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31.发明授权벼 유래의 오에스엘아이엠 유전자, 상기 유전자를 포함하는발현벡터, 상기 발현벡터로 형질전환된 형질전환체 및 상기 형질전환체를 제조하는 방법 有权从Oryzasativa分离的OSLIM基因，含有基因的表达载体，载体转化的转化体和转化体的方法
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公开(公告)号：KR1020100127598A
公开(公告)日：2010-12-06
申请号：KR1020090046105
申请日：2009-05-26
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for enhancing target protein accumulation by suppressing storage protein expression of a plant is provided to effectively suppress glutelin gene group. CONSTITUTION: A polynucleotide having RNAi in a plant contains a nucleotide sequence of sequence number 1. The glutelin expression is suppressed by introducing a recombinant expression vector having the polynucleotide with nucleotide of sequence number 1 and a promoter to a plant. A method for enhancing the target protein accumulation comprises: a step of introducing the recombinant expression vector to the plant; and a step of introducing the target gene to the plant.
Abstract translation: 目的：提供通过抑制植物的储存蛋白表达来增强靶蛋白积累的方法，以有效抑制谷蛋白基因组。 构成：植物中具有RNAi的多核苷酸含有序列号1的核苷酸序列。通过将具有序列号1的核苷酸的多核苷酸和启动子的重组表达载体导入植物来抑制谷蛋白的表达。 增强靶蛋白质积累的方法包括：将重组表达载体导入植物的步骤; 以及将靶基因引入植物的步骤。
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公开(公告)号：KR1020100125523A
公开(公告)日：2010-12-01
申请号：KR1020090044281
申请日：2009-05-21
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A plant transformation method using a carrier and a transformant are provided to be widely used as a core technique in biological technology. CONSTITUTION: A carrier for transformation contains at least two restriction enzyme cleavage sites which are cleaved to protrude at least one T base at 3' in order to insert a PCR product. The carrier contains at least two restriction enzyme cleavage sites which are cleaved to protrude a T base at 3' in order to insert a PCT product between a promoter and a terminator. The restriction enzyme is AhdI, Eam1105, BciVI, BmrI, HphI, Hpy188I, HpyCH4III, or MboII. The cleavage site is 5'-CTTGG-3' or 5'-CCTGG-3'. A transformant is prepared by transforming a host cell with the carrier.
Abstract translation: 目的：使用载体和转化体的植物转化方法被广泛用作生物技术的核心技术。 构成：用于转化的载体含有至少两个限制酶切割位点，其被切割以在3'处突出至少一个T碱基以插入PCR产物。 载体含有至少两个限制酶切割位点，其在3'处被切割以突出T碱基，以便在启动子和终止子之间插入PCT产物。 限制酶是AhdI，Eam1105，BciVI，BmrI，HphI，Hpy188I，HpyCH4III或MboII。 切割位点为5'-CTTGG-3'或5'-CCTGG-3'。 通过用载体转化宿主细胞来制备转化体。
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公开(公告)号：KR101696681B1
公开(公告)日：2017-01-17
申请号：KR1020150105781
申请日：2015-07-27
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은무름병저항성이증진된형질전환식물체에관한것으로, 보다구체적으로 BrWRKY7 유전자를포함하는형질전환용벡터로식물세포를형질전환하여 BrWRKY7 유전자를과발현시켜무름병에대해높은저항성을가지는형질전환식물체에관한것이다. 본발명의형질전환식물체를이용하면농약사용절감이가능하고그로인해생산성향상및 안전한농산물공급이가능하게될 것이다.
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Patent Agency Ranking
公开(公告)号：KR101510252B1
公开(公告)日：2015-04-10
申请号：KR1020130139245
申请日：2013-11-15
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은배추유래광 파장특이적 BrEXPA1 프로모터및 이의용도에관한것으로, 구체적으로배추로부터분리한 BrEXPA1(brassica rapa a-expansin1) 프로모터는목적유전자를식물의전체조직에서발현시키면서동시에광 파장에따라발현이달라지므로 LED 광원의다양한광 파장을이용한작물의성장조절에활용할수 있다.
