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42.发明授权바이셀라 코리엔시스의 특이적 프라이머 쌍 및 이를 이용한 바이셀라 코리엔시스의 검출방법 有权一种特定的芽孢杆菌（Bacillaria）的引物对和一种用于检测百里香（Bacela corinisis）的方法
公开(公告)号：KR101750850B1
公开(公告)日：2017-06-29
申请号：KR1020150126864
申请日：2015-09-08
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은김치유산균인바이셀라코리엔시스검출용프라이머쌍 및이을포함하는바이셀라코리엔시스의검출방법에관한것이다. 본발명인서열번호 1 및서열번호 2의염기서열로표시되는김치유산균바이셀라코리엔시스검출용프라이머쌍 및이를포함하는 PCR을이용한검출방법은김치발효중에존재하는바이셀라코리엔시스의존재여부를시기별로정확하고신속하게확인함으로써균주확인에따른시간과비용을절감시킬수 있다. 또한, 김치발효동안에바이셀라코리엔시스균주를시기별로정량분석하여김치의맛과풍미를결정하는바이셀라코리엔시스의활성정도를표준화시켜김치품질조절을위한도구로사용할수 있다.
Abstract translation: 本发明涉及由含有由科里地下室的N- Sys系统检测引物对和用于秋泡菜乳酸菌科里的N- SYS将窖的检测方法。 本发明SEQ ID NO：1和SEQ ID NO：使用两对用于通过地窖科里测序泡菜乳酸菌引物检测方法表示由N个-Sys系统检测和PCR含有相同被不时通过-地下室科里的N- Sys系统存在于所述泡菜发酵存在 准确快速的识别可以节省应变识别的时间和成本。 此外，标准化可以用作质量控制酸菜的工具的副酒窖科里的N- Sys系统通过泡菜发酵期间地窖科里的N- Sys系统应变确定从时间泡菜和定量分析的口味和风味，以时间的活性量。
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公开(公告)号：KR1020170029810A
公开(公告)日：2017-03-16
申请号：KR1020150126864
申请日：2015-09-08
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은김치유산균인바이셀라코리엔시스검출용프라이머쌍 및이을포함하는바이셀라코리엔시스의검출방법에관한것이다. 본발명인서열번호 1 및서열번호 2의염기서열로표시되는김치유산균바이셀라코리엔시스검출용프라이머쌍 및이를포함하는 PCR을이용한검출방법은김치발효중에존재하는바이셀라코리엔시스의존재여부를시기별로정확하고신속하게확인함으로써균주확인에따른시간과비용을절감시킬수 있다. 또한, 김치발효동안에바이셀라코리엔시스균주를시기별로정량분석하여김치의맛과풍미를결정하는바이셀라코리엔시스의활성정도를표준화시켜김치품질조절을위한도구로사용할수 있다.
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44.发明公开
公开(公告)号：KR1020140064499A
公开(公告)日：2014-05-28
申请号：KR1020120131899
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장) , 경기대학교 산학협력단
CPC classification number: C12Q1/6851 , C12N15/11 , C12Q1/686
Abstract: The present invention relates to a a polymerase chain reaction (PCR) primer for detecting pectobacterium carotovorum subsp. carotovorum and a method for detecting the pectobacterium carotovorum subsp. carotovorum using the same. A user can detect the pectobacterium carotovorum subsp. carotovorum immediately if the user uses the PCR primer according to the present invention an follows the conditions of gene amplification.
Abstract translation: 本发明涉及一种用于检测carotovorum子孢子菌的聚合酶链反应（PCR）引物。 carotovorum和一种检测carotovorum子茎的方法。 carotovorum使用相同。 用户可以检测到胡萝卜素（carotovorum subsp。） 如果用户使用根据本发明的PCR引物的carotovorum，则遵循基因扩增的条件。
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公开(公告)号：KR101359531B1
公开(公告)日：2014-02-13
申请号：KR1020110043519
申请日：2011-05-09
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 고추냉이 연부병(Soft rot)의 원인균인 펙토박테리움 와사비에(
Pectobacterium
wasabiae
) 검출용 프라이머 세트, 이를 이용한 펙토박테리움 와사비에의 검출방법, 이를 포함하는 펙토박테리움 와사비에 검출용 조성물 및 PCR 키트에 관한 것이다.
본 발명에 따른 프라이머 세트를 이용하면 고추냉이 연부병의 원인균인 펙토박테리움 와사비에를 신속하고 정확하게 검출할 수 있어 고추냉이 연부병의 감염 여부를 효과적으로 진단할 수 있으므로, 고추냉이 연부병을 예방하고 방제하는데 기여할 수 있다.-
Patent Agency Ranking
公开(公告)号：KR1020120125835A
公开(公告)日：2012-11-19
申请号：KR1020110043519
申请日：2011-05-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6844 , C12Q1/04 , C12R1/01
Abstract: PURPOSE: A primer set for detecting Pectobacterium wasabiae causing soft rot is provided to quickly and accurately detect Pectobacterium wasabiae and to prevent soft rot. CONSTITUTION: A primer set for detecting Pectobacterium wasabiae contains a primer with nucleic acid sequences of sequence numbers 1 and 2. A method for detecting Pectobacterium wasabiae comprises a step of amplifying DNA isolated from a plant sample and a step of identifying the PCR products. A composition for detecting Pectobacterium wasabiae contains the primer set. A PCR kit contains the primer set.
