公开(公告)号：KR1020090022698A

公开(公告)日：2009-03-04

申请号：KR1020070088280

申请日：2007-08-31

Applicant: 삼성전자주식회사

Inventor： 김원선 ,  하정환 ,  류만형 ,  지성민 ,  김형준 ,  정선옥

IPC: C12Q1/68

CPC classification number: B01L9/527 ,  B01L3/5027 ,  B01L2300/16

Abstract: A method for coating a silane layer on a substrate is provided to prevent misalignment between a substrate and other components in a manufacturing process of biochips. A method for coating a silane layer on a substrate comprises the following steps of: preparing the substrate(10); spin-coating the substrate with silane(300); and coupling a probe on a silane layer. The spin-coating includes the following steps of: coating a silane solution on the substrate; rotating the silane solution-coated substrate at a first speed and rotating again at a second speed; and solidifying the silane solution. The first speed is 500rpm, and the second speed is 2,000rpm.

Abstract translation: 提供了在基板上涂布硅烷层的方法，以防止在生物芯片的制造过程中基板与其它部件之间的错位。 在衬底上涂覆硅烷层的方法包括以下步骤：制备衬底（10）; 用硅烷（300）旋涂底物; 并将探针偶联在硅烷层上。 旋涂包括以下步骤：将硅烷溶液涂布在基材上; 以第一速度旋转硅烷溶液涂覆的基底并以第二速度再次旋转; 并固化硅烷溶液。 第一速度为500rpm，第二速度为2000rpm。

公开(公告)号：KR1020090021636A

公开(公告)日：2009-03-04

申请号：KR1020070086289

申请日：2007-08-27

Applicant: 삼성전자주식회사

Inventor： 김원선 ,  하정환 ,  지성민 ,  정선옥 ,  류만형

IPC: G01N33/53 ,  G01N33/48

CPC classification number: G01N33/54386 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00443 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B82Y30/00

Abstract: A biochip for analyzing components of a biosample is provided to improve analysis reliability by preventing data noises and restraining an undesired coupling phenomenon of linkers or probes on a rear surface of a substrate. A biochip(11) comprises a substrate (100), a capping layer pattern(110), and a plurality of probes(140). The capping layer pattern partly covers a front surface of the substrate. The plurality of probes are coupled on the substrate exposed by the capping layer pattern. The substrate includes a plurality of probe cell areas(I) and non-probe cell areas(II). The non-probe cell areas separate the each probe cell area. The capping layer pattern is located on the non-probe cell area.

Abstract translation: 提供用于分析生物样品组分的生物芯片，以通过防止数据噪声并抑制衬底背面上的接头或探针的不期望的耦合现象来提高分析可靠性。 生物芯片（11）包括衬底（100），覆盖层图案（110）和多个探针（140）。 封盖层图案部分地覆盖基板的前表面。 多个探针耦合在由覆盖层图案暴露的基板上。 基板包括多个探针单元区域（I）和非探针单元区域（II）。 非探针单元区域分离每个探针单元区域。 覆盖层图案位于非探针单元区域上。

公开(公告)号：KR100834745B1

公开(公告)日：2008-06-09

申请号：KR1020060131208

申请日：2006-12-20

Applicant: 삼성전자주식회사

Inventor： 하정환 ,  지성민 ,  김경선 ,  김원선 ,  조한구

IPC: C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00693 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6837 ,  Y10T428/24612 ,  B01J2219/00603 ,  C12Q2565/513

Abstract: An oligomer probe array chip is provided to be based on an analysis-friendly layout compatible with a CCD(charge coupled device) scanner operated by a scan-by area manner. An oligomer probe array chip comprises: a substrate; a main array arranged on the substrate including a plurality of stripe-type sub-arrays, each of the sub-arrays arranged in a matrix form and consisting of a plurality of spots where an oligomer probe with a sequence different from each other is fixed; and a plurality of sub-array align spot arrays, each of which is arranged on the outside of the each sub-arrays respectively. A mask(100) for forming an oligomer probe array chip comprises: a main array pattern(110) including a plurality of stripe-type sub-array patterns(120), each of the sub-array patterns consisting of a plurality of spot patterns(135,155) arranged in a matrix form; and a plurality of spot array patterns(130), each of which is arranged on the outside of the each sub-array patterns respectively. A method for hybridization analysis of the oligomer probe array chip comprises the steps of: (a) hybridizing a target sample into the oligomer array chip; (b) determining a position of the stripe-type sub-array using the sub-array align spot array; (c) forming an image of the stripe-type sub-array using a CCD scanner; (d) repeating the steps(a), (b), and (c) on all the stripe-type sub-arrays to generate all stripe-type sub-array images; and (e) aligning the sub-array images to generate one hybridized image. Further, a global-array align spot array separated from the sub-array align spot array is additionally contained outside the main array.

