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    밀리타리스 동충하초 동정방법
    33.
    发明公开
    밀리타리스 동충하초 동정방법 有权
    识别脊髓灰质炎的方法

    公开(公告)号：KR1020140048379A

    公开(公告)日：2014-04-24

    申请号：KR1020120112845

    申请日：2012-10-11

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 김기영 ,  홍인표 ,  김미자 ,  강필돈 ,  성규병 ,  류강선 ,  김현복 ,  김용순 ,  지상덕 ,  김남숙 ,  김영기

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/686 ,  C12Q2525/151

    Abstract: The present invention relates to an identifying method of Cordyceps militaris using a PCR method; a primer used for the method; and a Cordyceps militaris identifying kit including the primer. By using the method of the present invention, a user can accurately and rapidly identify Cordyceps militaris which is not easily identified by the outer appearance, thereby capable of being widely applied for developing various products containing Cordyceps militaris.

    Abstract translation: 本发明涉及使用PCR方法鉴定冬虫夏草的方法; 用于该方法的底漆; 和含有引物的冬虫夏草militaris鉴定试剂盒。 通过使用本发明的方法，用户可以准确而快速地鉴定出不易被外观识别的冬虫夏草，从而可广泛应用于开发含有冬虫夏草的各种产品。

    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질
    34.
    发明授权
    누에 혈림프 유래 단백질의 대량 정제방법 및 이를 통해 정제된 단백질 有权
    使用丝朊蛋白和纯化蛋白质的蛋白质的质量纯化方法

    公开(公告)号：KR101346057B1

    公开(公告)日：2013-12-31

    申请号：KR1020120068585

    申请日：2012-06-26

    Applicant: 대한민국(농촌진흥청장) ,  차의과학대학교 산학협력단

    Inventor： 이희삼 ,  조유영 ,  권해용 ,  남성희 ,  류강선 ,  강필돈 ,  박광영 ,  김미자 ,  최용수 ,  김호진 ,  황정욱

    IPC: C07K1/14 ,  C07K14/435 ,  C12N1/04

    Abstract: The present invention relates to a mass purification method of a silkworm hemolymph-derived protein and a protein purified thereby. The mass purification method according to the present invention comprises a first step of freeze-drying silkworm to prepare a silkworm dried material; a second step of removing silk gland and obtaining the remainder hemolymph; a third step of crushing the hemolymph and adding water thereto to prepare a hemolymph lysate; a fourth step of subjecting the hemolymph lysate to centrifugation to obtain a supernatant; and a fifth step of subjecting the supernatant to column chromatography to isolate a protein of 70kDa. According to the present invention, the silkworm hemolymph is applicable for several uses, such as a cell cryopreservative, a medium supplement for cell culture, and the like. Especially, the ingredients remaining after the isolation of silkworm hemolymph are also reusable, so that the present invention contributes to an increase in the income of sericulture farmers. [Reference numerals] (AA) Silk gland removal;(BB) Grown-up silkworm;(CC) live body weight 3.13g;(DD) Centrifugation;(EE) Quick freezing;(FF) Grown-up silkworm freeze-drying 590 mg;(GG) pulverization/selection;(HH) hemolymph 380 mg;(II) Dissolution (distilled water);(JJ) 63 mg after heat treatment;(KK) 30k 70k separation;(LL) Final retrieval amount

    Abstract translation: 本发明涉及一种蚕血淋巴衍生蛋白的质量纯化方法和由此纯化的蛋白质。 根据本发明的质量净化方法包括：冷冻干燥蚕的第一步骤，制备蚕干物质; 去除丝腺并获得剩余血淋巴的第二步; 破碎血淋巴并向其中加入水以制备血淋巴溶胞产物的第三步骤; 第四步，使血淋巴溶胞产物离心得到上清液; 和将上清液进行柱色谱分离70kDa的蛋白质的第五步骤。 根据本发明，蚕血淋巴可应用于细胞冷冻保存剂，细胞培养用培养基等的多种用途。 特别是在分离蚕血淋淋后残留的成分也是可重复利用的，因此本发明有助于增加蚕食农民的收入。 （参考号）（AA）丝腺去除;（BB）成熟蚕;（CC）活体重3.13g;（DD）离心;（EE）快速冷冻;（FF）成熟蚕冷冻590 （GG）粉碎/选择;（HH）血淋巴380mg;（II）溶解（蒸馏水）;（JJ）热处理后63mg;（KK）30k 70k分离;（LL）最终检索量

