization; a step of removing the target DNA to which unhybridized gallic acid is conjugated; and a step of adding gold ions to the probe DNA which was hybridized with the target DNA and measuring the generation of gold nanoparticles.
Abstract:
The present invention relates to an EP feedstuff composition for halibuts which can be a substitute for a moisture pellet (MP) feedstuff. A protein base material of the feedstuff composition is adjusted so that the feedstuff composition can have a low feed coefficient while retaining nutrients a moisture pellet (MP) feedstuff has. The feedstuff composition is manufactured by mixing 66 weight% of brown fish powder, 5 weight% of hydrolyzed fish protein, 1 weight% of krill powder, 1 weight% of squid powder, 2 weight% of a fermented soybean meal, 1 weight% of concentrated soybean protein, 2 weight% of wheat gluten, 16 weight% of wheat flour, 3 weight% of fish oil, and 3 weight% of inorganic materials and additives. When compared to the moisture pellet (MP) feedstuff, the EP feedstuff composition has similar breeding results of growing and fattening halibuts and has similar feedstuff efficiency. Meanwhile, the EP feedstuff has an outstandingly low feed coefficient and thus can contribute to development of an aquaculture industry.
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Abstract:
PURPOSE: A genetic method for distinguishing colour variant in Stichopus japonicus Selenka and to improve accuracy of distinguishing. CONSTITUTION: A genetic method for distinguishing colour variant Stichopus japonicus Selenka using allele-specific PCR comprises: a step of isolating genome DNA of HSP70 from variants in Stichopus japonicus Selenka; a step of adding a primer set of sequence numbers 2 and 3 and an allele-specific primer of sequence number 4 to the isolated DNA for performing PCR; and a step of determining the species. The primer of sequence number 4 is used for allele-specific PCR amplification to distinguish the color of Stichopus japonicus Selenka.
Abstract translation:目的:区分ic ishing ishing ishing ishing ishing ishing。。。。。。。。。。。。。。。。。。。。。。。 构成:使用等位基因特异性PCR区分色变St us us A A A A A A A A A;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;; 将序列号2和3的引物组和序列号4的等位基因特异性引物加入分离的DNA进行PCR的步骤; 并确定物种的一个步骤。 序列号4的引物用于等位基因特异性PCR扩增,以区分日本j the的颜色。