公开(公告)号：KR1019960010682A

公开(公告)日：1996-04-20

申请号：KR1019940022499

申请日：1994-09-07

Applicant: 동국제약 주식회사 ,  한국과학기술연구원

Inventor： 정봉현 ,  최의성 ,  손정훈 ,  윤덕중 ,  김명국 ,  이해돈 ,  이상기

IPC: C07K1/22 ,  C07K1/14

Abstract: 본 발명은 히루딘을 히루딘을 정제하는 방법을 제공한다. 본 발명의 방법은 조(粗) 히루딘액을 금속 이온으로서 구리이온을 사용하고 용리액으로서 인산염 완충액을 사용하는 금속이온 친화성 크로마토그래피를 이용하여 정제함을 특징으로 한다. 본 발명의 방법에 의하면 종래의 히루딘의 정제방법에 비해 저렴하고 간단하게 히루딘을 고순도로 정제할 수 있는 장점이 있다.

公开(公告)号：KR100068680B1

公开(公告)日：1993-12-13

申请号：KR1019910001231

申请日：1991-01-25

Applicant: 한국과학기술연구원

Inventor： 박영훈 ,  정봉현 ,  정수민 ,  손정덕 ,  신현호

IPC: C07D311/72

公开(公告)号：KR1019930005415B1

公开(公告)日：1993-06-21

申请号：KR1019910001231

申请日：1991-01-25

Applicant: 한국과학기술연구원

Inventor： 박영훈 ,  정봉현 ,  정수민 ,  손정덕 ,  신현호

IPC: C07D311/72

Abstract: The tocopherol is purifyed by (a) adding mixed solvent of n- hexane and 50 % (v/v) methanol into the soybean oil distillate, (b) removing the sterol of the mixt. by filtering, cooling the filtered soln., and re-filtering, (c) methyl esterifying the mixt. from which sterol is removed, (d) recirculating esterified product at 100-130 deg.C and 10-3-10-4 torr in the molecular distillator to remove fatty acid methyl ester, (e) concentrating the obtd. soln. contg. 50-55 % tocopherol and dissolving in the lower alcohol (I), (f) adsorbing it into alkaline anion exchange resin, and purifying with the (I) contg. 5-10 % acetic acid, and (g) dissolving the purifyed tocopherol in the (I) and passing through cation exchange resin to obtain the final product.

Abstract translation: 生育酚通过（a）将正己烷和50％（v / v）甲醇的混合溶剂加入大豆油馏出物中，（b）除去混合物的固醇来纯化。 通过过滤，冷却过滤的溶胶，并重新过滤，（c）甲基酯化混合物。 （d）在分子蒸馏器中在100-130℃和10-3-10-4乇下再循环酯化产物以除去脂肪酸甲酯，（e）浓缩该物质。 SOLN。 contg。 50-55％生育酚溶于低级醇（I）中，（f）将其吸附到碱性阴离子交换树脂中，用（I） 5-10％乙酸，和（g）将纯化的生育酚溶解在（I）中并通过阳离子交换树脂以获得最终产物。

公开(公告)号：KR100050067B1

公开(公告)日：1992-03-19

申请号：KR1019890000264

申请日：1989-01-12

Applicant: 한국과학기술연구원

Inventor： 박영훈 ,  정봉현 ,  정수민 ,  손정덕

IPC: C12P33/00

公开(公告)号：KR1019910007857B1

公开(公告)日：1991-10-02

申请号：KR1019890000264

申请日：1989-01-12

Applicant: 한국과학기술연구원

Inventor： 박영훈 ,  정봉현 ,  정수민 ,  손정덕

IPC: C12P33/00

Abstract: A process for preparing prednisolone from hydrocortisone comprises: (a) pre-culturing arthrobactor simplex at 30 deg.C, pH 7.3 and 150-180rpm in the medium to obtain the starter; (b) innoculating the starter to the culture medium contg. 0-0.2gr. glucose, 0.1-1.0gr. casamino acid, 0.05-0.5gr. KH2PO4, 0.05-0.5gr. K2HPO4, 0.001-0.01gr. Mgcl2, 0.05gr. tween 80 and 100ml D.W., and culturing at 30-37 deg.C, pH 6-9 and 150-180rpm for 4hrs.; (c) adding hydrocortisone powder into the culture broth and culturing at the same condition for 28hrs to obtain the final product. The prednisolone is useful as an antiphlogistic.

