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公开(公告)号：KR1020170079550A
公开(公告)日：2017-07-10
申请号：KR1020150190252
申请日：2015-12-30
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: G01N33/569 , G01N33/564 , C12N7/00
Abstract: 본발명은말 게타바이러스혈청진단방법에관한것으로, 구체적으로게타바이러스(Getah virus) KCTC18436P 항원을사용하여말의시료에존재하는게타바이러스항체를효율적으로검출할수 있는말 게타바이러스혈청진단방법및 이를위한말 게타바이러스혈청진단용키트에관한것이다. 본발명에따르면말에서게타바이러스에대한항체를신속하고정확하게검출할수 있으며, 이러한방법을동시에많은시료에적용할수 있다. 또한고가의전문적인분석장치가없더라도손쉽게적용이가능하며기존방법에비해경제적이라는장점도있다. 본발명의진단방법또는키트를통해보다많은수의시료를신속하고정확하게검사할수 있게됨에따라말 개체군내에서게타바이러스의분포및 이동을모니터링하거나예방백신을개발하는데크게기여할수 있을것이라기대된다.
Abstract translation: 木屐的本发明端涉及病毒血清诊断方法，并且具体地木屐病毒（盖塔病毒）使用KCTC18436P抗原话，可以有效地检测木屐病毒血清诊断方法的最后的样品中存在的木屐病毒的抗体，和为此 本发明涉及用于马鞭球病毒血清学诊断的试剂盒。 根据本发明，可以在马中快速且准确地检测出针对ge病毒的抗体，并且该方法可以同时应用于许多样本。 此外，即使没有昂贵的专业分析仪也很容易应用，与现有方法相比，它更经济。 预计到能够有助于本发明大量的样品的通过快速的诊断方法或试剂盒比和监测病毒在马口木屐作为使你准确地测试或开发疫苗的分布和流动。
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公开(公告)号：KR1020170022035A
公开(公告)日：2017-03-02
申请号：KR1020150116483
申请日：2015-08-19
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12Q1/70
Abstract: 본발명은게타바이러스검출용프라이머세트에관한것으로, 구체적으로서열번호 1의염기서열을갖는프라이머및 서열번호 2의염기서열을갖는프라이머를포함하고있어, RT-PCR을통해게타바이러스의 E1 유전자일부를특이적으로증폭할수 있고, 결과적으로다른유사바이러스들로부터게타바이러스만을정확하게검출할수 있도록하는프라이머세트에관한것이다. 본발명의프라이머세트, 조성물또는키트를사용하여 RT-PCR을수행하면시료내에게타바이러스가매우적은농도로존재하더라도매우민감도높게검출할수 있으며, 다른유사바이러스가존재하더라도게타바이러스만을정확하게검출해낼 수있다. 또한통상의 RT-PCR 방법을적용할수 있어쉽게빠르게검출할수 있다는장점이있다.
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33.发明公开광견병 바이러스 유전자를 발현하는 재조합 아데노바이러스 및 이를 포함하는 광견병의 예방 또는 치료용 백신 조성물 有权重组腺病毒表达RABIES病毒基因和疫苗组合物，用于预防或治疗包括其的RABY
公开(公告)号：KR1020140017253A
公开(公告)日：2014-02-11
申请号：KR1020120083952
申请日：2012-07-31
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: A61K39/235 , A61K39/39 , A61P31/00
CPC classification number: A61K39/235 , C12N15/66 , Y10S424/818
Abstract: The present invention relates to a recombinant adenovirus expressing a rabies virus gene and a vaccine composition for preventing or treating rabies comprising the same. The recombinant adenovirus according to the present invention, which is a recombinant virus of nucleotide sequences coding G protein and N protein of the rabies virus isolated from the country, effectively expresses G protein and N protein of the rabies virus and has a proliferation ability suitable for the use as a vaccine strain, and thus is suitable in mass production thereof. Further, the present invention has improved stability as compared with the exiting vaccine and thus can be used for oral administration, and has excellent ability to defend against the rabies virus at the time of vaccination.