    실크 펩타이드를 활성성분으로 함유하는 골다공증 예방 및치료용 조성물
    39.
    发明公开
    실크 펩타이드를 활성성분으로 함유하는 골다공증 예방 및치료용 조성물 有权
    用于预防和治疗包含丝胶蛋白作为活性成分的卵泡病的组合物

    公开(公告)号：KR1020090036758A

    公开(公告)日：2009-04-15

    申请号：KR1020070101980

    申请日：2007-10-10

    Applicant: 대한민국(농촌진흥청장) ,  동성제약주식회사

    Inventor： 여주홍 ,  이광길 ,  권해용 ,  우순옥 ,  한상미 ,  강필돈 ,  박경호 ,  이범구

    IPC: A61K35/64 ,  A61K38/01

    CPC classification number: A61K35/63 ,  A61K38/012

    Abstract: A composition for preventing and treating osteoporosis comprising silk peptide as an active ingredient is provided to suppress the differentiation of osteoclast by using natural protein. A composition for preventing and treating osteoporosis comprises silk peptide as an active ingredient. The silk peptide is an acid of silk or hydrolysate of enzyme. The silk peptide is the pyrolysate of cocoon containing sericin. An amino acid composition of the silk peptide are 80 weight% or greater based on the total content of glycine 40-45 weight%, alanine 20-30 weight% and serine 10-20 weight%.

    Abstract translation: 提供一种用于预防和治疗包含丝肽作为活性成分的骨质疏松症的组合物，以通过使用天然蛋白质来抑制破骨细胞的分化。 用于预防和治疗骨质疏松症的组合物包括丝肽作为活性成分。 丝肽是丝的酸或酶的水解产物。 丝肽是含有丝胶蛋白的茧的热解物。 基于甘氨酸40-45重量％，丙氨酸20-30重量％和丝氨酸10-20重量％的总含量，丝肽的氨基酸组成为80重量％以上。

    인터론영역에서 직접염기서열분석에 의한 누에분말 판별법
    40.
    发明公开
    인터론영역에서 직접염기서열분석에 의한 누에분말 판별법 无效
    通过直接序列分析区域内的丝状丝菌株的鉴定

    公开(公告)号：KR1020090032487A

    公开(公告)日：2009-04-01

    申请号：KR1020070097789

    申请日：2007-09-28

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 김기영 ,  강필돈 ,  김익수 ,  정이연 ,  손봉희 ,  김미자 ,  강석우

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  C12Q1/686

    Abstract: Provided is a method for discriminating domestic silkworm powder from imported silkworm powder through direct base sequencing analysis. Nine introns are used as silkworm discrimination markers. The nine introns indicate A4Intron1, A4Intron2, PTTHIntron3, LCP30Intron3, LCP30Intron4, SPIIntron3, VDPIntron4, FLCIntron3 and XDHIntron3. A method for discriminating domestic silkworm powder using the direct base sequencing analysis comprises the following steps of: extracting a genome DNA from the silkworm powder; offering information about DNA amplification existence or nonexistence in a specific intron region through PCR(polymerase chain reaction); offering information about discriminable intron regions based on species and primer in the regions through the direct base sequencing analysis; and comparing the species in the intron regions using the silkworm discrimination marker.

    Abstract translation: 提供了一种通过直接碱基测序分析鉴别家蚕粉与进口蚕粉的方法。 九个内含子用作蚕鉴别标记。 九个内含子表示A4Intron1，A4Intron2，PTTHIntron3，LCP30Intron3，LCP30Intron4，SPIIntron3，VDPIntron4，FLCIntron3和XDHIntron3。 使用直接碱基测序分析鉴别家蚕粉的方法包括以下步骤：从蚕粉中提取基因组DNA; 通过PCR（聚合酶链反应）提供有关DNA扩增存在或不存在于特定内含子区域的信息; 通过直接碱基测序分析，提供区域内基于物种和引物的可区分内含子区域的信息; 并使用家蚕鉴别标记物比较内含子区域中的物种。

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