公开(公告)号：AR047058A1

公开(公告)日：2006-01-04

申请号：ARP040104742

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12P20060101 ,  C12P13/08 ,  C12N15/54 ,  C12N1/21 ,  C12R1/15

Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, glicerol quinasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de glicerol quinasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado.

公开(公告)号：CA2546847A1

公开(公告)日：2005-12-22

申请号：CA2546847

申请日：2004-12-17

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  ZELDER OSKAR ,  WITTMANN CHRISTOPH ,  HAFNER STEFAN

IPC: C12P13/08 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12P20060101

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2004320598A1

公开(公告)日：2005-12-22

申请号：AU2004320598

申请日：2004-12-17

Applicant: BASF AG

Inventor： WITTMANN CHRISTOPH ,  HEINZLE ELMAR ,  SCHRODER HARTWIG ,  ZELDER OSKAR ,  KIEFER PATRICK ,  KROGER BURKHARD ,  HAFNER STEFAN ,  KLOPPROGGE CORINNA

IPC: C12P13/08 ,  C12P20060101

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：DE10044132A1

公开(公告)日：2002-03-14

申请号：DE10044132

申请日：2000-09-06

Applicant: BASF AG

Inventor： HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: H01J49/04 ,  H01J49/16 ,  G01N27/64 ,  G01N33/483

Abstract: A method for qualitatively or quantitatively analyzing complex mixtures (A) of chemical compounds involves use of MALDI-TOF (matrix assisted laser desorption-ionization time-of-flight) mass spectrometry.

公开(公告)号：DE10027801A1

公开(公告)日：2001-12-13

申请号：DE10027801

申请日：2000-06-07

Applicant: BASF AG

Inventor： HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: H01J49/04 ,  G01N27/64 ,  G01N33/483

Abstract: A method for qualitatively or quantitatively analyzing complex mixtures (A) of chemical compounds involves use of MALDI-TOF (matrix assisted laser desorption-ionization time-of-flight) mass spectrometry.