Abstract translation: 本发明涉及表达狂犬病病毒基因的重组腺病毒和用于预防或治疗包含该狂犬病病毒基因的狂犬病的疫苗组合物。 根据本发明的重组腺病毒，其是从该国分离的狂犬病病毒的编码G蛋白和N蛋白的核苷酸序列的重组病毒，有效表达狂犬病病毒的G蛋白和N蛋白，并具有适合于 作为疫苗株的用途，因此适合于大量生产。 此外，与现有的疫苗相比，本发明具有改善的稳定性，因此可以用于口服给药，并且在接种疫苗时具有优异的防御狂犬病病毒的能力。
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公开(公告)号：KR1020140004909A
公开(公告)日：2014-01-14
申请号：KR1020120072247
申请日：2012-07-03
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N7/01 , A61K39/12 , G01N33/569
Abstract: The present invention relates to the recombinant rabies virus rERAG333Glu and a vaccine composition containing the same for preventing rabies. The recombinant rabies virus rERAG333Glu according to the present invention is a recombinant virus strain where the 333rd amino acid of G protein is replaced by glutamic acid. When manufacturing a vaccine from the virus, the vaccine has an excellent amount of virus so as to be suitable for mass-production, and has improved stability compared with the existing vaccines so as to be used for oral administration. Therefore, it can produce effects of solving problems of the existing rabies virus vaccines and being used as an effective vaccine for preventing rabies virus.
Abstract translation: 本发明涉及重组狂犬病病毒rERAG333Glu和含有该疫苗组合物的用于预防狂犬病的疫苗组合物。 根据本发明的重组狂犬病毒rERAG333Glu是重组病毒株，其中G蛋白的第333位氨基酸被谷氨酸代替。 当从病毒制造疫苗时，疫苗具有优良的病毒量以便适合批量生产，并且与现有疫苗相比具有改善的稳定性以便用于口服给药。 因此，它可以产生解决现有狂犬病毒疫苗问题的作用，并被用作预防狂犬病毒的有效疫苗。
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公开(公告)号：KR1020110097199A
公开(公告)日：2011-08-31
申请号：KR1020100016909
申请日：2010-02-25
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , (주) 쓰리샤인
Abstract: 본 발명은 바이러스에 대한 필터의 효력평가장치 및 효력평가방법에 관한 것이다.
본 발명의 바이러스에 대한 필터의 효력평가장치는 외부로부터 유입되는 바이러스를 수용하는 제1무균상챔버와, 제1무균상챔버와 연통하게 설치되어 제1무균상챔버로부터 공기를 흡입하여 제1무균상챔버의 공기중의 바이러스량을 검출하도록 한 제1포집부로 이루어진 제1검출수단; 상기 제1검출수단의 제1무균상챔버와 연통하는 제2검출수단의 제2무균상챔버와의 사이에 설치되어 제1무균상챔버로부터 제2무균상챔버로 유동하는 공기를 필터링하여 바이러스를 걸러내도록 한 필터수단; 및 상기 필터수단을 통해서 제1무균상챔버와 연통하게 설치되며 필터수단에 의해 필터링된 제1무균상챔버의 공기를 유입하여 수용하도록 한 제2무균상챔버와, 제2무균상챔버의 공기를 흡입함으로써 제1무균상챔버로부터 필터수단을 경유한 공기중의 바이러스를 포집하여 제2무균상챔버의 공기중의 바이러스량을 검출하도록 한 제2포집부로 이루어진 제2검출수단을 포함하여 구성된다.
본 발명에 의해, 다양한 바이러스에 대한 필터의 효력평가가 가능하고 음압공조기에 의해 생체인 실험동물을 대상으로 과학적이고 손쉽게 필터의 효력평가도 가능하여 편리한 효과가 있다.-
Patent Agency Ranking
公开(公告)号：KR1020110091284A
公开(公告)日：2011-08-11
申请号：KR1020100011044
申请日：2010-02-05
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: PURPOSE: A scFv gene of monoclonal antibody 4G31 and a recombinant protein expressing the same are provided to neutralize RAV and to reduce and diagnose rabies virus. CONSTITUTION: A 4G31 scFv(single chain variable fragment antibody) gene which specifically neutralizes rabies virus has an amino acid sequence and a base of sequence number 16. The 4G31 scFv gene is a 4G31 monoclonal antibody containing a gene encoding a heavy chain variable region(VH) and a light chain variable region(VL). An expression vector expresses the 4G31 scFv gene. A recombinant protein is prepared by expressingthe 4G31 scFv gene in E.coli using the expression vector. A therapeutic agent for rabies virus contains the recombinant protein expressed from the 4G31 scFv gene.