Abstract translation: 基于与通过扫描区域方式操作的CCD（电荷耦合器件）扫描兼容的分析友好的布局来提供低聚物探针阵列芯片。 低聚物探针阵列芯片包括：基底; 布置在基板上的主阵列，包括多个条形子阵列，每个子阵列以矩阵形式排列并且由多个点组成，其中具有彼此不同的序列的低聚物探针是固定的; 以及分别配置在每个子阵列的外侧的多个子阵列对准光点阵列。 用于形成低聚物探针阵列芯片的掩模（100）包括：主阵列图案（110），包括多个条形子阵列图案（120），每个子阵列图案由多个斑点图案 （135,155）以矩阵形式排列; 以及多个点阵列图案（130），其分别布置在每个子阵列图案的外侧。 寡聚体探针阵列芯片的杂交分析方法包括以下步骤：（a）将目标样品与低聚物阵列芯片杂交; （b）使用子阵列对准点阵列确定条形子阵列的位置; （c）使用CCD扫描仪形成条形子阵列的图像; （d）在所有条形型子阵列上重复步骤（a），（b）和（c）以产生所有条形型子阵列图像; 和（e）对准子阵列图像以产生一个杂交图像。 此外，与子阵列对准光点阵列分离的全局阵列对准光点阵列另外包含在主阵列之外。

公开(公告)号：KR1020080015338A

公开(公告)日：2008-02-19

申请号：KR1020060076899

申请日：2006-08-14

Applicant: 삼성전자주식회사

Inventor： 지성민 ,  하정환 ,  김경선 ,  김원선 ,  최상준 ,  류만형

IPC: C07C205/34 ,  C07H19/00 ,  C07C205/42

Abstract: A photo-labile compound is provided to increase a photolysis reaction rate, and thus improve total reaction yield when applied to a manufacture process of oligomer probe array. A photo-labile compound has a structure represented by the following formula 1, wherein R1 is hydrogen, an alkyl group having 1-3 carbon atoms, or an alkoxy group having 1-3 carbon atoms, X is the formula (a) or (b), and Y is the following formula (Y).

Abstract translation: 提供光不稳定化合物以提高光解反应速率，从而在应用于低聚物探针阵列的制备方法时提高总反应产率。 光稳定化合物具有下式1所示的结构，其中R 1为氢，具有1-3个碳原子的烷基或具有1-3个碳原子的烷氧基，X为式（a）或（ b），Y为下式（Y）。

公开(公告)号：KR100772893B1

公开(公告)日：2007-11-05

申请号：KR1020060039713

申请日：2006-05-02

Applicant: 삼성전자주식회사

Inventor： 하정환 ,  지성민 ,  김경선 ,  김원선

IPC: G01N33/53 ,  G01N33/50 ,  G01N33/48

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/00662 ,  B01J2219/00722

Abstract: An oligomer probe array in which an oligomer probe is coupled only to a cell active region and not coupled to a probe cell separation region surrounding the probe cell active region is provided to increase the signal to noise ratio and assay intensity. An oligomer probe array comprises a substrate(100); a probe cell separation region(130) which does not include a functional group(150) coupled to an oligomer probe on the surface; and a plurality of probe cell active regions(120) which is separated by the probe cell separation region, formed on or in the substrate, and forms at least one groove formed on the surface of each of the probe cell active region; and a plurality of oligomer probes(160) which is coupled by each of the probe cell active region and has a sequence different from one another. The surface of the probe cell active region includes a functional group coupled to the oligomer probe, wherein a portion of the functional group is coupled to the oligomer probe and the other functional group is inactively capped. The probe cell active region is a pattern of a film formed on the substrate, an LOCOS(local oxidation silicon) film formed by topically oxidizing the substrate or a trench-type active, and the oligomer probe is coupled to the probe cell active region via a linker(140).

Abstract translation: 提供寡聚体探针阵列，其中寡聚体探针仅耦合到细胞活性区并且不耦合到围绕探针细胞活性区的探针细胞分离区，以增加信噪比和测定强度。 低聚物探针阵列包括基底（100）; 不包括与表面上的低聚物探针耦合的官能团（150）的探针细胞分离区（130） 以及形成在所述基板上或所述基板上的，由所述探针单元分离区隔开的多个探针单元活性区域，形成在所述探针单元有源区域的每一个的表面上形成的至少一个沟槽; 和多个寡聚体探针（160），其通过探针单元有源区域中的每一个耦合并且具有彼此不同的序列。 探针单元活性区域的表面包括与低聚物探针偶联的官能团，其中官能团的一部分与低聚物探针偶联，另一个官能团被非封闭。 探针单元有源区是在基板上形成的膜的图案，通过局部氧化基板或沟槽型活性物而形成的LOCOS（局部氧化硅）膜，并且低聚物探针经由探针单元有源区经由 一个接头（140）。