Abstract translation: 从氢化可的松制备泼尼松龙的方法包括：（a）在30℃，pH 7.3和150-180rpm下在培养基中预培养单克隆培养基以获得起子; （b）将起子接种到培养基中。 0-0.2gr。 葡萄糖，0.1-1.0gr。 酪蛋白氨基酸，0.05-0.5gr。 KH2PO4，0.05-0.5gr。 K2HPO4，0.001-0.01gr。 Mgcl2，0.05gr。 吐温80和100ml D.W.，并在30-37℃，pH 6-9和150-180rpm下培养4小时。 （c）将氢化可的松粉末加入到培养液中并在相同条件下培养28小时以获得最终产物。 泼尼松龙是有用的消炎药。

公开(公告)号：KR100031037B1

公开(公告)日：1990-01-08

申请号：KR1019870011375

申请日：1987-10-14

Applicant: 한국과학기술연구원

Inventor： 황운하 ,  장호남 ,  정봉현

IPC: C12M1/24

公开(公告)号：KR1020160135105A

公开(公告)日：2016-11-24

申请号：KR1020160059525

申请日：2016-05-16

Applicant: 재단법인 바이오나노헬스가드연구단 ,  한국과학기술연구원

Inventor： 김세훈 ,  정봉현 ,  김영선 ,  박경미 ,  서영훈

IPC: G01N33/58 ,  G01N33/543 ,  G01N21/64 ,  C07D487/04 ,  C07D409/14

Abstract: 본발명은 i) 선형업컨버전형광특성을가지는형광체를준비하고; ii) 상기형광체와생체물질을반응시켜서형광체와생체물질의반응복합체를형성하고; iii) 상기반응복합체를, 상기형광체의최대발광파장보다장파장의광원으로여기시키고; iv) 상기여기된반응복합체로부터방출되는, 상기여기광의파장보다단파장인발광신호를탐지하여측정하는; 단계를포함하는, 선형업컨버전형광특성을가진염료를이용한생체물질검출방법에관한것이다. 본발명은또한선형업컨버전형광특성을가진염료를이용한생체물질검출시스템및 키트에관한것이다.

公开(公告)号：KR1020000066750A

公开(公告)日：2000-11-15

申请号：KR1019990014065

申请日：1999-04-20

Applicant: 동국제약 주식회사 ,  한국과학기술연구원

Inventor： 정봉현 ,  오귀환 ,  함문선 ,  박진규

IPC: C07K19/00

CPC classification number: C07K14/395 ,  C07K2319/00 ,  C12N15/62 ,  C12N15/70 ,  C12Y304/16005

Abstract: PURPOSE: A novel confusion partner, the carboxypeptidase Y(CPY) propeptide for effective representation of polypeptide in E.coli, a recombinant vector and its transformed body are provided, by which small polypeptides(that is degradable easily in E.coli) can be produced stably. CONSTITUTION: A process for the preparation of novel confusion partner, the carboxypeptidase Y(CPY) propeptide, a recombinant vector and its transformed body comprises of: separating genomic DNA of Saccharomyces serebisiae, and processing PCR(Polymerase Chain Reaction) using the separated genomic DNA as a model chain(the primer used in this stage is synthesized by a DNA synthesizer); separating the cut fraction from agarose gel after cutting the fraction(fraction 1: 287 bp) gained by PCR with a restriction enzyme Nde I and Xho I, inserting into pET22b+ vector, and transforming to E.coli BL21(DE3) to represent; and inoculating the transformed E.coli and not-transformed E.coli to LB(yeast extract 0.5%, trypton 1%, NaCl 1%) liquid medium containing ampicillin, and confirming representation by adding IPTG(Isopropyl-beta-D-thiogalactopyranoside) while culturing at 37°C. The CPY propeptide is existed in the form of non-soluble coagulated body, but is dissolved in urea easily.