Abstract translation: 目的：提供单克隆抗体4G31的scFv基因和表达其的重组蛋白，以中和RAV并减少和诊断狂犬病病毒。 构成：特异性中和狂犬病病毒的4G31 scFv（单链可变片段抗体）基因具有氨基酸序列和序列号16的碱基。4G31 scFv基因是含有编码重链可变区的基因的4G31单克隆抗体（ VH）和轻链可变区（VL）。 表达载体表达4G31 scFv基因。 通过使用表达载体在大肠杆菌中表达4G31scFv基因来制备重组蛋白。 狂犬病病毒的治疗剂含有从4G31 scFv基因表达的重组蛋白。
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37.发明授权
公开(公告)号：KR100834014B1
公开(公告)日：2008-06-02
申请号：KR1020060102854
申请日：2006-10-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: 본 발명은 모기를 매개로 소에서 유사산을 일으키는 소 아까바네병, 츄잔병 및 아이노 바이러스 감염증 예방을 위한 아까바네(K-9), 츄잔(YoungAM) 및 아이노(KSA9910) 바이러스 균주를 이용한 3종 혼합 바이러스 백신 및 그 제조방법에 관한 것으로서, 보다 상세하게는 국내 분리주인 아까바네(K-9), 츄잔(YoungAM) 및 아이노(KSA9910) 바이러스 균주를 불활화시킨 후 이들을 면역증강제와 함께 혼합하여 제조함으로써 3가지 바이러스를 한가지 예방약으로 예방이 가능하도록 제조한 3종 혼합 바이러스 백신 및 그 제조방법에 관한 것이다.
소 아까바네병, 아이노바이러스 감염증, 츄잔병, 3종 혼합불활화 백신-
公开(公告)号：KR1020080040942A
公开(公告)日：2008-05-09
申请号：KR1020060108942
申请日：2006-11-06
Applicant: 대한민국(농림축산식품부 농림축산검역본부장) , 주식회사 바이오노트
Abstract: A kit and a method for diagnosing a porcine epidemic diarrhea virus antigen are provided to diagnose the antigen conveniently and rapidly without a special testing equipment in a farm or a laboratory field. A kit for diagnosing a porcine epidemic diarrhea virus antigen is characterized in that a conjugate of a monoclonal antibody 6G15(deposition no. KCTC 10996BP) coupled to nucleocapside 58kDa of an epidemic diarrhea virus and a gold particle is attached to a reaction position of the porcine epidemic diarrhea virus antigen on a strip and a monoclonal antibody 9D11(deposition no. KCTC 10997BP) is attached to a position different from the coupling portion of the porcine epidemic diarrhea virus antigen and the monoclonal antibody 6G15. A method for diagnosing the porcine epidemic diarrhea virus antigen comprises the steps of: (a) loading a sample onto a detection pad on a strip; (b) reacting the conjugate of the monoclonal antibody 6G15 coupled to nucleocapside 58kDa of the epidemic diarrhea virus and the gold particle with the porcine epidemic diarrhea virus antigen in the sample; and (c) reacting the porcine epidemic diarrhea virus antigen, the monoclonal antibody 9D11 and the reaction product obtained from the step(b).
