公开(公告)号：KR101718681B1

公开(公告)日：2017-03-22

申请号：KR1020140076720

申请日：2014-06-23

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  최윤희

IPC: C12N9/10 ,  C12N15/54 ,  C12N15/09 ,  C12P19/18 ,  G01N31/22

CPC classification number: C12N9/1051 ,  C12P19/00 ,  C12P19/18 ,  C12Q1/48 ,  C12Y204/01065 ,  G01N2333/91102

Abstract: 본발명은가용성단백질발현량및 활성이증대된α-1,3 푸코실전달효소변이체, α-1,3 푸코실전달효소변이체를코딩하는 DNA, α-1,3 푸코실전달효소변이체를코딩하는 DNA를포함하는재조합 DNA 벡터, 상기재조합 DNA 벡터로형질전환된숙주세포및 숙주세포의추출물, 3-푸코실올리고당의제조방법, 푸코실전달효소변이체제조방법및 푸코실전달효소의변이체탐색방법을제공하는것으로서, 본발명의α-1,3 푸코실전달효소변이체는가용성단백질의발현량및 효소활성이획기적으로향상된효과가있다.

公开(公告)号：KR101648342B1

公开(公告)日：2016-08-16

申请号：KR1020140076718

申请日：2014-06-23

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  최윤희

IPC: C12N15/54 ,  C12N15/09 ,  C12N9/12 ,  C12P19/18

CPC classification number: C12N9/12 ,  C12N15/09 ,  C12P19/18

Abstract: 본발명은헬리코박터파일로리 26695() 유래의α-1,2 푸코실전달효소 (α-1,2 fucosyltransferase)의난발현성재조합단백질에대한가용성단백질발현을증가시키는방법과그로인해생성된α-1,2 푸코실전달효소를코딩하는유전자, 상기유전자의발현방법, 단백질및 이효소를이용한α-1,2 푸코실올리고당의합성에관한것이다.

公开(公告)号：KR101574952B1

公开(公告)日：2015-12-07

申请号：KR1020130039938

申请日：2013-04-11

Applicant: 서울대학교산학협력단 ,  주식회사 진켐

Inventor： 김병기 ,  최윤희 ,  김종훈 ,  김대희 ,  우진석

IPC: C12Q1/48 ,  C12N15/52 ,  C12P19/04

Abstract: 본발명은시알산전이효소변이체의탐색방법과변이체의특성, 상기시알산전이효소변이체효소를암호화하는유전자및 이들을이용한시알릴올리고당의합성에관한것이다. 본발명은단백질공학적변이를통해개량된파스테우렐라유래의α-2,3 시알산전이효소와포토박테리움유래의α-2,6 시알산전이효소를제공한다. 또한본 발명의시알산전이효소변이체는 3’- 및 6’-시알릴올리고당생산반응에서부반응감소, 생산성증대및 이로인한생산비용절감을제공한다.

公开(公告)号：KR1020150081185A

公开(公告)日：2015-07-13

申请号：KR1020140000906

申请日：2014-01-03

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  배진형 ,  이평강 ,  정은옥

IPC: C12N1/21 ,  C12N15/70

Abstract: 본발명은대장균 ()를이용한긴사슬지방산(long-chain fatty acid) 생변환을향상시키는것이다. 즉, 세포내에서긴사슬지방산의안정성을올리고세포외의기질은세포내로더 신속하게흡수되도록만듦으로써생변환수율을증가시키고생산성또한향상시킨다.

Abstract translation: 本发明的目的是通过使用大肠杆菌细菌改善长链脂肪酸生物转化。 也就是说，通过改善长链脂肪酸的稳定性和通过使细胞外基质更快地吸收到细胞中，生物转化产率提高并且提高生产力。

公开(公告)号：KR1020120005707A

公开(公告)日：2012-01-17

申请号：KR1020100066307

申请日：2010-07-09

Applicant: (주)아모레퍼시픽 ,  서울대학교산학협력단

Inventor： 박준성 ,  김덕희 ,  김한곤 ,  김은미 ,  김병기

IPC: C12P17/06 ,  C12P19/32 ,  C12N9/04

CPC classification number: C12P17/06 ,  C12N9/0006 ,  C12P19/32

Abstract: PURPOSE: A novel soil microorganism and oxidoreductase isolated from the microorganism are provided to ensure saccharide decomposition of natural products and to obtain various flavonoid aglycons. CONSTITUTION: A method for preparing flavonoid aglycons comprises a step of using Rhizobium sp. GIN611(KCTC11708BP) or cell extract thereof. The strain is isolated from soil. The flavonoid aglycon is prepared using Rhizobium sp. GIN611-derived oxidoreductase as a biocatalyst. The flavonoid aglycons are prepared by decomposing saccharides from flavonoid glycoside.

Abstract translation: 目的：提供从微生物分离的新型土壤微生物和氧化还原酶，以确保天然产物的糖分解并获得各种类黄酮苷。 构成：制备类黄酮苷元的方法包括使用根瘤菌属的步骤。 GIN611（KCTC11708BP）或其细胞提取物。 菌株与土壤分离。 使用根瘤菌（Rhizobium sp。）制备类黄酮苷元。 GIN611衍生的氧化还原酶作为生物催化剂。 通过从类黄酮苷分解糖类来制备黄酮苷。

公开(公告)号：KR1020100051758A

公开(公告)日：2010-05-18

申请号：KR1020080110718

申请日：2008-11-08

Applicant: 서울대학교산학협력단

Inventor： 이윤식 ,  김병기 ,  신동식 ,  김윤곤 ,  김은미 ,  김미라 ,  김재희

IPC: C12Q1/48 ,  G01N33/573

CPC classification number: C12Q1/485 ,  G01N2333/9121

Abstract: PURPOSE: A method for identifying protein kinase substrate specificity using a peptide library is provided to rapidly determining the substrate specificity of a protein phosphatase, and to examine the role of the protein phosphatase. CONSTITUTION: A method for identifying protein kinase substrate specificity comprises the following steps: forming a peptide library with a constant amino acid sequence on the surface of a solid support resin; forming a hybrid with randomized peptide libraries by repeating the previous step; reacting the peptide libraries with a phosphatase for forming the peptide library with phosphorylated threonine or serine; reacting an antibody combined with a biotin with the phosphorylated peptide library; reacting the obtained product with neutravidin or streptavidin including phosphatase or peroxidase; detecting the change of properties by the reaction of the phosphatase or the peroxidase for selecting the solid support resin with transformed peptide libraries; and an analyzing the amino acid sequence of the peptide after separating the peptide from the resin.

Abstract translation: 目的：提供使用肽文库鉴定蛋白激酶底物特异性的方法，以快速测定蛋白磷酸酶的底物特异性，并检查蛋白磷酸酶的作用。 构成：鉴定蛋白激酶底物特异性的方法包括以下步骤：在固体支持树脂的表面上形成具有恒定氨基酸序列的肽文库; 通过重复前述步骤与随机肽文库形成杂交体; 使肽文库与磷酸酶反应，用磷酸化苏氨酸或丝氨酸形成肽文库; 将与生物素结合的抗体与磷酸化肽文库反应; 使所得产物与中性链亲和素或链霉亲和素（包括磷酸酶或过氧化物酶）反应; 通过磷酸酶或过氧化物酶的反应检测特性的变化，用于用转化的肽文库选择固体支持树脂; 并分析肽与树脂分离后的氨基酸序列。