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公开(公告)号:AU782517B2
公开(公告)日:2005-08-04
申请号:AU1141601
申请日:2000-10-20
Applicant: BASF AG
Inventor: KESSELER MARIA , HAUER BERNHARD , FRIEDRICH THOMAS , MATTES RALF
Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.
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公开(公告)号:BR0108883A
公开(公告)日:2003-04-29
申请号:BR0108883
申请日:2001-02-27
Applicant: BASF AG
Inventor: RESS-LDSCHKE MARION , HAUER BERNHARD , MATTES RALF , ENGELS DIRK
IPC: C12N15/09 , C12N1/21 , C12N9/78 , C12N15/55 , C12P7/40 , C12P41/00 , C12R1/19 , C12N15/70 , C12N15/76
Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).
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公开(公告)号:NO20021931L
公开(公告)日:2002-04-24
申请号:NO20021931
申请日:2002-04-24
Applicant: BASF AG
Inventor: KESSELER MARIA , HAUER BERNHARD , FRIEDRICH THOMAS , MATTES RALF
Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.
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公开(公告)号:NO20021931A
公开(公告)日:2002-04-24
申请号:NO20021931
申请日:2002-04-24
Applicant: BASF AG
Inventor: KESSELER MARIA , HAUER BERNHARD , FRIEDRICH THOMAS , MATTES RALF
CPC classification number: C12P17/04 , C12N9/18 , C12P41/005
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公开(公告)号:AU3380201A
公开(公告)日:2001-09-12
申请号:AU3380201
申请日:2001-02-27
Applicant: BASF AG
Inventor: RESS-LOSCHKE MARION , HAUER BERNHARD , MATTES RALF , ENGELS DIRK
IPC: C12N15/09 , C12N1/21 , C12N9/78 , C12N15/55 , C12P7/40 , C12P41/00 , C12R1/19 , C12N15/70 , C12N15/76
Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).
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公开(公告)号:AU1141601A
公开(公告)日:2001-05-14
申请号:AU1141601
申请日:2000-10-20
Applicant: BASF AG
Inventor: KESSELER MARIA , HAUER BERNHARD , FRIEDRICH THOMAS , MATTES RALF
Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.
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公开(公告)号:ES2280125T3
公开(公告)日:2007-09-01
申请号:ES99952558
申请日:1999-10-13
Applicant: BASF AG
Inventor: RESS-LOSCHKE MARION , FRIEDRICH THOMAS , HAUER BERNHARD , MATTES RALF , ENGELS DIRK
IPC: C12N9/78 , C12N15/09 , C07H21/00 , C12N1/21 , C12N15/55 , C12N15/63 , C12P7/40 , C12P7/42 , C12P41/00 , C12R1/05
Abstract: Una secuencia aislada de ácido nucleico que codifica para un polipéptido que tiene una actividad de nitrilasa, seleccionada del grupo de a) una secuencia de ácido nucleico que tiene representada en la SEQ ID NO: 1, b) secuencias de ácido nucleico que se derivan de la secuencia de ácido representada en la SEQ ID NO: 1 como un resultado de la degeneración del código genético. c) homólogos de la secuencia de ácido nucleico representada en la SEQ ID NO: 1, que codifican para polipéptidos que tienen al menos 98% de homología, por el rango entero de secuencia, a SEQ ID NO: 2, sin reducción en la acción enzimática de los polipéptidos.
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公开(公告)号:PT1123386E
公开(公告)日:2007-03-30
申请号:PT99952558
申请日:1999-10-13
Applicant: BASF AG
Inventor: HAUER BERNHARD , FRIEDRICH THOMAS , RESS-LOESCHKE MARION , MATTES RALF , ENGELS DIRK
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公开(公告)号:DE59914102D1
公开(公告)日:2007-02-08
申请号:DE59914102
申请日:1999-10-13
Applicant: BASF AG
Inventor: RESS-LOESCHKE MARION , FRIEDRICH THOMAS , HAUER BERNHARD , MATTES RALF , ENGELS DIRK
IPC: C12N9/78 , C12N15/09 , C07H21/00 , C12N1/21 , C12N15/55 , C12N15/63 , C12P7/40 , C12P7/42 , C12P41/00 , C12R1/05
Abstract: The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.
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公开(公告)号:AU2001233802B2
公开(公告)日:2006-01-12
申请号:AU2001233802
申请日:2001-02-27
Applicant: BASF AG
Inventor: MATTES RALF , RESS-LOSCHKE MARION , ENGELS DIRK , HAUER BERNHARD
IPC: C12N9/78 , C12N15/09 , C12N1/21 , C12N15/55 , C12N15/70 , C12N15/76 , C12P7/40 , C12P41/00 , C12R1/19
Abstract: Isolated nucleic acid (I), encoding a polypeptide (II) with nitrilase activity, is (i) a 1483 bp sequence (1), reproduced; (ii) a sequence equivalent to (1) within the degeneracy of the genetic code or (iii) a derivative of (1) that encodes a polypeptide having at least 95% homology with a 366 amino acid polypeptide (2), reproduced, and essentially the same enzymatic activity as (2). Independent claims are also included for the following: (a) (II) encoded by (I); (b) nucleic acid construct containing (I) linked to one or more regulatory signals; (c) vector containing (I) or the construct of (b); (d) recombinant microorganism containing (I), the construct of (b) or the vector of (c); and (e) method for producing achiral or chiral carboxylic acids (III) by treating nitriles (IV) with (II) or with growing, resting or lysed microorganisms of (d).
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