아연을 이용한 단백질 구조 결정 방법
    42.
    发明公开
    아연을 이용한 단백질 구조 결정 방법 有权
    使用ZINC测定蛋白质结构的方法

    公开(公告)号:KR1020140095288A

    公开(公告)日:2014-08-01

    申请号:KR1020130008091

    申请日:2013-01-24

    Abstract: The present invention relates to a method of solving a phase by using zinc. More specifically, the present invention comprises: a step of contacting foreign zinc to a protein not having a zinc binding point; a step of irradiating an x-ray to the protein and foreign zinc; and a step of observing an anomalous signal of foreign zinc. The present invention originally does not have zinc, thus enables an x-ray analysis of a heavy element by contacting foreign zinc to the protein in which x-ray analysis of heavy element was impossible. Therefore, the present invention expands drastically a range of protein enabling the x-ray analysis of the heavy element, allows to analyze nearly all protein, easily reveals structures of the protein, and can facilitate the analysis of the heavy element of a pre-crystallized protein.

    Abstract translation: 本发明涉及通过使用锌来求解相的方法。 更具体地说,本发明包括:使外来锌与不具有锌结合点的蛋白质接触的步骤; 将X射线照射到蛋白质和外来锌的步骤; 并观察外来锌异常信号的一个步骤。 本发明最初不具有锌,因此能够通过使外来的锌与不可能重元素的X射线分析的蛋白质接触而对重元素进行x射线分析。 因此,本发明大幅度地扩展了能够进行重元素的X射线分析的蛋白质的范围,允许分析几乎所有的蛋白质,容易地揭示蛋白质的结构,并且可以促进对预结晶的重元素的分析 蛋白。

    혐기성 미생물을 이용한 수소 생산 장치
    46.
    发明公开
    혐기성 미생물을 이용한 수소 생산 장치 有权
    使用无机微生物生产氢的装置

    公开(公告)号:KR1020130115495A

    公开(公告)日:2013-10-22

    申请号:KR1020120037822

    申请日:2012-04-12

    Abstract: PURPOSE: A hydrogen producing device using anaerobic microorganisms is provided to enhance hydrogen production efficiency to allow hydrogen-producing anaerobic microorganisms to use carbon monoxides when hydrogen is produced. CONSTITUTION: A hydrogen producing device (100) using anaerobic microorganisms comprises: a culture machine (110) which stores a culture medium containing hydrogen-producing anaerobic microorganisms; a circulation line (120) which is connected to the culture machine to discharge the culture medium from the culture machine and has a pump (121) for circulation of the culture medium; a carbon monoxide supply unit (130) which supplies carbon monoxides to the culture medium; a dissolution unit (140) with a tube which provides a delayed path for delaying time for dissolving the carbon monoxides; and a hydrogen collection unit (150) which captures hydrogen from gas separated from the culture medium. [Reference numerals] (130) Carbon monoxide supplying unit; (148) Control unit; (150) Hydrogen collecting unit; (160) Cooling unit; (170) Culture medium supplying unit

    Abstract translation: 目的:提供使用厌氧微生物的氢气生产装置,以提高制氢效率,使氢气产生厌氧微生物在生产氢气时使用一氧化碳。 构成:使用厌氧微生物的制氢装置(100)包括:储存含有产氢厌氧微生物的培养基的培养机(110) 与培养机连接以从培养机排出培养基的循环管线(120),并具有用于培养培养基循环的泵(121); 向培养基供给一氧化碳的一氧化碳供给单元(130) 溶解单元(140),其具有提供用于延迟溶解所述一氧化碳的时间的延迟路径的管; 和从所述培养基分离的气体捕获氢的氢气收集单元(150)。 (附图标记)(130)一氧化碳供给单元; (148)控制单元; (150)集氢单元; (160)冷却单元; (170)培养基供给单元

    론 단백질을 포함하는 고온 생장 가능한 재조합 미생물 및 열-쇼크 단백질을 이용한 바이러스의 열안정화 방법
    47.
    发明公开
    론 단백질을 포함하는 고온 생장 가능한 재조합 미생물 및 열-쇼크 단백질을 이용한 바이러스의 열안정화 방법 有权
    用于高温生长的重组微生物包括蛋白质和使用热休克蛋白进行病毒稳定的方法

