Abstract:
본 발명은 안데스감자반점바이러스 진단용 프라이머 세트 및 이의 용도에 관한 것으로, 본 발명에 따른 APMoV 진단용 올리고뉴클레오티드 프라이머 세트를 이용한 RT-PCR을 통해 APMoV를 진단하는 방법은 항혈청을 이용한 진단법에 비해 1000배 이상 정확하고 빠르게 APMoV 감염 여부를 확인할 수 있으며, 가지과 작물에서 지금까지 알려진 모든 계통의 APMoV에 대한 검출이 가능하므로 검역 현장에서 유용하게 이용될 수 있을 것이다.
Abstract:
The present invention relates to an oligonucleotide probe set to specifically diagnose or detect Pepino mosaic virus (PepMV), genechips comprising oligonucleotide probe set to specifically diagnose and detect the PepMV, a method to specifically diagnose or detect the PepMV using the oligonucleotide probe set, and a kit comprising the oligonucleotide probe set and a hybridization solution to specifically diagnose or detect the PepMV. Since the PepMV is specifically detectable using the probe set of the present invention, the probe set is expected to be useful to increase the production of domestic agricultural products by minimizing the viral diseases of the domestic agricultural products.
Abstract:
The present invention relates to an oligonucleotide probe set to specifically diagnose or detect Eggplant mottled dwarf virus (EMDV), genechips comprising the oligonucleotide probe set to specifically diagnose and detect the EMDV, a method to specifically diagnose or detect the EMDV using the oligonucleotide probe set, and a kit comprising the oligonucleotide probe set and a hybridization solution to specifically diagnose or detect the EMDV. Since the EMDV is specifically detectable using the probe set of the present invention, the probe set is expected to be useful to increase the production of domestic agricultural products by minimizing the viral diseases of the domestic agricultural products.
Abstract:
The present invention relates to a method for detecting and distinguishing a beet curly top virus, a beet severe curly top virus, and a beet mild curly top virus which are viruses of the genus curtovirus using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). More specifically, the present invention relates to a method for detecting and distinguishing each of the viruses by confirming pieces after treating Dra I, which is a restriction enzyme, on a byproduct that is amplified using a primer set for PCR that can amplify all of the three viruses. By means of the method according to the present invention, three species of the curtovirus can be effectively diagnosed and distinguished within a short period of time without high-priced technical equipment such as sequence analysis equipment. The species are not reported to have occurred in Korea but the viruses often occur in major agricultural importers including North America. Accordingly, the curtovirus that can severely damage farms is rapidly and accurately detected in a preemptive plant quarantine step so that a huge economical loss can be remarkably reduced.
Abstract:
PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.
Abstract:
본발명은클라비박터미시가넨시스아종네브라스켄시스를특이적으로진단하기위한프라이머세트및 이의용도에관한것으로, 본발명에서는총 5개의프라이머세트를이용하여검역세균인클라비박터미시가넨시스아종네브라스켄시스(subsp.)를특이적으로진단할수 있는것을확인하였다. 따라서, 본발명을통해클라비박터미시가넨시스아종네브라스켄시스에대한특이적진단이쉽고빠르게수행될수 있으므로, 클라비박터미시가넨시스아종네브라스켄시스균주의국내로의도입을철저히방지할수 있어, 매우유용하다.