Abstract translation: 本发明涉及光特异性BrEXPA1启动子及其用途。 更具体地说，与芸苔属芜菁分离的芸苔属rapa a-expansin1（BrEXPA1）启动子在植物的整个组织中表达靶基因。 同时，启动子的基因表达根据光波长而变化。 因此，光特异性BrEXPA1启动子可用于通过使用LED光源的各种光波长来调节植物的生长。
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公开(公告)号：KR1020140070711A
公开(公告)日：2014-06-11
申请号：KR1020120131907
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/8223 , C07K14/415 , C12N15/8205 , C12N15/823 , C12N15/8251 , C12N15/8257
Abstract: The present invention relates to a BrSTY1 promoter consisting of a nucleotide sequence of SEQ ID NO: 1 and specifically inducing expression in stem apical meristem, leaves, flowers, and pods, and more specifically, to a promoter inducing an expression specifically in stem apical meristem, leaves, flowers, and pods, a recombinant expression vector comprising the same, and a plant transformed with the recombinant expression vector. A BrSTY1 promoter of the present invention induces expression specifically in stem apical meristem, leaves, flowers, and pods thereby specifically expressing a target protein by using the promoter to improve traits of crops.
Abstract translation: 本发明涉及由SEQ ID NO：1的核苷酸序列组成的BrSTY1启动子，并特异性诱导在茎尖分生组织，叶，花和荚中的表达，更具体地说，涉及在茎顶端分生组织中特异性诱导表达的启动子 ，叶，花和荚果，包含其的重组表达载体和用重组表达载体转化的植物。 本发明的BrSTY1启动子诱导特异性在茎顶分生组织，叶，花和荚中的表达，从而通过使用促进剂来改善作物的性状来特异性表达靶蛋白。
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公开(公告)号：KR101303806B1
公开(公告)日：2013-09-09
申请号：KR1020110096920
申请日：2011-09-26
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 식물의 특정한 생장 발달 시기에 특정한 조직에서 유전자의 발현을 특이적으로 유도할 수 있는 신규한 프로모터에 관한 것이다. 보다 구체적으로, 본 발명은 식물의 개화 및 종자 성숙 시기에 꽃 조직인 화탁과 꽃잎, 꽃받침, 꽃실의 기저부 등에서 유전자의 발현을 특이적으로 유도할 수 있는 신규한 BrCYS7 프로모터에 관한 것이다.
본 발명에 따르면 식물의 개화 및 종자 성숙 시기에 꽃 조직인 화탁과 꽃잎, 꽃받침, 꽃실의 기저부, 암술대 및 꼬투리의 말단에서 유전자의 발현을 특이적으로 유도할 수 있는 형질전환 식물을 제조할 수 있다. 따라서 식물의 생장 발달 단계 중 상기 특정 조건 하에서 유전자 생성물의 효율적인 생산을 위해 유용하게 활용될 수 있다.-
公开(公告)号：KR1020130033076A
公开(公告)日：2013-04-03
申请号：KR1020110096920
申请日：2011-09-26
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/823 , A01H4/008 , C07K14/415 , C12N15/8205
Abstract: PURPOSE: A BrCYS7 promoter which is specific to receptable is provided to specifically induce gene expression in a certain tissue, and to effectively produce a gene product. CONSTITUTION: A promoter contains a nucleic acid sequence in sequence number 1. The promoter contains a nucleic acid sequence having 70% or more of sequence homology with the nucleic acid in sequence number 1. An expression vector contains the promoter, and is a pPBrCYS7 vector. A cell is transformed with the expression vector, and is an Agrobacterium sp. microorganism or a plant cell.
Abstract translation: 目的：提供特异性诱导的BrCYS7启动子，以特异性诱导特定组织中的基因表达，并有效产生基因产物。 构成：启动子含有序列号1中的核酸序列。启动子含有与序列号1中的核酸具有70％以上序列同源性的核酸序列。表达载体含有启动子，是pPBrCYS7载体 。 用表达载体转化细胞，是农杆菌属。 微生物或植物细胞。
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公开(公告)号：KR101120406B1
公开(公告)日：2012-06-27
申请号：KR1020090046105
申请日：2009-05-26
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for enhancing target protein accumulation by suppressing storage protein expression of a plant is provided to effectively suppress glutelin gene group. CONSTITUTION: A polynucleotide having RNAi in a plant contains a nucleotide sequence of sequence number 1. The glutelin expression is suppressed by introducing a recombinant expression vector having the polynucleotide with nucleotide of sequence number 1 and a promoter to a plant. A method for enhancing the target protein accumulation comprises: a step of introducing the recombinant expression vector to the plant; and a step of introducing the target gene to the plant.
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