    公开(公告)号:KR1020130114025A

    公开(公告)日:2013-10-16

    申请号:KR1020130100953

    申请日:2013-08-26

    Abstract: PURPOSE: A recombinant microorganism is provided to show activation and growth at high temperatures and to enable culturing at high temperatures, thereby being widely used. CONSTITUTION: A recombinant microorganism which grows at high temperatures contains lon proteins of sequence number 4. The microorganism is prokaryotes. The microorganism is gram-negative bacteria, E. coli. Virus is thermally stabilized using lon protein of sequence number 4. A thermal stabilization method of the virus comprises the steps of: isolating lon protein of sequence number 4; and making the isolated proteins and the virus come in contact. [Reference numerals] (AA) Measure survivability; (BB) Measure growth

    Abstract translation: 目的:提供重组微生物以显示高温下的活化和生长,并能够在高温下培养,从而被广泛使用。 构成:在高温下生长的重组微生物含有4号序列号4的蛋白质。微生物是原核生物。 微生物是革兰氏阴性细菌,大肠杆菌。 病毒使用序列号4的蛋白质热稳定。病毒的热稳定方法包括以下步骤:分离序列号4的蛋白质; 并使分离的蛋白质和病毒接触。 (附图标记)(AA)测量生存性; (BB)测量增长

    해양 미생물에서 분리한 신규한 DNA 메틸라아제 및 이의 제조방법
    48.
    发明公开
    해양 미생물에서 분리한 신규한 DNA 메틸라아제 및 이의 제조방법 审中-实审
    从海洋微生物中分离的新型DNA甲基化酶及其生产方法

    公开(公告)号:KR1020170084634A

    公开(公告)日:2017-07-20

    申请号:KR1020160003903

    申请日:2016-01-12

    Abstract: 본발명은해양미생물알파프로박테리아균주 IMCC12053로부터분리된신규의외향고리 DNA 아미노그룹메틸라아제및 이의제조방법에관한것으로서, 해양미생물에서동정된 DNA 메틸라아제및 이를암호화하는 DNA 메틸라아제유전자를제공한다. 유전자를발현재조합벡터에클로닝하고형질전환된세포에서발현하여 DNA 메틸라아제를대량생산및 분리하는방법을제공하고있다. 본발명의신규 DNA 메틸트랜스퍼라아제는제한효소자리보호하고 DNA의물리적물성변화를유도하여제한효소인식서열을감소시킴으로써효소특이성을증가시키는효과를제공한다.

    Abstract translation: 本发明是一个,一个DNA甲基LA激酶和DNA甲基拉脱水酶基因从海洋细菌编码相同孤立涉及一种海洋微生物的α-临细菌菌株甲基丙环向外从IMCC12053 LA激酶和它们的制备中分离的新颖的DNA的氨基 提供。 将基因克隆到表达重组载体中并在转化的细胞中表达以大量生产并分离DNA甲基化酶。 根据本发明的La氮杂新的DNA甲基转移提供了一种限制性内切酶保护位置的效果,通过减少的限制酶识别序列以诱导DNA的物理性质的物理变化更大的酶的特异性。

    신규한 프로모터 및 이를 이용한 수소 생산 방법
    50.
    发明授权
    신규한 프로모터 및 이를 이용한 수소 생산 방법 有权
    新型促进剂及其使用方法

    公开(公告)号:KR101684489B1

    公开(公告)日:2016-12-09

    申请号:KR1020150071782

    申请日:2015-05-22

    Abstract: 본발명은신규한프로모터및 이를이용한수소생산방법에관한것으로서, RNA sequencing을사용한전사체해석(transcriptome analysis)을통하여우리는새로운내생성의강한프로모터를제공한다. 본발명은또한상기프로모터를이용한단백질생산방법을제공한다. 본발명은또한상기프로모터를포함하는재조합벡터및 상기재조합벡터로치환된숙주세포를제공한다. 본발명은상기숙주세포를이용한수소생산방법을제공한다. 본발명에따른프로모터는종래에알려진어떠한프로모터보다더 강력하다. 따라서본 발명에따른프로모터로치환된 균주는수소생산능력에서종래의야생형대비현저하게높은수소생산율을가진다. 따라서본 발명에따라제조된돌연변이NA1는높은수소생산율뿐만아니라높은균주생장율을가지고있어수소생산효율이크게증가된균주이다.

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