Abstract translation: 提供了一种用于诊断猪流行性腹泻病毒抗原的试剂盒和方法，用于在农场或实验室领域中没有特殊检测设备的情况下方便快速地诊断抗原。 一种用于诊断猪流行性腹泻病毒抗原的试剂盒的特征在于，将与流行性腹泻病毒和金颗粒的核衣壳58kDa偶联的单克隆抗体6G15（沉积编号KCTC 10996BP）的缀合物连接到猪的反应位置 将条带上的流行性腹泻病毒抗原和单克隆抗体9D11（沉积编号KCTC 10997BP）连接到与猪流行性腹泻病毒抗原和单克隆抗体6G15的偶联部分不同的位置。 用于诊断猪流行性腹泻病毒抗原的方法包括以下步骤：（a）将样品加载到条带上的检测垫上; （b）将与流行性腹泻病毒的金卡帕58kDa和金颗粒偶联的单克隆抗体6G15的缀合物与样品中的猪流行性腹泻病毒抗原反应; 和（c）使猪流行性腹泻病毒抗原，单克隆抗体9D11和从步骤（b）获得的反应产物反应。
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39.发明公开아까바네, 츄잔, 아이노바이러스 균주 및 이를 이용한 혼합바이러스 백신의 제조방법 有权AKABANE VIRUS，CHUZAN VIRUS和AINO VIRUS的分离菌株，以及通过混合其制备病毒疫苗的方法
公开(公告)号：KR1020080036353A
公开(公告)日：2008-04-28
申请号：KR1020060102854
申请日：2006-10-23
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
Abstract: A viral vaccine containing isolated virus strains is provided to improve use safety and immunogenicity by using inactivated viruses, and increase production yield of cows, so that the vaccine is useful for prevention of abortion or stillbirth in cows caused by viruses via mediation of Culicoides spp. A viral vaccine for prevention of abortion or stillbirth in cows contains cultured medium of inactivated virus strains of akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P), and is produced by: inactivating akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P) by adding 0.1M BEI(binary ethylene imine) prepared by solubilizing 2-BEA(2-bromoethylamine) in 0.2N NaOH, and treating the solution in a constant temperature water bath of 37 deg. C for 1 hour to them; mixing the 3 kinds of viruses in a weight ratio of 1:1:1; and adding 40-60 wt.% of IMS 1314 as an immunomodulator into the viral mixture.
Abstract translation: 提供含有分离的病毒株的病毒疫苗，以通过使用灭活病毒提高使用安全性和免疫原性，并提高奶牛的产量，使得疫苗可用于通过Culicoides spp的调解来预防由病毒引起的奶牛的流产或死胎。 用于预防奶牛流产或死胎的病毒疫苗含有阿卡巴因病毒（K-9）（KCTC18117P），Chuzan病毒（KSA9910）（KCTC18118P）和Aino病毒（YoungCAM）（KCTC18119P）的灭活病毒株的培养基， 通过添加由0.1M BEI（二元乙烯亚胺）制备的阿卡巴因病毒（K-9）（KCTC 18117P），Chuzan病毒（KSA9910）（KCTC 18118P）和Aino病毒（YoungAM）（KCTC 18119P） 溶解在0.2N NaOH中的2-BEA（2-溴乙胺），并在37℃恒温水浴中处理该溶液。 C给他们1小时; 混合3种病毒，重量比为1：1：1; 并将40-60重量％的IMS 1314作为免疫调节剂加入到病毒混合物中。
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40.发明公开
公开(公告)号：KR1020080010771A
公开(公告)日：2008-01-31
申请号：KR1020060071258
申请日：2006-07-28
Applicant: 대한민국(농림축산식품부 농림축산검역본부장)
IPC: C12N15/12
Abstract: Recombinant proteins of enzootic bovine leukemia virus are provided to improve specificity and sensitivity of detection as compared with conventional antigens by expressing the envelope glycoproteins in baculovirus and secreting the envelope glycoproteins to insect cells. A method for producing a recombinant protein of enzootic bovine leukemia virus expressed in an insect cell comprises the steps of: (1) cloning an envelop glycoprotein of enzootic bovine leukemia virus from the blood of enzootic bovine leukemia positive cows to baculovirus and sequencing the cloned protein; (2) transforming an insect cell with a recombinant virus gene of SEQ ID NO:1 to express a recombinant protein of SEQ ID NO:2; and (3) purifying and concentrating the recombinant protein produced from the insect cell, wherein the recombinant virus gene of SEQ ID NO:1 is prepared by recombination of envelop glycoprotein genes including a whole gp51 gene and a part of gp30 gene.
Abstract translation: 通过在杆状病毒中表达包膜糖蛋白并将包膜糖蛋白分泌到昆虫细胞中，提供了特异性和灵敏度的检测与常规抗原的特异性和灵敏度。 用于生产在昆虫细胞中表达的特异性牛白血病病毒重组蛋白质的方法包括以下步骤：（1）将来自地方性牛白血病阳性牛的血液中的特异性牛白血病病毒的包膜糖蛋白克隆至杆状病毒，并测序克隆的蛋白质 ; （2）用SEQ ID NO：1的重组病毒基因转化昆虫细胞以表达SEQ ID NO：2的重组蛋白; （3）从昆虫细胞产生的重组蛋白质的纯化和浓缩，其中通过包括整个gp51基因和一部分gp30基因的包膜糖蛋白基因的重组制备SEQ ID NO：1的重组病毒基因。
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