Abstract:
본 발명은 신규의 에스터라제 및 이를 암호화하는 유전자, 및 이를 이용한 에스터라제 생산방법에 관한 것으로, 본 발명에 따른 에스터라제는 고온에서도 높은 활성을 나타내고, 높은 pH에서도 에스터라제 활성이 있다. 따라서 본 발명에 따른 에스터라제는 세제, 식품 및 의약품 생산에 유용하게 활용될 수 있다.
Abstract:
The present invention relates to a method of solving a phase by using zinc. More specifically, the present invention comprises: a step of contacting foreign zinc to a protein not having a zinc binding point; a step of irradiating an x-ray to the protein and foreign zinc; and a step of observing an anomalous signal of foreign zinc. The present invention originally does not have zinc, thus enables an x-ray analysis of a heavy element by contacting foreign zinc to the protein in which x-ray analysis of heavy element was impossible. Therefore, the present invention expands drastically a range of protein enabling the x-ray analysis of the heavy element, allows to analyze nearly all protein, easily reveals structures of the protein, and can facilitate the analysis of the heavy element of a pre-crystallized protein.
Abstract:
본 발명은 수소생산능이 증가된 써모코코스 온누리우스 엔에이원 돌연변이 균주 및 이를 이용한 수소생산방법에 관한 것으로, 보다 구체적으로는 CO 디하이드로게나아제, 하이드로게나아제( mch ) 및 복합-구조체 Na + /H + 안티포터(antiporter) ( mnh3 )유전자 클러스터의 발현율이 증가된 것을 특징으로 하는 수소생산능력이 증가된 Thermococcus
Abstract:
본 발명은 심해 해저에서 분리된 리파제 및 에스터라제 효소 및 이의 제조방법에 관한 것으로서, 서남태평양 해역(남위 3°89′, 동경 152°49′) 깊이 1,400 미터에 이르는 심해 퇴적물, 북극 다산기지 주변 연안 퇴적물, 강화도 갯벌 퇴적물로부터 구축된 메타게놈 라이브러리를 대상으로 분리한 신규 리파제 및 에스터라제 효소, 이들을 암호화 하는 유전자 및 리파제 및 에스터라제 효소 생산방법을 제공한다. 리파제, 에스터라제
Abstract:
The present invention relates to a mutated thermococcus onnurineus NA1 with increased hydrogen productivity and a method for producing hydrogen using the same, and more specifically, to a mutated thermococcus onnurineus NA1 with increased hydrogen productivity and a method for producing hydrogen using the same, wherein the expression of a CO dehydrogenase, a hydrogenase (mch), a complex-structure Na+/H+ antiporter(mnh3) gene cluster is improved.
Abstract translation:本发明涉及具有增加的氢生产率的突变型热带炎热球菌NA1和使用其的氢的制造方法,更具体地说,涉及具有增加的氢生产率的突变的on鱼属NA1和使用其的氢的制造方法,其中 CO脱氢酶,氢化酶(mch),复合结构Na + / H +反转录酶(mnh3)基因簇的表达得到改善。
Abstract:
PURPOSE: A hydrogen producing device using anaerobic microorganisms is provided to enhance hydrogen production efficiency to allow hydrogen-producing anaerobic microorganisms to use carbon monoxides when hydrogen is produced. CONSTITUTION: A hydrogen producing device (100) using anaerobic microorganisms comprises: a culture machine (110) which stores a culture medium containing hydrogen-producing anaerobic microorganisms; a circulation line (120) which is connected to the culture machine to discharge the culture medium from the culture machine and has a pump (121) for circulation of the culture medium; a carbon monoxide supply unit (130) which supplies carbon monoxides to the culture medium; a dissolution unit (140) with a tube which provides a delayed path for delaying time for dissolving the carbon monoxides; and a hydrogen collection unit (150) which captures hydrogen from gas separated from the culture medium. [Reference numerals] (130) Carbon monoxide supplying unit; (148) Control unit; (150) Hydrogen collecting unit; (160) Cooling unit; (170) Culture medium supplying unit
Abstract:
PURPOSE: A recombinant microorganism is provided to show activation and growth at high temperatures and to enable culturing at high temperatures, thereby being widely used. CONSTITUTION: A recombinant microorganism which grows at high temperatures contains lon proteins of sequence number 4. The microorganism is prokaryotes. The microorganism is gram-negative bacteria, E. coli. Virus is thermally stabilized using lon protein of sequence number 4. A thermal stabilization method of the virus comprises the steps of: isolating lon protein of sequence number 4; and making the isolated proteins and the virus come in contact. [Reference numerals] (AA) Measure survivability; (BB) Measure growth
Abstract:
본발명은해양미생물알파프로박테리아균주 IMCC12053로부터분리된신규의외향고리 DNA 아미노그룹메틸라아제및 이의제조방법에관한것으로서, 해양미생물에서동정된 DNA 메틸라아제및 이를암호화하는 DNA 메틸라아제유전자를제공한다. 유전자를발현재조합벡터에클로닝하고형질전환된세포에서발현하여 DNA 메틸라아제를대량생산및 분리하는방법을제공하고있다. 본발명의신규 DNA 메틸트랜스퍼라아제는제한효소자리보호하고 DNA의물리적물성변화를유도하여제한효소인식서열을감소시킴으로써효소특이성을증가시키는효과를제공한다.