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    31.
    发明专利
    未知

    公开(公告)号：ES2183563T3

    公开(公告)日：2003-03-16

    申请号：ES99925705

    申请日：1999-05-19

    Applicant: CEPHEID

    Inventor： CHRISTEL LEE A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A ,  YOUNG STEVEN J

    IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

    Abstract: An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel (2) having a chamber (10) for holding the mixture. The apparatus also includes a heat-exchanging module (37) having a pair of opposing thermal plates (34A, 34B) for receiving the vessel (2) between them and for heating and/or cooling the mixture contained in the vessel. The module (37) also includes optical excitation and detection assemblies (46, 48) positioned to optically interrogate the mixture. The excitation assembly (46) includes multiple light sources (100) and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly (48) includes multiple detectors (102) and a second set of filters for detecting light emitted from the chamber (10) in multiple emission wavelength ranges. The optics assemblies (46, 48) thus provide a multi-channel system for detcting a plurality of different target analytes in the mixture.

    Multi-channel optical detection system
    35.
    发明专利
    Multi-channel optical detection system 未知

    公开(公告)号：AU4194099A

    公开(公告)日：1999-12-06

    申请号：AU4194099

    申请日：1999-05-19

    Applicant: CEPHEID

    Inventor： CHRISTEL LEE A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A ,  YOUNG STEVEN J ,  CHANG ROBERT ,  DORITY DOUGLAS B ,  HEBERT RAYMOND ,  KINTZ GREGORY J

    IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

    Abstract: An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel (2) having a chamber (10) for holding the mixture. The apparatus also includes a heat-exchanging module (37) having a pair of opposing thermal plates (34A, 34B) for receiving the vessel (2) between them and for heating and/or cooling the mixture contained in the vessel. The module (37) also includes optical excitation and detection assemblies (46, 48) positioned to optically interrogate the mixture. The excitation assembly (46) includes multiple light sources (100) and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly (48) includes multiple detectors (102) and a second set of filters for detecting light emitted from the chamber (10) in multiple emission wavelength ranges. The optics assemblies (46, 48) thus provide a multi-channel system for detcting a plurality of different target analytes in the mixture.

    MULTI-CHANNEL OPTICAL DETECTION SYSTEM
    36.
    发明专利
    MULTI-CHANNEL OPTICAL DETECTION SYSTEM 未知

    公开(公告)号：CA2331678A1

    公开(公告)日：1999-11-25

    申请号：CA2331678

    申请日：1999-05-19

    Applicant: CEPHEID

    Inventor： KOVACS GREGORY T A ,  MCMILLAN WILLIAM A ,  YOUNG STEVEN J ,  CHANG RONALD ,  KINTZ GREGORY J ,  HEBERT RAYMOND ,  DORITY DOUGLAS B ,  PETERSEN KURT E ,  CHRISTEL LEE A ,  NORTHRUP M ALLEN

    IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

    Abstract: An apparatus for thermally controlling and optically interrogating a reactio n mixture includes a vessel (2) having a chamber (10) for holding the mixture. The apparatus also includes a heat-exchanging module (37) having a pair of opposing thermal plates (34A, 34B) for receiving the vessel (2) between them and for heating and/or cooling the mixture contained in the vessel. The modu le (37) also includes optical excitation and detection assemblies (46, 48) positioned to optically interrogate the mixture. The excitation assembly (46 ) includes multiple light sources (100) and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multip le excitation wavelength ranges. The detection assembly (48) includes multiple detectors (102) and a second set of filters for detecting light emitted from the chamber (10) in multiple emission wavelength ranges. The optics assembli es (46, 48) thus provide a multi-channel system for detcting a plurality of different target analytes in the mixture.

    Integrated fluid manipulation cartridge
    37.
    发明专利
    Integrated fluid manipulation cartridge 未知

    公开(公告)号：AU1947299A

    公开(公告)日：1999-07-19

    申请号：AU1947299

    申请日：1998-12-24

    Applicant: CEPHEID

    Inventor： POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

    IPC: G01N31/20 ,  B01D11/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

    Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.

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