公开(公告)号：AT371028T

公开(公告)日：2007-09-15

申请号：AT00951461

申请日：2000-07-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12M1/40 ,  C12N15/53 ,  C12N1/21 ,  C12N9/02 ,  C12N15/09 ,  C12N15/70 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/42 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/11 ,  C12R1/19

Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.

公开(公告)号：DE502004002309D1

公开(公告)日：2007-01-25

申请号：DE502004002309

申请日：2004-05-05

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  HABICHER TILO ,  SCHMID ROLF ,  MAURER CHRISTIAN ,  URLACHER BENIAMINOVNA ,  SCHULZE HOLGER ,  HUBER NORBERT ,  BACHMANN T

IPC: C12N9/02 ,  C12Q1/26

Abstract: A process for preparing a hydroxylation catalyst by i) embedding a cystochrome P450 monooxygenase in a sol-gel matrix, ii) embedding an enzymatic NADPH-regenerating system in a sol-gel matrix, and combining the two components i) and ii) unless they were already mixed together before the embedding.

公开(公告)号：MY126592A

公开(公告)日：2006-10-31

申请号：MYPI20003409

申请日：2000-07-26

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12N15/09 ,  C12N15/53 ,  C12M1/40 ,  C12N1/21 ,  C12N9/02 ,  C12N15/70 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/64 ,  C12P17/06 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/19

Abstract: THE INVENTION RELATE NOVEL CYTOCHROME P450 MONOXYGENASES WITH ALTERED SUBSTRATE SPECIFY WHICH ARE CAPABLE OF THE OXIDATION OF ORGANIC COMPOUNDS AS , FOR EXAMPLE, N-HETEROCYCLIC AROMATIC COMPOUNDS, NUCLEOTIDE SEQUENCE CODING THEREFOR, EXPRESSION CONSTRUCTS AND VECTORS COMPRISING THESE SEQUENCES, MICROORGANISMS TRANSFORMED THEREWITH, PROCESSES FOR THE MICROBIOLOGICAL OXIDATION OF SAID ORGANIC COMPOUNDS AND IN PARTICULAR PROCESSES FOR THE PREPARATION OF INDIGO AND INDIRUBIN.

公开(公告)号：AU784049B2

公开(公告)日：2006-01-19

申请号：AU6439000

申请日：2000-07-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12M1/40 ,  C12N9/00 ,  C12N1/21 ,  C12N9/02 ,  C12N15/09 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/42 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/11 ,  C12R1/19

Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.

公开(公告)号：AU2003253323A1

公开(公告)日：2004-02-23

申请号：AU2003253323

申请日：2003-07-25

Applicant: BASF AG

Inventor： MATUSCHEK MARKUS ,  HAUER BERNHARD ,  SCHMID ROLF ,  KAUFFMANN ISABELLE MELANIE ,  BLASCO FRANCESCA ,  SCHMIDT-DANNERT CLAUDIA

IPC: C12N9/02 ,  C12P23/00

Abstract: The invention relates to a process for the biotransformation of carotenoids using enzymes having cytochrome P450 monooxygenase activity; in particular monooxygenases from thermophilic bacteria, in particular of the genus Thermus sp.

公开(公告)号：BR0012772A

公开(公告)日：2002-04-02

申请号：BR0012772

申请日：2000-07-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN ,  LUTZ-WAHL SABINE ,  APPEL DANIEL

IPC: C12N15/09 ,  C12M1/40 ,  C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/19 ,  C12N15/70

Abstract: The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.

公开(公告)号：NO20020350L

公开(公告)日：2002-01-23

申请号：NO20020350

申请日：2002-01-23

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12N1/21 ,  C12M1/40 ,  C12N9/02 ,  C12N15/09 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/42 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/11 ,  C12R1/19 ,  C12N

Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.

公开(公告)号：NO20020350D0

公开(公告)日：2002-01-23

申请号：NO20020350

申请日：2002-01-23

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEISS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12N1/21 ,  C12M1/40 ,  C12N9/02 ,  C12N15/09 ,  C12P7/02 ,  C12P7/04 ,  C12P7/22 ,  C12P7/42 ,  C12P7/64 ,  C12P17/10 ,  C12P17/16 ,  C12Q1/26 ,  C12R1/11 ,  C12R1/19 ,  C12N

Abstract: The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.

公开(公告)号：DE10011723A1

公开(公告)日：2001-09-13

申请号：DE10011723

申请日：2000-03-10

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  PLEIS JUERGEN ,  SCHWANEBERG ULRICH ,  SCHMITT JUTTA ,  FISCHER MARKUS ,  SCHMID ROLF ,  LI QING-SHAN

IPC: C12N9/02 ,  C12N15/53 ,  C12P7/42 ,  C12P7/64 ,  C12Q1/26 ,  C12N15/70

Abstract: An electron donor system (M1) that transfers electrons to an enzyme with redox properties comprising an inorganic, not electrode-bound, electron sink and a mediator which enable the electron transfer is new. Independent claims are also included for: (1) transferring (M2) oxygen to a hydrocarbon containing hydrogen donor molecule, where the hydrogen donor molecule is in a reaction medium comprising the oxygen transferring enzyme and (M1) in the presence of oxygen and incubating under suitable reaction conditions; (2) the enzymatic production (M3) of terminally or subterminally hydroxylated (position w-1 to w-4) fatty acids comprising: (i) mixing a hydroxylatable fatty acid or fatty acid derivative in the presence of (M1) and cytochrome P450 monooxygenase; and (ii) isolating the hydroxylated product; (3) a bioreactor useful for the production of w-hydroxylated fatty acids as described in (2); and (4) detecting (M4) fatty acid-monooxygenases comprising: (i) contacting the analyte with a w-hydroxylatable fatty acid or a derivative comprising a terminal chromophore or fluorescent label in (M1); and (ii) qualitatively or quantitatively detecting the signal.