公开(公告)号：KR1020110090518A

公开(公告)日：2011-08-10

申请号：KR1020100010346

申请日：2010-02-04

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김재조 ,  송재영 ,  임성인 ,  조인수 ,  김병한 ,  한규하 ,  현방훈

IPC: C12N15/65 ,  A61K39/12

Abstract: PURPOSE: A live vaccine containing a recombinant chimeric pestivirus for preventing swine fever is provided to easily trace virus for the live vaccine. CONSTITUTION: A live vaccine for preventing swine fever contains chimeric pestivirus KD26_E2LOM of sequence number 2 which is recombined to discriminate vaccine virus and outdoor-infectious virus. A gene marker is expressed by substituting E2 gene of pACBVD26 of sequence number 1 with a swine fever E2 gene. A serological marker is a marker for E^rns specific antibody.

Abstract translation: 目的：提供含有用于预防猪瘟的重组嵌合瘟病毒的活疫苗，以便轻松追踪活疫苗的病毒。 构成：用于预防猪瘟的活疫苗包含序列号为2的嵌合瘟病毒KD26_E2LOM，其重组以区分疫苗病毒和室外感染性病毒。 通过用猪瘟E2基因取代序列号1的pACBVD26的E2基因来表达基因标记。 血清学标记是E ^ rns特异性抗体的标记。

公开(公告)号：KR100834014B1

公开(公告)日：2008-06-02

申请号：KR1020060102854

申请日：2006-10-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김연희 ,  탁동섭 ,  임성인 ,  현방훈 ,  김인중 ,  송재영 ,  양동군 ,  권창희 ,  권준헌

IPC: C12N7/01 ,  A61K39/12

Abstract: 본 발명은 모기를 매개로 소에서 유사산을 일으키는 소 아까바네병, 츄잔병 및 아이노 바이러스 감염증 예방을 위한 아까바네(K-9), 츄잔(YoungAM) 및 아이노(KSA9910) 바이러스 균주를 이용한 3종 혼합 바이러스 백신 및 그 제조방법에 관한 것으로서, 보다 상세하게는 국내 분리주인 아까바네(K-9), 츄잔(YoungAM) 및 아이노(KSA9910) 바이러스 균주를 불활화시킨 후 이들을 면역증강제와 함께 혼합하여 제조함으로써 3가지 바이러스를 한가지 예방약으로 예방이 가능하도록 제조한 3종 혼합 바이러스 백신 및 그 제조방법에 관한 것이다.
소 아까바네병, 아이노바이러스 감염증, 츄잔병, 3종 혼합불활화 백신

公开(公告)号：KR1020080036353A

公开(公告)日：2008-04-28

申请号：KR1020060102854

申请日：2006-10-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 김연희 ,  탁동섭 ,  임성인 ,  현방훈 ,  김인중 ,  송재영 ,  양동군 ,  권창희 ,  권준헌

IPC: C12N7/01 ,  A61K39/12

Abstract: A viral vaccine containing isolated virus strains is provided to improve use safety and immunogenicity by using inactivated viruses, and increase production yield of cows, so that the vaccine is useful for prevention of abortion or stillbirth in cows caused by viruses via mediation of Culicoides spp. A viral vaccine for prevention of abortion or stillbirth in cows contains cultured medium of inactivated virus strains of akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P), and is produced by: inactivating akabane virus(K-9)(KCTC 18117P), chuzan virus(KSA9910)(KCTC 18118P) and aino virus(YoungAM)(KCTC 18119P) by adding 0.1M BEI(binary ethylene imine) prepared by solubilizing 2-BEA(2-bromoethylamine) in 0.2N NaOH, and treating the solution in a constant temperature water bath of 37 deg. C for 1 hour to them; mixing the 3 kinds of viruses in a weight ratio of 1:1:1; and adding 40-60 wt.% of IMS 1314 as an immunomodulator into the viral mixture.

Abstract translation: 提供含有分离的病毒株的病毒疫苗，以通过使用灭活病毒提高使用安全性和免疫原性，并提高奶牛的产量，使得疫苗可用于通过Culicoides spp的调解来预防由病毒引起的奶牛的流产或死胎。 用于预防奶牛流产或死胎的病毒疫苗含有阿卡巴因病毒（K-9）（KCTC18117P），Chuzan病毒（KSA9910）（KCTC18118P）和Aino病毒（YoungCAM）（KCTC18119P）的灭活病毒株的培养基， 通过添加由0.1M BEI（二元乙烯亚胺）制备的阿卡巴因病毒（K-9）（KCTC 18117P），Chuzan病毒（KSA9910）（KCTC 18118P）和Aino病毒（YoungAM）（KCTC 18119P） 溶解在0.2N NaOH中的2-BEA（2-溴乙胺），并在37℃恒温水浴中处理该溶液。 C给他们1小时; 混合3种病毒，重量比为1：1：1; 并将40-60重量％的IMS 1314作为免疫调节剂加入到病毒混合物中。

公开(公告)号：KR1020080010771A

公开(公告)日：2008-01-31

申请号：KR1020060071258

申请日：2006-07-28

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 권창희 ,  양동군 ,  임성인 ,  탁동섭 ,  권준헌

IPC: C12N15/12

Abstract: Recombinant proteins of enzootic bovine leukemia virus are provided to improve specificity and sensitivity of detection as compared with conventional antigens by expressing the envelope glycoproteins in baculovirus and secreting the envelope glycoproteins to insect cells. A method for producing a recombinant protein of enzootic bovine leukemia virus expressed in an insect cell comprises the steps of: (1) cloning an envelop glycoprotein of enzootic bovine leukemia virus from the blood of enzootic bovine leukemia positive cows to baculovirus and sequencing the cloned protein; (2) transforming an insect cell with a recombinant virus gene of SEQ ID NO:1 to express a recombinant protein of SEQ ID NO:2; and (3) purifying and concentrating the recombinant protein produced from the insect cell, wherein the recombinant virus gene of SEQ ID NO:1 is prepared by recombination of envelop glycoprotein genes including a whole gp51 gene and a part of gp30 gene.

Abstract translation: 通过在杆状病毒中表达包膜糖蛋白并将包膜糖蛋白分泌到昆虫细胞中，提供了特异性和灵敏度的检测与常规抗原的特异性和灵敏度。 用于生产在昆虫细胞中表达的特异性牛白血病病毒重组蛋白质的方法包括以下步骤：（1）将来自地方性牛白血病阳性牛的血液中的特异性牛白血病病毒的包膜糖蛋白克隆至杆状病毒，并测序克隆的蛋白质 ; （2）用SEQ ID NO：1的重组病毒基因转化昆虫细胞以表达SEQ ID NO：2的重组蛋白; （3）从昆虫细胞产生的重组蛋白质的纯化和浓缩，其中通过包括整个gp51基因和一部分gp30基因的包膜糖蛋白基因的重组制备SEQ ID NO：1的重组病毒基因。