中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

113 records (took 0.066 seconds)

    MICROORGANISMS FOR PRODUCING 6-AMINOCAPROIC ACID
    33.
    发明申请
    MICROORGANISMS FOR PRODUCING 6-AMINOCAPROIC ACID 审中-公开
    生产6-氨基酸的微生物

    公开(公告)号：WO2012177721A1

    公开(公告)日：2012-12-27

    申请号：PCT/US2012/043283

    申请日：2012-06-20

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony P. ,  OSTERHOUT, Robin E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P7/04 ,  C12N1/21

    CPC classification number: C12N15/52 ,  C12P13/005

    Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, a 6-aminocaproic acid, caprolactam, or hexametheylenediamine pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine pathway and at least one exogenous nucleic acid that encodes an enzyme that increases the yields of adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine by (i) enhancing carbon fixation via the reductive TCA cycle, and/or (ii) accessing additional reducing equivalents from gaseous carbon sources and/or syngas components such as CO, C02, and/or H2. The invention additionally provides a method for producing adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine. The method can include culturing an adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism described herein under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid, caprolactam or hexametheylenediamine.

    Abstract translation: 本发明提供了具有己二酸酯，6-氨基己酸，己内酰胺或六甲苯二胺途径的非天然存在的微生物。 微生物生物体含有至少一种编码相应的己二酸，6-氨基己酸，己内酰胺或六甲苯二胺途径中的酶的外源核酸，以及至少一种外源核酸，其编码增加己二酸，6-氨基己酸， （i）通过还原TCA循环增强碳固定，和/或（ii）从气态碳源和/或合成气组分如CO，CO 2和/或H 2获得额外的还原当量。 本发明另外提供了生产己二酸，6-氨基己酸，己内酰胺或六亚甲基二胺的方法。 该方法可以包括在条件和足够的时间内培养己二酸酯，6-氨基己酸，己内酰胺或六亚甲基二胺产生的微生物生物体以产生己二酸，6-氨基己酸，己内酰胺或六亚甲基二胺。

    MICROORGANISMS FOR PRODUCING ISOBUTANOL AND METHODS RELATED THERETO
    34.
    发明申请
    MICROORGANISMS FOR PRODUCING ISOBUTANOL AND METHODS RELATED THERETO 审中-公开
    用于生产ISOBUTANOL的微生物及其相关方法

    公开(公告)号：WO2012177601A2

    公开(公告)日：2012-12-27

    申请号：PCT/US2012/043091

    申请日：2012-06-19

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P7/16

    CPC classification number: C12P7/16 ,  C12N15/52 ,  Y02E50/10

    Abstract: Provided herein is a non-naturally occurring microbial organism having an isopropanol pathway and comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. In certain embodiments, the pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. Also provided herein is anon-naturally occurring microbial organism having an n -butanol pathway and comprising at least one exogenous nucleic acid encoding an n -butanol pathway enzyme. Other non-naturally occurring microbial organism having n -butanol or isobutanol pathways are also provided herein. In certain embodiments, isobutanol pathways utilizing reverse TCA and/or reducing equivalents from CO and/or hydrogen are used to enhance product yields. The organisms provided herein can be cultured to produce isopropanol, n -butanol, or isobutanol.

    Abstract translation: 本文提供了具有异丙醇途径并且包含至少一种编码异丙醇途径酶的外源核酸的非天然存在的微生物生物。 在某些实施方案中，该途径包括选自4-羟基丁酰-CoA脱水酶，巴豆酸酶，3-羟基丁酰辅酶A脱氢酶，乙酰乙酰辅酶A合成酶，乙酰辅酶A：乙酰乙酸酯-CoA转移酶，乙酰乙酰辅酶A 水解酶，乙酰乙酸脱羧酶和丙酮还原酶。 本文还提供了具有正丁醇途径的非天然存在的微生物生物，并且包含至少一种编码正丁醇途径酶的外源核酸。 本文还提供了其它具有正丁醇或异丁醇途径的非天然存在的微生物。 在某些实施方案中，使用利用反向TCA和/或来自CO和/或氢的还原当量的异丁醇途径来提高产物产率。 本文提供的生物体可以培养以产生异丙醇，正丁醇或异丁醇。

    MICROORGANISMS FOR PRODUCING N-PROPANOL 1, 3-PROPANEDIOL, 1,2-PROPANEDIOL OR GLYCEROL AND METHODS RELATED THERETO
    35.
    发明申请
    MICROORGANISMS FOR PRODUCING N-PROPANOL 1, 3-PROPANEDIOL, 1,2-PROPANEDIOL OR GLYCEROL AND METHODS RELATED THERETO 审中-公开
    用于生产N-丙醇1,3-丙二醇，1,2-丙二醇或甘油的微生物及其相关方法

    公开(公告)号：WO2012177599A2

    公开(公告)日：2012-12-27

    申请号：PCT/US2012/043086

    申请日：2012-06-19

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P7/04 ,  C12P7/20

    CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12N9/0067 ,  C12P7/04 ,  C12P7/20

    Abstract: Provided herein is a non-naturally occurring microbial organism having a reductive TCA or Wood-Ljungdahl pathway and comprising at least one exogenous nucleic acid encoding said pathway enzymes expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. Also provided herein is a method for enhancing carbon flux through acetyl-CoA and in some embodiments includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism provided herein includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. Also provided herein is a method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, and can include culturing this organism for a sufficient period of time to produce a product.