Abstract translation: 目的：提供一种用于检测引起软腐病的棘皮杆菌的引物组，用于快速准确地检测败血性贝氏杆菌并防止软腐。 构成：用于检测棘皮杆菌的引物组含有具有序列号1和2的核酸序列的引物。检测棘皮杆菌的方法包括扩增从植物样品分离的DNA的步骤和鉴定PCR产物的步骤。 用于检测棘皮杆菌的组合物含有引物组。 PCR试剂盒包含引物组。
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公开(公告)号：KR1020090125572A
公开(公告)日：2009-12-07
申请号：KR1020080051740
申请日：2008-06-02
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: A method for selecting a gene from a microarray is provided to easily detect a novel gene which is not classified through a conventional method. CONSTITUTION: A method for screening a candidate gene through microarray data analysis comprises: a step of measuring expression intensity from each spot of microarray; a step of calculating expression intensity rate and standard value through maximum boundary from value data(absolute value of the expression intensity); and a step of comparing absolute value, and standard value to expression intensity rate to select candidate gene. An analysis apparatus of nucleic acid or protein microarray comprises: a detection unit which measures expression intensity from each spot of microarray; a calculation unit which calculates expression intensity rate and standard value; a database which storing expression intensity rate and standard value; a selection part which chooses candidate genes; and an output unit which outputs value data, selected candidate gene, expression intensity rate and standard value.
Abstract translation: 目的：提供从微阵列中选择基因的方法，以容易地检测未通过常规方法分类的新基因。 构成：通过微阵列数据分析筛选候选基因的方法包括：从微阵列的每个点测量表达强度的步骤; 从值数据（表达强度的绝对值）通过最大边界计算表达强度率和标准值的步骤; 并将绝对值和标准值与表达强度比值进行比较，以选择候选基因。 核酸或蛋白质微阵列的分析装置包括：检测单元，其测量每个微阵列点的表达强度; 计算表达强度率和标准值的计算单元; 存储表达强度率和标准值的数据库; 选择候选基因的选择部分; 以及输出值数据，选择候选基因，表达强度率和标准值的输出单元。
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48.发明公开풋마름병원균 특이 디엔에이 단편 검출을 위한 합성올리고뉴클레오티드 및 이들을 이용한 풋마름병 병원균에감염된 식물체의 검출 방법 有权用于敏感和特异性检测RALSTONIA SOLANACEARUM的合成寡核苷酸和使用它们检测由RALSTONIA SOLANACEARUM感染的植物的方法
公开(公告)号：KR1020080095394A
公开(公告)日：2008-10-29
申请号：KR1020070039792
申请日：2007-04-24
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6844 , C12Q1/6888 , C12Q2565/125
Abstract: A synthesis oligonucleotide is provided to diagnose quickly and conveniently whether being infected with Ralstonia solanacearum or not by performing the PCR amplification and to detect the Ralstonia solanacearum specific DNA fragment. A synthesis oligonucleotide has base sequences of a sequence number 2 and a sequence number 3. The synthesis oligonucleotide Is specific to No. 1~1,341 bp or 21~952 bp of the cytochrome c1 signal peptide of the Ralstonia solanacearum which is the Ralstonia solanacearum having the base sequence 1.
Abstract translation: 提供合成寡核苷酸以通过进行PCR扩增和检测茄属Ralstonia solanacearum特异性DNA片段快速方便地诊断是否感染Ralstonia solanacearum。 合成寡核苷酸具有序列号2和序列号3的碱基序列。合成寡核苷酸特异性为茄属Ralstonia solanacearum的Ralstonia solanacearum的1〜1341bp或21〜952bp的细胞色素c1信号肽，其具有 基本序列1。
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50.发明公开십자화과 흑부병원균의 특이적 검출을 위한 프라이머 세트및 이를 이용한 흑부병원균의 검출방법 有权用于特异性鉴定病原体X光体的初步设置。 CAMPESTRIS和使用该方法的病原体的特异性鉴定方法
公开(公告)号：KR1020040079052A
公开(公告)日：2004-09-14
申请号：KR1020030013954
申请日：2003-03-06
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Primer sets for specific identification of a pathogen, Xanthomonas campestris pv. campestris, and a specific-identification method of the pathogen using the same primers are provided to rapidly and accurately identify Xanthomonas campestris pv. campestris. Therefore, the primer sets can be useful for prevention and control of Xanthomonas campestris pv. campestris. CONSTITUTION: The primer sets for specific identification of a pathogen, Xanthomonas campestris pv. campestris, amplify the total or a portion of 1st to 650th nucleotides in the nucleotide sequence of SEQ ID NO:1 of a gene hrpF, wherein the primer sets have the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3. The specific-identification method of Xanthomonas campestris pv. campestris comprises the steps of: (A) extracting the total DNA from a plant sample; (B) PCR amplifying the extracted total DNA; and (C) verifying the amplification of the total or a portion of 1st to 650th nucleotides in the nucleotide sequence of SEQ ID NO:1 of a gene hrpF.
Abstract translation: 目的：用于特异性鉴定病原体的引物组，野油菜黄单胞菌（Xanthomonas campestris pv。） 提供了使用相同引物的病原体的特异性鉴定方法，以快速准确地鉴定野油菜黄单胞菌（Xanthomonas campestris pv。 油菜。 因此，引物组可用于预防和控制野油菜黄单胞菌（Xanthomonas campestris pv。 油菜。 构成：用于特异性鉴定病原体的引物组，野油菜黄单胞菌（Xanthomonas campestris pv。 菜籽油，扩增基因hrpF的SEQ ID NO：1的核苷酸序列中第1至第650位核苷酸的总数或部分，其中引物组具有SEQ ID NO：2和SEQ ID NO：3的核苷酸序列。 野油菜黄单胞菌的特异性鉴定方法 营养食品包括以下步骤：（A）从植物样品中提取总DNA; （B）PCR扩增提取的总DNA; 和（C）验证基因hrpF的SEQ ID NO：1的核苷酸序列中第1至第650位核苷酸的总数或部分的扩增。
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