Abstract translation: 目的：提供一种新颖的混淆对象，用于在大肠杆菌中有效表达多肽的羧肽酶Y（CPY）前体肽，重组载体及其转化体，通过其可以将小多肽（易于在大肠杆菌中降解）可以是 稳定生产。 构成：一种制备新的混淆配偶体的方法，羧肽酶Y（CPY）前肽，重组载体及其转化体包括：分离酵母菌的基因组DNA，并使用分离的基因组DNA进行PCR（聚合酶链式反应） 作为模型链（本阶段使用的引物由DNA合成仪合成）; 在用限制性内切酶Nde I和Xho I进行PCR后，切割通过PCR获得的级分（级分1：287bp），将切割的级分与琼脂糖凝胶分离，插入pET22b +载体中，并转化至大肠杆菌BL21（DE3） 并将转化的大肠杆菌和未转化的大肠杆菌接种到含有氨苄青霉素的LB（酵母提取物0.5％，1％，1％盐酸1％）液体培养基中，并通过加入IPTG（异丙基-β-D-硫代吡喃半乳糖苷） 同时在37℃培养。 CPY前肽以不溶性凝固体的形式存在，但易溶于尿素。

公开(公告)号：KR1020000056647A

公开(公告)日：2000-09-15

申请号：KR1019990006137

申请日：1999-02-24

Applicant: 한국과학기술연구원

Inventor： 정봉현 ,  김민곤 ,  김철호 ,  최순자 ,  이상기

IPC: C12N9/20

CPC classification number: C12N9/20 ,  C07K1/14

Abstract: PURPOSE: Lipase purified with an organic solvent, its preparation method and chiral medical and pharmaceutical products using the lipase are provided, which lipase is improved in the enantiomeric ratio and is useful to prepare optically pure (S)-ketoprofen, (S)-ibuprofen and (S)-suprofen. CONSTITUTION: A lipase with improved enantiomeric ratio is prepared by adding 0.3-1.0 times by volume of an organic solvent to Candida rugosa lipase solution to collect the first-stage precipitate; adding 0.3-1.0 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the second-stage precipitate; and 0.8-1.2 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the precipitate. The organic solvent is acetone or isopropane.

Abstract translation: 目的：提供用有机溶剂纯化的脂肪酶，其制备方法和使用脂肪酶的手性医药产品，该脂肪酶的对映体比例得到改善，可用于制备光学纯的（S） - 酮洛芬，（S） - 布洛芬 和（S） - 布洛芬。 构成：通过向假丝酵母脂肪酶溶液中加入0.3-1.0倍体积的有机溶剂来收集第一阶段沉淀物来制备具有改善的对映异构体比例的脂肪酶; 将基于原始脂肪酶溶液的体积的有机溶剂的0.3-1.0倍体积添加到上清液中，以收集第二阶段沉淀物; 和0.8-1.2倍体积的有机溶剂，基于原始脂肪酶溶液到上清液的体积，收集沉淀物。 有机溶剂为丙酮或异丙醇。

公开(公告)号：KR100262769B1

公开(公告)日：2000-08-01

申请号：KR1019970074490

申请日：1997-12-26

Applicant: 한국과학기술연구원

Inventor： 이상기 ,  정봉현 ,  김원경 ,  송기방 ,  김철호

IPC: C07K1/14

Abstract: PURPOSE: Provided is an aqueous and hetero phase separation segregate system consisting of a high molecular aqueous solution of polyethylene glycol and levan, to separate proteins in large quantity with low cost. And a protein separation system using the same is also provided. CONSTITUTION: An aqueous and hetero phase separation segregate system consisting of a high molecular aqueous solution of polyethylene glycol and levan is composed of the following steps of: (a) preparing polyethylene glycol-levan hetero phase segregation system; (b) searching phase diagram of polyethylene glycol-levan; and (c) searching distribution coefficient according to the change of concentration of salt and pH value.

Abstract translation: 目的：提供由高分子量聚乙二醇水溶液和levan组成的水相和异相分离分离系统，以低成本大量分离蛋白质。 还提供了使用其的蛋白质分离系统。 构成：由高分子量聚乙二醇水溶液和levan组成的水相和异相分离分离系统由以下步骤组成：（a）制备聚乙二醇 - 异戊二烯异相分离系统; （b）搜索聚乙二醇 - 叶子的相图; 和（c）根据盐浓度和pH值的变化搜索分布系数。