    Abstract translation: 本文提供了具有还原性TCA或Wood-Ljungdahl途径的非天然存在的微生物生物体，并且包含至少一种编码所述途径酶的外源核酸，其以足够的量表达以增强通过乙酰辅酶A的碳通量。 本文还提供了通过乙酰辅酶A增强碳通量的方法，并且在一些实施方案中包括在条件和足够的时间段内培养这些非天然存在的微生物生物体以产生具有乙酰辅酶A作为结构单元的产物。 本文提供的另一种非天然存在的微生物包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过经由 碳水化合物碳原料。 本文还提供了在一氧化碳或氢气存在下增强还原当量的可用性的方法，并且可以包括在足够的时间内培养该生物体以产生产物。

    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL
    36.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 审中-公开
    用于生产1,3-丁二醇的有机物

    公开(公告)号：WO2010127319A2

    公开(公告)日：2010-11-04

    申请号：PCT/US2010/033300

    申请日：2010-04-30

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  PHARKYA, Priti

    IPC: C12N1/21 ,  C12P7/18

    CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3- BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4- hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA- dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    Abstract translation: 非天然存在的微生物有机体包括具有1,3-丁二醇（1,3-BDO）途径的微生物，其具有至少一种编码1,3-BDO途径酶的外源核酸 以足以产生1,3-BDO的量表达。 该途径包括选自2-氨基-4-酮戊酸（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸氨基转移酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2-氨基-4-羟基戊酸 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨基），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（酮还原），3-氧代丁醛还原酶（醛还原 ），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮转氨酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶 ，丁烯酮水合酶，AKP氨 - 裂解酶，乙酰基丙烯酸酯脱羧酶，乙酰乙酰-CoA还原酶（CoA依赖性，醛形成），乙酰乙酰-CoA还原酶（CoA依赖性，形成醇），乙酰乙酰-CoA还原酶（酮 还原），3-羟基丁酰-CoA还原酶（醛形成），3-羟基 乌乙酰-CoA还原酶（形成醇），4-羟基丁酰-CoA脱水酶和巴豆酸酶。 生产1,3-BDO的方法包括在一定条件下培养这种微生物并持续足够的时间以产生1,3-BDO。

    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL
    37.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL 审中-公开
    用于生产甲基乙基酮和2-丁醇的微生物

    公开(公告)号：WO2010057022A1

    公开(公告)日：2010-05-20

    申请号：PCT/US2009/064433

    申请日：2009-11-13

    Applicant: GENOMATICA, INC. ,  BURK, Mark, J. ,  PHARKYA, Priti ,  BURGARD, Anthony, P.

    Inventor： BURK, Mark, J. ,  PHARKYA, Priti ,  BURGARD, Anthony, P.

    IPC: C12P7/26 ,  C12P7/00 ,  C12N1/21

    CPC classification number: C12P7/16 ,  C12N15/52 ,  C12P7/26 ,  Y02E50/10

    Abstract: A non-naturally occurring microbial organism having a methyl ethyl ketone pathway includes at least one exogenous nucleic acid encoding a methyl ethyl ketone pathway enzyme expressed in a sufficient amount to produce methyl ethyl ketone. The methyl ethyl ketone pathway includes a β-ketothiolase, a β-ketovalerate decarboxylase and an enzyme selected from the group consisting of a β-ketovaleryl-CoA hydrolase and a β-ketovaleryl-CoA transferase. Alternatively, the methyl ethyl ketone pathway includes a 2-methylacetoacetyl-CoA thiolase, a 2-methylacetoacetate decarboxylase and an enzyme selected from the group consisting of a 2-methylacetoacetyl-CoA hydrolase and a 2-methylacetoacetyl-CoA transferase. Either pathway can further include a methyl ethyl ketone reductase to produce 2-BuOH. A method for producing methyl ethyl ketone or 2-BuOH includes culturing these non-naturally occurring microbial organisms under conditions, and for a sufficient period of time, to produce methyl ethyl ketone or 2-BuOH.

    Abstract translation: 具有甲基乙基酮途径的非天然存在的微生物包括至少一种编码甲基乙基酮途径酶的外源核酸，其以足以产生甲基乙基酮的量表达。 甲基乙基酮途径包括β-酮硫解酶，β-酮戊二酸脱羧酶和选自β-酮戊酰基-CoA水解酶和β-酮戊酰基-CoA转移酶的酶。 或者，甲基乙基酮途径包括2-甲基乙酰乙酰辅酶A硫解酶，2-甲基乙酰乙酸脱羧酶和选自2-甲基乙酰乙酰辅酶A水解酶和2-甲基乙酰乙酰辅酶A转移酶的酶。 任一途径还可以包括甲基乙基酮还原酶以产生2-BuOH。 一种生产甲基乙基酮或2-BuOH的方法包括在条件和足够的时间内培养这些非天然存在的微生物生产甲基乙基酮或2-BuOH。

Patent Agency Ranking